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Rapid propagation method of purple stem tissue culture

A tissue culture, purple stem technology, applied in the field of plant reproduction, to avoid damage, promote proliferation, and reduce the probability of producing deformed seedlings

Active Publication Date: 2018-08-07
陈思
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the tissue culture research of Adenophora

Method used

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  • Rapid propagation method of purple stem tissue culture

Examples

Experimental program
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Effect test

Embodiment 1

[0051] A method for rapid propagation of purple stem tissue culture, comprising the following steps:

[0052] A, select the purple stem plant with excellent character as the female parent, cut its new shoots in the same year, get the branches of its top 5cm, soak the branches with a mass fraction of 2% detergent solution for 20min, take them out, and use a thread Rinse with tap water for 15min, then put into natural disinfectant and soak for 30min, finally rinse the branch with sterile water for 3 times and remove its surface moisture with sterile filter paper to obtain explant, wherein,

[0053] The preparation method of the natural disinfectant is as follows: crush 10 parts by weight of Daidai, 7 parts by weight of Melaleuca mellifera, 7 parts by weight of Eucalyptus blueus, 6 parts by weight of garlic, 5 parts by weight of lemongrass and 4 parts by weight of cloves to 50 mesh, Add 500 parts by weight of water to soak at room temperature for 1 hour, then vacuum reflux extrac...

Embodiment 2

[0075] A method for rapid propagation of purple stem tissue culture, comprising the following steps:

[0076] A, select the purple stem plant with excellent character as the female parent, cut its new shoots in the same year, get the branches of its top 8cm, soak the branches with a mass fraction of 2% detergent solution for 20min, take them out, and use a thread Rinse with tap water for 18min, then put into natural disinfectant and soak for 30min, finally rinse the branch with sterile water 4 times and remove its surface moisture with sterilized filter paper to obtain explant, wherein,

[0077]The preparation method of the natural disinfectant is as follows: crush 11 parts by weight of Daidai, 8 parts by weight of Melaleuca mellifera, 8 parts by weight of Eucalyptus eucalyptus, 7 parts by weight of garlic, 6 parts by weight of lemongrass and 5 parts by weight of cloves to 80 mesh, Add 500 parts by weight of water to soak at room temperature for 1 hour, then vacuum reflux at 6...

Embodiment 3

[0099] A method for rapid propagation of purple stem tissue culture, comprising the following steps:

[0100] A, select the purple stem plant with excellent character as the female parent, cut its new shoots in the same year, get the branches at the top 10cm, soak the branches with a mass fraction of 2% detergent solution for 20min, take them out, and use a thread Rinse with tap water for 20min, then put into natural disinfectant and soak for 30min, finally rinse the branch with sterile water for 5 times and remove its surface moisture with sterile filter paper to obtain the explant, wherein,

[0101] The preparation method of the natural disinfectant is as follows: crush 12 parts by weight of Daidai, 9 parts by weight of Melaleuca mellifera, 9 parts by weight of Eucalyptus eucalyptus, 8 parts by weight of garlic, 7 parts by weight of lemongrass and 6 parts by weight of cloves to 100 mesh, Add 500 parts by weight of water to soak at room temperature for 1 hour, then vacuum ref...

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Abstract

The invention provides a tissue-culture rapid-breeding method for stewartia sinensis. The tissue-culture rapid-breeding method for the stewartia sinensis comprises the steps of explant selection and disinfection, cluster bud induction, cluster bud proliferation, rooting culture, strong seedling culture and seedbed culture. Compared with the traditional seedling culture method of the stewartia sinensis, the tissue-culture rapid-breeding method for the stewartia sinensis has the following advantages: the method is not limited by the number and the germination rate of of stewartia sinensis seeds; annual young shoots of the stewartia sinens are subjected to tissue culture to obtain stewartia sinensis seedlings, and the stewartia sinensis seedlings are transplanted into seedbeds to be continuously cultured, thereby obtaining stewartia sinensis transplanted seedlings; the tissue-culture rapid-breeding method for the stewartia sinensis can effectively improve the breeding coefficient and retains the excellent hereditary character of the stewartia sinensis, the rate of survival of seedlings is as high as 95%, the rate of survival of field transplated seedlings is as high as 80%, and large-scale production and field large-area forestation growth of stewartia sinensis nursery stocks can be achieved; and the invention provides technical supports for effective protection of the stewartia sinensis which is the rare and endangered plant.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a method for rapid propagation of purple stem tissue culture. Background technique [0002] Stewartia sinensis (Latin name: Stewartia sinensis Rehd. et Wils.) belongs to the perennial woody plant of the genus Stewartia sinensis in the family Theaceae. Due to the continuous destruction of vegetation and poor natural regeneration, the purple stem plants have been decreasing day by day, and are listed as "endangered species" in the Chinese Red Book. Purple stem is a unique relic plant in China, which is of scientific significance to the study of East Asia-North America flora. Its bark is flaky and detached, the inner bark is brown and yellow, smooth, mottled and beautiful, and the flowers are white and yellow. Courtyard; the material is heavy, durable and beautiful in color, and it is an excellent material for making furniture; the root bark, stem bark, and fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02A40/22Y02P60/40
Inventor 陈思
Owner 陈思
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