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A kind of biological deodorization preparation and its preparation method and application

A technology for biological deodorization and preparation, applied in the field of animal husbandry engineering, can solve problems such as affecting human health, susceptibility to diseases, mucosal damage, etc., and achieve the effect of obvious industrialization prospects, high cell viability, and multi-cell count.

Active Publication Date: 2022-04-26
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These gaseous substances not only affect human health, but also are easily adsorbed on the mucous membrane of the respiratory tract, and are corrosive. In the long run, they will cause damage to the mucous membrane, resulting in poor epidemic prevention of pigs and easy to get sick
At the same time, after ammonia gas enters the body, it will hinder the absorption of nutrients, and will make the living environment of germs more comfortable, breed bacteria, and cause great losses to animal husbandry farms. Therefore, the sustainable development of animal husbandry production requires a High-efficiency, low-cost solution to reduce ammonia and other odor emissions
[0003] At present, there are some deodorization methods for farms that rely on physical adsorption and other methods, but these methods require a large cost of supporting infrastructure and are difficult to effectively promote
However, there are still few types of microbial deodorants or functional feed preparations aimed at reducing and removing the odor of farms, with narrow user options, single use methods, and unsatisfactory use effects. Therefore, it is urgent to develop a high-efficiency, low-efficiency Cost-effective, safe biological deodorizers or deodorizing feed additives reduce ammonia and odor emissions at the source

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] Example of product preparation

[0023] 1. Preparation medium

[0024] 1. Medium

[0025] 1.1 Seed medium: peptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L.

[0026] 1.2 Basic fermentation medium: disodium hydrogen phosphate 8.5g / L, potassium dihydrogen phosphate 3g / L, ammonium chloride 1g / L, sodium chloride 0.5g / L, brown sugar 50g / L, magnesium sulfate heptahydrate 0.492g / L L, where the pH value is 6.8-7.0.

[0027] 1.3 Instruments and equipment: temperature-controlled shaker, autoclave, electronic balance, sterile operating table, ultraviolet spectrophotometer

[0028]2. Preparation of Bacillus methylotrophicus (Bacillus methylotrophicus) GBacillus-9 strain fermentation broth

[0029] 2.1 Culture method

[0030] 2.1.1 Strain preservation method: Mix the bacterial solution when the OD600 of the seed medium reaches 1.5 and 50% sterile glycerol in a 1:1 ratio in a 1.5ml EP tube, seal it with a parafilm, and store it in a -20°C refrigerator, long-term storage...

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Abstract

The invention relates to a biological deodorant preparation, which is characterized in that the active ingredient is Bacillus methylotrophicus (Bacillus methylotrophicus) GBacillus‑9 strain, and the results of pasture tests show that the biological deodorization preparation of the invention can significantly reduce The ammonia concentration in pig farm sheds has a wide range of application prospects. It has a good effect on reducing the ammonia emissions of farms, especially pig farms. The raw material of the biological preparation product of the present invention is easy to obtain, the process is simple, and is suitable for large-scale production; at the same time, the biological preparation of the present invention can be used in simple ways such as spraying, adding animal drinking water and / or adding feed, which can effectively save labor costs and infrastructure cost.

Description

technical field [0001] The invention belongs to the field of animal husbandry engineering, and in particular relates to a biological deodorizing preparation suitable for livestock and poultry farms and its preparation and application. Background technique [0002] As the country continues to introduce more and more stringent policies and regulations on livestock producers, environmental protection has become the biggest production problem faced by farms, and among many farms, the environmental protection problem of pig farms is the most prominent. Gases such as ammonia and hydrogen sulfide emitted by pigs and pig manure are the main sources of odor in pig farms. Because the environment is not up to standard, the surrounding people have strong reactions, and farms have been shut down for renovation from time to time. These gaseous substances not only affect human health, but are also easily adsorbed on the mucous membrane of the respiratory tract and are corrosive. In the lon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/58A23K10/18C12R1/07
CPCC12N1/20B01D53/84B01D53/58A23K10/18B01D2257/406B01D2258/0266Y02A50/20
Inventor 石恒波林国栋吕正兵舒建洪杨一凡娄港归方银兵
Owner ZHEJIANG SCI-TECH UNIV
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