A method for cultivating virus-free strawberry seedlings using tissue culture technology
A technology for detoxifying seedlings and strawberries, which is applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of low transplant survival rate, low reproduction coefficient, poor detoxification effect, etc. The effect of planting management, high transplant survival rate, and labor cost reduction
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Embodiment 1
[0029] A kind of method utilizing tissue culture technology to cultivate virus-free strawberry seedlings described in the present embodiment comprises the following steps:
[0030] (1) Selection and sterilization of explants: collect unopened flower buds of strawberry plants of Xuemei variety, the size of the flower buds is 0.4-0.6 mm, first sterilize with 75% alcohol for 20 seconds, and then use 0.1% mercuric chloride solution Sterilize for 13 minutes, take out the anthers, and obtain explants;
[0031] (2) Cluster bud induction: inoculate the explants obtained in step (1) into medium M1, culture in the dark for 9 days, and then cultivate in light for 80 days, until cluster shoots are produced, and the bud height of the cluster buds is not less than 2 cm. The culture conditions are culture light 2000-3000LX, culture temperature 24-26°C, and light duration 16h / day; the composition of the medium M1 is: MS basic medium supplemented with 1.0mg / L TDZ and 0.2mg / L IAA;
[0032] (3)...
Embodiment 2
[0035] A kind of method utilizing tissue culture technology to cultivate strawberry virus-free seedlings described in the present embodiment differs from the method described in Example 1 in that:
[0036] Step (2) clustering bud induction: Inoculate the explants obtained in step (1) into medium M1, culture in the dark for 9 days, then light culture for 80 days, until clustering buds are produced, and the height of the clustering buds is not less than 2cm, the culture conditions are culture light 2000-3000LX, culture temperature 24-26℃, light duration 16h / day; the composition of the medium M1 is: MS basic medium, supplemented with 1.5mg / L TDZ, 0.1mg / L IAA.
[0037]The method described in this example cultivates virus-free strawberry seedlings. When the explants are inoculated in medium M1 for culture, the callus generation time is 9 days of dark culture and then 10 days of light culture, and buds appear after 60 days of light culture. After 80 days of cultivation, the bud poi...
Embodiment 3
[0039] A kind of method utilizing tissue culture technology to cultivate strawberry virus-free seedlings described in the present embodiment differs from the method described in Example 1 in that:
[0040] Step (2) clustering bud induction: Inoculate the explants obtained in step (1) into medium M1, culture in the dark for 9 days, then light culture for 80 days, until clustering buds are produced, and the bud height of the clustering buds is not less than 2cm, the culture conditions are culture light 2000-3000LX, culture temperature 24-26℃, light duration 16h / day; the composition of the medium M1 is: MS basic medium, supplemented with 1.0mg / L TDZ, 0.2mg / L IAA.
[0041] The method described in this example cultivates virus-free strawberry seedlings. When the explants are inoculated in medium M1 for culture, the callus generation time is 9 days of dark culture and then 10 days of light culture, and buds appear after 60 days of light culture. After 80 days of cultivation, the bu...
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