A kind of biological deodorization preparation and its preparation method and application
A technology for biological deodorization and preparation, applied in the field of animal husbandry engineering, can solve problems such as affecting human health, susceptibility to diseases, mucosal damage, etc., and achieve the effect of obvious industrialization prospects, high cell viability, and multi-cell count.
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[0022] Example of product preparation
[0023] 1. Preparation medium
[0024] 1. Medium
[0025] 1.1 Seed medium: peptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L.
[0026] 1.2 Basic fermentation medium: disodium hydrogen phosphate 8.5g / L, potassium dihydrogen phosphate 3g / L, ammonium chloride 1g / L, sodium chloride 0.5g / L, brown sugar 50g / L, magnesium sulfate heptahydrate 0.492g / L L, where the pH value is 6.8-7.0.
[0027] 1.3 Instruments and equipment: temperature-controlled shaker, autoclave, electronic balance, sterile operating table, ultraviolet spectrophotometer
[0028]2. Preparation of Bacillus methylotrophicus (Bacillus methylotrophicus) GBacillus-9 strain fermentation broth
[0029] 2.1 Culture method
[0030] 2.1.1 Strain preservation method: Mix the bacterial solution when the OD600 of the seed medium reaches 1.5 and 50% sterile glycerol in a 1:1 ratio in a 1.5ml EP tube, seal it with a parafilm, and store it in a -20°C refrigerator, long-term storage...
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