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Immobilized multi-enzyme system and preparation method thereof

A multi-enzyme system and mixed-enzyme technology, which can be applied in the direction of immobilization on or in inorganic carriers, chemical instruments and methods, water pollutants, etc. To achieve the effect of short reaction time, good stability and large solid load

Inactive Publication Date: 2020-06-23
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides an immobilized multi-enzyme system and a preparation method thereof, which combine ultraviolet radiation with a metal-organic framework, are easy to operate, have mild curing reaction conditions, and have high curing efficiency , effectively solve the problems of low degradation rate of organic matter, small solid load and poor stability, suitable for large-scale production, and can enhance the treatment effect of sludge lysis conditioning or organic wastewater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of an immobilized multi-enzyme system includes the following steps:

[0032] (1) Add 0.0075mol / L nickel nitrate solution and 0.025mol / L ferric nitrate solution to 0.02mol / L terephthalic acid solution and 0.02mol / L dimethylformamide solution at a molar ratio of 1:1. Add hydrofluoric acid to adjust the pH value of the solution to 2.5±0.5, and then perform ultrasonic dispersion. After 30 minutes of ultrasonic dispersion and mixing at 200W power, the reaction is followed by hydrothermal reaction at 130°C. 72h, then cooled to room temperature and suction filtered at room temperature, rinsed with N,N-dimethylformamide solution for 3 times, vacuum dried at 60℃ for 12h to obtain intermediate products; among them, nickel nitrate solution, iron nitrate solution and The volume ratio of terephthalic acid solution is 0.75:1.25:1;

[0033] (2) Dissolve the intermediate product obtained in step (1) in N,N-dimethylformamide solution, purify it at 150°C for 36h, and the...

Embodiment 2

[0038] A preparation method of an immobilized multi-enzyme system includes the following steps:

[0039] (1) Add 0.0065mol / L nickel nitrate solution and 0.038mol / L ferric nitrate solution to 0.025mol / L terephthalic acid solution and 0.025mol / L dimethylformamide solution at a molar ratio of 1:1 Add hydrofluoric acid to adjust the pH value of the solution to 2.5±0.5 and then perform ultrasonic dispersion. After ultrasonic dispersion and mixing for 48 minutes under 200W power, the reaction is followed by hydrothermal reaction at 130°C. 80h, then cooled to room temperature, suction filtered at room temperature, rinsed with N,N-dimethylformamide solution 3 times, vacuum dried at 60℃ for 12h, to obtain intermediate product; among them, nickel nitrate solution, iron nitrate solution and The volume ratio of terephthalic acid solution is 0.8:1.3:1;

[0040] (2) Dissolve the intermediate product obtained in step (1) in N,N-dimethylformamide solution, purify it at 150°C for 36h, and then in ...

Embodiment 3

[0045] A preparation method of an immobilized multi-enzyme system includes the following steps:

[0046] (1) Add 0.007mol / L nickel nitrate solution and 0.036mol / L ferric nitrate solution to 0.02mol / L terephthalic acid solution and 0.02mol / L dimethylformamide solution at a molar ratio of 1:1. Add hydrofluoric acid to adjust the pH of the solution to 2.5±0.5, and then perform ultrasonic dispersion. After ultrasonic dispersion and mixing at 200W power for 48 minutes, the reaction is followed by hydrothermal reaction at 130°C. 80h, then cooled to room temperature, suction filtered at room temperature, rinsed with N,N-dimethylformamide solution 3 times, vacuum dried at 60℃ for 12h, to obtain intermediate product; among them, nickel nitrate solution, iron nitrate solution and The volume ratio of terephthalic acid solution is 0.8:1.28:1;

[0047] (2) Dissolve the intermediate product obtained in step (1) in N,N-dimethylformamide solution, purify it at 150°C for 36h, and then in turn suct...

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Abstract

The invention discloses an immobilized multi-enzyme system and a preparation method thereof. The preparation method comprises the following steps: adding a nickel nitrate solution and a ferric nitratesolution into a mixed solution, adjusting the pH value, carrying out hydrothermal reaction after ultrasonic dispersion, and performing cooling to room temperature to obtain an intermediate product; dissolving the intermediate product into an organic solvent, sequentially carrying out suction filtration, washing and vacuum drying to obtain a metal organic framework compound; preparing a mixed enzyme solution; and adding the metal organic framework compound into the mixed enzyme solution, and performing irradiating by ultraviolet rays to obtain the immobilized multi-enzyme system. The inventionalso discloses the immobilized multi-enzyme system prepared by the preparation method of the immobilized multi-enzyme system. According to the invention, ultraviolet irradiation is combined with themetal organic frame, operation is simple and convenient, the curing reaction condition is mild, and curing efficiency is high, so that the problems of low organic matter degradation rate, small solidload, poor stability and the like are effectively solved. The method is suitable for large-scale production, and lysis conditioning of sludge or the treatment effect of organic wastewater can be enhanced.

Description

Technical field [0001] The invention relates to the technical field of enzyme immobilization, in particular to an immobilized multi-enzyme system and a preparation method thereof. Background technique [0002] In recent years, with the development of industry and agriculture, environmental pollution has become more and more serious. Research on pollution control, especially green and energy-saving biological treatment and restoration, is in the ascendant. The application of enzymes as biocatalysts in the field of pollution control has attracted widespread attention. For example, Lysozyme (LZM) can be used for bacteriolytic conditioning of sewage sludge. BOD of some refractory wastewater 5 The / COD is low, that is, the biochemical property is low, and the conventional anaerobic and aerobic biological treatment is more difficult. The enzyme treatment of these wastewater has attracted widespread attention. For example, horseradish peroxidase (Horseradish peroxidase, HRP) and cheap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C02F3/34C02F101/30
CPCC12N11/14C02F3/342C02F2101/306
Inventor 袁世斌黄奇萍莫元渝
Owner SOUTHWEAT UNIV OF SCI & TECH
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