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Method for detecting short-chain fatty acids in serum and lymphatic tissues based on GC-MS

A technology of medium and short-chain fatty acids and lymphoid tissue, applied in the field of analytical chemistry, can solve the problems of cumbersome operation steps, low recovery results, high time cost, etc., and achieve the effect of good repeatability and high recovery rate

Active Publication Date: 2020-06-05
SHANDONG SHANWEI IMMUNOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The inventors found that there is currently no report on the quantitative detection method of short-chain fatty acids in lymphoid tissue. Although the quantitative method of short-chain fatty acids in serum has been reported, due to its cumbersome operation steps, high time cost, and low recovery results, etc. Features are not widely used

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  • Method for detecting short-chain fatty acids in serum and lymphatic tissues based on GC-MS
  • Method for detecting short-chain fatty acids in serum and lymphatic tissues based on GC-MS
  • Method for detecting short-chain fatty acids in serum and lymphatic tissues based on GC-MS

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Embodiment 1

[0040] (1) Instruments and reagents

[0041] Agilent 7890A gas chromatograph (Agilent Technology, USA); IKA VORTEX 3 vortex mixer (IKA, Germany); HH-S constant temperature water bath (Jintan Medical Instrument Factory, China); refrigerated centrifuge (Thermo Fisher, USA) ); Milli-Q ultrapure water system (Millipore, USA).

[0042] Acetic acid, propionic acid, butyric acid, 2-ethylbutyric acid, pentafluorobromide (Sigma-Aldrich, USA); acetone, n-hexane (China Kemiou Reagent Company); phosphate buffer (China Dingguo Bioreagents) company);

[0043] (2) Sample pretreatment

[0044] Serum: Let the collected whole blood stand at room temperature for 30 minutes, centrifuge at 2000 rpm at 4°C for 20 minutes, and take the supernatant;

[0045] Lymphatic tissue: Accurately weigh 50mg of lymphoid tissue, add 1mL of pre-cooled ultrapure water, use a tissue grinder to grind evenly at 4°C, vortex vigorously for 1min, extract ultrasonically for 15min, centrifuge at 10,000rpm at 4°C for 10...

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Abstract

The invention discloses a method for detecting short-chain fatty acids in serum and lymphatic tissues based on GC-MS. The method comprises the following steps: pretreating lymphatic tissues to obtaina lymphatic extracting solution; adding an internal standard substance into the serum or lymph extracting solution, and adding benzyl pentafluorobromide for derivatization reaction; and extracting thederived solution, and detecting acetic acid, propionic acid and butyric acid in the extract by using GC-MS. A gas chromatography-mass spectrometry rapid determination method for detecting three short-chain fatty acids in the serum and lymphatic tissues is established through a derivatization method. Different biological samples are pretreated and subjected to derivatization treatment, the internal standard is added for correction and accurate quantification is achieved.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for detecting short-chain fatty acids in serum and lymphoid tissue based on GC-MS. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Short chain fatty acids (Short chain fatty acids), also known as volatile fatty acids, according to the number of carbon atoms, organic fatty acids with a carbon number of 1-6 are called short chain fatty acids, of which acetic acid, propionic acid and butyric acid are due to Its potential biological effects have attracted much attention. The high concentration of short-chain fatty acids in the intestine can regulate the immu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045G01N2030/067
Inventor 宋月曲新艳王泉博
Owner SHANDONG SHANWEI IMMUNOTECH CO LTD
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