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Method for improving gastric cancer by TIL immune cells

A technology of immune cells and gastric cancer, applied in the field of immune cell therapy, can solve the problems of limited types and effects of drugs, and achieve the effect of high killing ability, low side effects, and high side effects

Inactive Publication Date: 2020-06-05
卡替(上海)细胞生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, the types and functions of targeted therapy drugs for gastric cancer are limited

Method used

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  • Method for improving gastric cancer by TIL immune cells

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Embodiment Construction

[0024] The present invention will be described in detail below in conjunction with specific embodiments.

[0025] The invention discloses a method for improving gastric cancer by using TIL immune cells. The specific operation process is as follows: (1) first remove the tumor from the patient, transport the tumor tissue to a GMP laboratory under sterile and cold chain conditions, and separate get 10 4 ~10 6 (2) The initial tumor-infiltrating lymphocytes were cultured under suitable conditions, amplified 10,000 times and further purified to reach 10 8 The primary seed cells were then cryopreserved, and the cryopreservation conditions were 10 tubes per group, 10 7 cells / branch, stored at -196°C in liquid nitrogen; (3) Tumor-infiltrating lymphocytes were recovered and expanded to 8×10 7 (4) Tumor infiltrating lymphocytes were further expanded to 1,000 to 10,000 times, and tumor infiltrating lymphocytes were further expanded to 10 10 (5) Finally, the tumor-infiltrating lymphocy...

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Abstract

The invention discloses a method for improving gastric cancer through TIL immune cells, which comprises the following steps of: firstly, cutting off tumors from the body of a patient, transporting tumor tissues to a GMP laboratory under sterile and cold-chain conditions, and separating to obtain initial tumor infiltrating lymphocytes; culturing the initial tumor infiltrating lymphocytes under proper conditions, amplifying by 10,000 times, further purifying, and then cryopreserving; resuscitating the tumor infiltrating lymphocytes, amplifying the tumor infiltrating lymphocytes to 8 * 10 < 7 >,and knocking down PD-1 by using a slow virus vector RNAi technology; further amplifying the number of the tumor infiltrating lymphocytes to 10 <10> or more; finally, infusing back the tumor infiltrating lymphocytes to the patient, and the average cell quantity of the tumor infiltrating lymphocytes injected each time is 7.3 * 10 < 8 >. The invention has efficient killing ability, can identify a plurality of antigen targets, uses autoimmune cells, and has no side effect, and the TIL immunotherapy can be used for patients with recurrent or metastatic advanced gastric cancer.

Description

technical field [0001] The invention belongs to the technical field of immune cell therapy, and in particular relates to a method for improving gastric cancer through TIL immune cells. Background technique [0002] Gastric cancer (gastric carcinoma) is a malignant tumor originating from the gastric mucosal epithelium. It ranks first in the incidence of various malignant tumors in my country. The incidence of gastric cancer has obvious regional differences. The incidence of gastric cancer in the northwest and eastern coastal areas of my country is higher than that in the south. area is significantly higher. The age of onset is more than 50 years old, and the ratio of male to female incidence is 2:1. Due to changes in diet structure, increased work pressure, and Helicobacter pylori infection, gastric cancer tends to be younger. Gastric cancer can occur in any part of the stomach, and more than half of them occur in the gastric antrum, greater curvature, lesser curvature, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K35/17A61P35/00C12N5/10
CPCA61K35/17A61K39/0011A61P35/00C07K14/70521C12N5/0638C12N2510/00
Inventor 李军
Owner 卡替(上海)细胞生物技术有限公司
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