Hypoglycemic active ingredient in panax japonicus, hypoglycemic composition and application thereof
A composition and technology of ginseng saponins are applied in the field of traditional Chinese medicine, and can solve the problems of single target point, obvious toxic and side effects, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0110] 1.6.1 Preparation method of total saponins of Panax ginseng (one to five methods)
[0111] Method 1: Grind the dried bamboo ginseng medicinal material into coarse particles, weigh 10 kg, reflux extract with 70% ethanol for 3 times, each time for 2 hours, filter, combine the filtrates, and concentrate under reduced pressure to obtain the extract (take part of the dried part to obtain the total Extract 1) Add appropriate amount of water to suspend, filter, apply to D101 macroporous resin, start gradient elution from 10%, 30%, 50%, 70%, 90% ethanol, monitor the elution according to the thin-layer plate TLC color method Does the eluent contain bamboo ginsenoside V, pseudoginsenoside RT1, bamboo ginsenoside IV, bamboo ginsenoside IVa, any of the four appear in the eluent and start to collect until all four are eluted from the resin The collection was stopped, and this part of the eluate was concentrated and dried to obtain the total saponins of Panax ginseng 1.
[0112] Met...
Embodiment 1
[0124] 1. Effect of total saponins of Bamboo Ginseng on glucose consumption in HePG2 cell insulin resistance model [1]
[0125] When the cells grow to 80-90% confluence in the culture flask, discard the medium and wash it twice with PBS; digest the cells with 0.25% trypsin for about 1 min, pour off the trypsin, and add the medium containing 10% serum Stop the digestion, pour out the medium, add new medium containing 10% fetal bovine serum, blow the cells gently; count the cells, dilute the cells to 8×10 4 cells / mL, inoculated in a 96-well plate, inoculated 200 μL per well, and added 200 μL of LD-Hank’s solution to each well around it. Place the 96-well plate in 5% CO after seeding the cells 2 , Cultivate in a constant temperature incubator at 37°C, when the cells grow to 80-90% confluence, suck out the culture medium, and divide the cells into: (1) blank group 180 μL high-sugar DMEM and 20 μL 10% BSA in PBS solution; (2) Model group: 180 μL high-sugar DMEM and 20 μL 1 mmol / ...
Embodiment 2
[0139] 2. Effect of total saponins of Bamboo Ginseng on cell viability and NO secretion in high glucose-induced HUVEC injury model [2-6]
[0140] When the cells were incubated in the culture flask until they were 90% confluent, discard the medium and wash twice with PBS; digest the cells with 0.25% trypsin for about 1 min, pour off the trypsin, and add 10% serum (w / v, g / 100ml) medium to stop digestion, discard the medium, add 4mL of new medium containing 10% fetal bovine serum (w / v), blow the cells gently; perform cell counting, and dilute the cells to 3×10 4 cells / mL, inoculated in 96-well plate, inoculated 200 μL per well.
[0141] Place the 96-well plate in 5% CO 2 1. Cultivate in a constant temperature incubator at 37°C. When the cells grow to 90% confluence, replace them with low-sugar DMEM medium containing 1% fetal bovine serum and culture for 24 hours to synchronize the cells. The cells were divided into blank control group, model control group and drug administrati...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com