General macro bar code amplification primers for marine zooplanktons

Abstract

The invention provides general macro bar code amplification primers for marine zooplanktons and belongs to the technical field of molecular biologics. The primers comprise a forward primer group and areverse primer group, wherein the forward primer group consists of one or more of the following primers: a primer F1, a primer F2, a primer F3, a primer F4, a primer F5, a primer F6, a primer F7, a primer F8, a primer F9 and a primer F10; and the reverse primer group consists of one or more of the following primers: a primer R1, a primer R2, a primer R3, a primer R4, a primer R5, a primer R6, a primer R7, a primer R8, a primer R9 and a primer R10. The primers comprise bar code sequences provided by the invention, the combination performance of the primers with templates can be improved when PCR (polymerase chain reaction) amplification is implemented, the PCR efficiency can be improved, the output of sequencing sequences can be increased, different samples can be accurately distinguished,the splitting rate of the bar code sequences can be increased, the sequencing quality can be improved, and the cover degree of identified species types can be increased.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a universal macro barcode amplification primer for marine zooplankton. Background technique [0002] Zooplankton is an important part of marine biodiversity because of its many types, large numbers and wide distribution. As a key link in the transmission of marine primary productivity to high trophic levels, zooplankton play an important role in the material cycle and energy flow of marine ecosystems. At the same time, zooplankton are very sensitive to environmental disturbances. An important indicator of system structure changes can provide indicators for evaluating the environmental quality and health of marine ecosystems. Accurate identification of zooplankton species is the basis of planktonic ecology research. Traditional morphological identification methods cannot meet the automated and standardized data requirements of modern marine ecological manage...

AI Technical Summary

Problems solved by technology

Accurate identification of zooplankton species is the basis of planktonic ecology research. Traditional morphological identification methods cannot meet the automated and standardized data requirements of modern marine ecological management and protection. The emergence of new technologies such as ZooScan image recognition and DNA barcode technology has greatly promoted Advances in Zooplankton Taxonomy, Ecology and Related Fields

Zooplankton taxa are characterized by high diversity. The traditional microscopic counting method based on morphology requires high professional skills of the identification personnel, and the results of different identification personnel are not comparable, resulting in the lack of comparability of the results between different years. These defects make it difficult for morphological-based identification methods to meet the needs of operational monitoring. Therefore, it is urgent to develop automated and standardized identification technologies for zooplankton species.

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