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Cell membrane coated ultra-small ferroferric oxide nanocluster, and preparation and application thereof

A technology of ultra-small ferroferric oxide and ferroferric oxide, which is applied in the field of nanomedical diagnosis and treatment materials and its preparation and application, and achieves the effect of good pH response performance

Active Publication Date: 2020-06-05
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Searching domestic and foreign literature and patents has not found anything about the preparation of ultra-small ferric oxide nanoclusters coated with cell membranes and pH-responsive properties, and the anticancer drug doxorubicin (DOX) loaded in its cavity and combined with ultrasound target Microbubble Destruction Technology (UTMD) for in vivo T 1 / T 2 Related reports on dual-modality MR imaging and chemotherapy

Method used

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  • Cell membrane coated ultra-small ferroferric oxide nanocluster, and preparation and application thereof
  • Cell membrane coated ultra-small ferroferric oxide nanocluster, and preparation and application thereof
  • Cell membrane coated ultra-small ferroferric oxide nanocluster, and preparation and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] (1) Dissolve 0.6488g of ferric chloride in 40mL of diethylene glycol (also known as diethylene glycol, DEG), and then 0.47g of sodium citrate (Na 3 Cit) was dissolved in the above solution, and stirred at 80° C. for 1 h under an air atmosphere. After the sodium citrate was completely dissolved, 1.312 g of anhydrous sodium acetate was added to the above solution, and the stirring was continued until the sodium acetate powder was completely dissolved. Then transfer the solution to a 50mL autoclave and react at 200°C for 4h; Redissolve in pure water, centrifuge at 8500rpm for 15min, repeat the operation 3 times, and then dry the precipitate in a vacuum oven at 60°C to obtain ultra-small Fe stabilized by sodium citrate on the surface 3 o 4 Nanoparticles (USIONP S ).

[0086] (2) Take 56mg USIONP S Dissolve in 16mL ultrapure water, keep stirring, dropwise add EDC (150mg, 1mL ultrapure water) to the solution, after 30min, add dropwise NHS (95mg, 1mL ultrapure water) to th...

Embodiment 2

[0092] Get the USIONP synthesized in Example 1 S 、IONC S 、IONC S / DOX, IONC S / DOX@CMM each 1mg, dilute to 50μg / mL with ultrapure water, and take 50μL of USIONP at the same time S -NH 2 Dissolved in 950 μL of ultrapure water for the determination of surface potential and hydrodynamic diameter. As shown in Table 1, USIONP S The potential of -40.3mV, USIONP S -NH 2 The potential of -17.2mV, the potential rise, proved the successful modification of ethylenediamine (EDA). Coupling USIONP with terephthalaldehyde S -NH 2 , the potential changed to -14.6mV, and the hydrated particle size increased to 372.6nm, which proved that the ferric oxide nanoclusters (IONC S ) was successfully synthesized. When IONC S After loading the chemotherapeutic drug DOX, the potential changed from negative to positive, and the hydrated particle size increased to 746.2nm, which proved the successful loading of DOX. By physical extrusion in IONC S After the surface of / DOX is coated with the...

Embodiment 3

[0097] Get the USIONP prepared in Example 1 S 、USIONP S -NH 2 、IONC S Perform FT-IR characterization, such as image 3 As shown, 3400-3500cm in curve a -1 Nearby is the stretching vibration absorption peak of free hydroxyl (-OH), 2882-2987cm -1 The nearby characteristic absorption peak is attributed to the stretching vibration absorption peak of methylene in sodium citrate, 1488-1718cm -1 The nearby characteristic absorption peak is attributed to the stretching vibration absorption peak of carbonyl group (C=O). 1546cm in curve b -1 Nearby is the stretching vibration absorption peak of the carbon-nitrogen bond (C-N), which is the same as the 1598cm -1 The stretching vibration absorption peaks of the carbonyl group (C=O) partially overlap, which proves that ethylenediamine (EDA) is in the ultra-small iron ferric oxide nanoparticles (USIONP S ) surface for successful modification. 1573-1724cm in curve c -1 Nearby is the characteristic absorption peak of the imine bond (...

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Abstract

The invention relates to a cell membrane coated ultra-small ferroferric oxide nanocluster, and a preparation method and an application thereof. The method comprises the following steps: preparation ofultra-small ferroferric oxide nanoparticles, preparation of surface aminated ultra-small ferroferric oxide nanoparticles, preparation of ferroferric oxide nanoclusters, preparation of adriamycin-loaded ferroferric oxide nanoclusters, preparation of a cell suspension, and preparation of cell membrane coated ultra-small ferroferric oxide nanoclusters. After the nanocluster provided by the inventionis injected into a mouse body through a tail vein, T1 / T2 bimodal MR imaging conversion can be achieved; meanwhile, the anti-tumor effect can be exerted; the chemotherapy effect of the nanocluster canbe further enhanced through a UTMD auxiliary means; and the nanocluster has potential clinical application value.

Description

technical field [0001] The invention belongs to the field of nano-medicine diagnosis and treatment materials and their preparation and application, in particular to a cell membrane-coated ultra-small iron ferric oxide nano-cluster and its preparation method and application. Background technique [0002] Nowadays, cancer has become one of the main factors threatening human health. At present, although many chemotherapeutic drugs in clinical use can inhibit the growth of tumors to a certain extent, they have the disadvantages of large toxic and side effects, low utilization rate of drugs, and large cumulative dosage. However, nano-platforms constructed by loading small-molecule anti-cancer drugs on nanomaterials have the advantages of targeted drug delivery and high drug release at the lesion site, which can reduce normal tissue damage and improve therapeutic efficacy. Compared with numerous nanocarriers, the ultra-small Fe3O4 nanoparticles (USIONP S ) has the characteristic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K9/50A61K31/704A61K47/46A61K47/02A61P35/00
CPCA61K9/5068A61K31/704A61K47/02A61K49/1896A61P35/00
Inventor 沈明武贾亮史向阳
Owner DONGHUA UNIV
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