Method for identifying specific markers and difference markers of genuine medicinal materials and judging genuine medicinal materials by utilizing mass spectrometry technology

Abstract

The invention discloses a method for identifying specific markers and difference markers of genuine medicinal materials and judging genuine medicinal materials by using a mass spectrometry technology.The method comprises steps of (1) selecting genuine medicinal material and non-genuine medicinal material samples to prepare mass spectrometry to-be-detected solution; (2) collecting mass spectrum data of to-be-detected solution; (3) extracting the mass spectrum data in the step (2), generating peak intensity tables of components of the genuine and non-genuine medicinal material samples, and normalizing the obtained data; (4) screening the specific markers in the genuine medicinal materials on the basis of VIP-plot and T-inspection methods, performing MS-MS analysis, comparing the obtained MS-MS data with a database, and identifying the specific markers; and (5) repeating the steps (1) to (3) to obtain the mass spectrum data of unknown medicinal materials, and based on the specific markers in the step (4), establishing an artificial neural network or an ROC curve and other prediction models to judge whether the unknown medicinal materials are genuine medicinal materials or not.

Description

technical field [0001] The invention relates to the field of botanicals, in particular to a method for identifying specific markers of authentic medicinal materials by using mass spectrometry technology combined with chemometric pattern recognition technology to prove the authenticity of medicinal materials. Background technique [0002] Authentic medicinal materials refer to medicinal materials with excellent efficacy. This concept originated from production and clinical practice of traditional Chinese medicine. It has been confirmed by countless clinical practices of traditional Chinese medicine for thousands of years. The unique comprehensive standard is also a unique comprehensive discrimination standard for controlling the quality of medicinal materials in traditional Chinese medicine. It is popularly believed that authentic medicinal materials refer to medicinal materials produced in a specific natural condition and ecological environment, and the production is relativ...

AI Technical Summary

Problems solved by technology

[0003] Recently, scientific and technical personnel have conducted a lot of research on the authenticity of medicinal materials, using methods such as omics, fingerprints, infrared spectroscopy, electronic nose, and even genetic information research. However, the authenticity of many medicinal materials is still at a standstill. In terms of "authentic goods", even the "high quality" of traditional real estate areas has been challenged to a certain extent

[0004] Due to the lack of quantitative analysis basis for the identification of authentic medicinal materials, the pharmacological research and adulteration prevention of medicinal materials lack scientific basis

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