Complex microbial inoculant for cardiovascular diseases
A compound bacterial agent and cardiovascular technology, applied in the field of microorganisms, can solve problems such as increasing the risk of cardiovascular diseases, thrombus blockage, narrowing of arterial diameter, etc., and achieve the effects of improving immunity, improving health, and ensuring safety
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Embodiment 1
[0034] Determination of total cholesterol content in the serum of embodiment 1
[0035] 1. Activated bacteria:
[0036] Lactobacillus plantarum CGMCC №.3698: Take a ring of Lactobacillus plantarum and inoculate it into MRS solid medium for streaking, and cultivate anaerobically at 37°C for 24 hours. Pick a single colony with good growth in the plate and inoculate it into MRS liquid medium for activation, and culture it anaerobically to 0.5*10 8 cfu / ml of Lactobacillus plantarum CGMCC №.3698 seed liquid, adjusted to pH 6.0 with lactic acid.
[0037] 2. Configure liquid
[0038] Preparation of the Chiatin A solution: use water, Tween 80 (mass fraction 1%) and Chiatin A to prepare a solution with a concentration of Chiatin A of 10 mg / kg.
[0039] 3. Fermentation broth preparation
[0040] a. Lactobacillus plantarum CGMCC №.3698 fermentation liquid: Lactobacillus plantarum CGMCC №.3698 seed liquid is activated in MRS liquid medium, anaerobic culture is 24h, and the use medium ...
Embodiment 2
[0065] Embodiment 2 triglyceride determination
[0066] 1. Determination of triglyceride content in serum
[0067] Determination method: configure blank tube (distilled water 10ul, working solution 1000ul), calibration tube (2.26mmol / L calibrator 10ul, working solution 1000ul), sample tube solution (sample 10ul, working solution 1000ul). Mix well, incubate at 37°C for 10min, wavelength 510nm, optical path 0.5cm, adjust to zero with distilled water, and measure the absorbance value of each tube.
[0068] Calculation formula: Triglyceride content (nmol / L) = (sample OD value - blank OD value) / (calibration OD value - blank OD value) * calibrator concentration
[0069] According to the calculation formula, the triglyceride content of rat samples in different groups was determined.
[0070] 2. Statistical analysis was performed using SPSS16.0, and the results of measurement data were expressed as mean ± standard deviation, and the t-test between groups was used.
[0071] 3. The...
Embodiment 3
[0079] Stability test: After adding red yeast rice powder, natto freeze-dried powder, and chitosan to the Lactobacillus plantarum + Chitosin A treatment group, the lipid-lowering effect did not decrease after the test, and it could be stabilized after homogeneous emulsification Stable at room temperature for 12 months.
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