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HLA (Human Leukocyte Antigen)-A*0201 limitation CTL (Cytotoxic T Lymphocyte) epitope of zinc translocator and application thereof

A technology of HLA-A and zinc transporter, which is applied in the application field of the CTL epitope, to achieve good development and application prospects and high-efficiency killing ability

Inactive Publication Date: 2012-11-21
ARMY MEDICAL UNIV
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Problems solved by technology

[0005] As far as the inventors know, the HLA-A of ZnT8 * 0201 restricted CTL epitope has not been reported at home and abroad

Method used

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  • HLA (Human Leukocyte Antigen)-A*0201 limitation CTL (Cytotoxic T Lymphocyte) epitope of zinc translocator and application thereof
  • HLA (Human Leukocyte Antigen)-A*0201 limitation CTL (Cytotoxic T Lymphocyte) epitope of zinc translocator and application thereof
  • HLA (Human Leukocyte Antigen)-A*0201 limitation CTL (Cytotoxic T Lymphocyte) epitope of zinc translocator and application thereof

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Embodiment Construction

[0016] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0017] 1. Prediction of HLA-A of ZnT8 ＊ 0201 restricted CTL epitope

[0018] According to the amino acid sequence of human ZnT8 registered in Genbank (accession number is AAH28419), two different methods are combined: the method based on the binding strength of peptides to MHC-I molecules (including the two algorithms of BIMAS and SYFPEITHI) and the method based on proteasome cleavage specificity method (PAProc algorithm) to predict HLA-A of ZnT8 * 0201 restricted CTL epitope. Both algorithms, BIMAS and SYFPEITHI, are based on quantitative matrices to predict the binding strength of peptides to MHC-I molecules. The PAProc algorithm is based on a single-layer neural network algorithm and proteasome cleavage data from in vitro experiments. Its biggest advantage ...

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Abstract

The invention belongs to the field of biomedicine, in particular to an HLA (Human Leukocyte Antigen)-A*0201 limitation CTL (Cytotoxic T Lymphocyte) epitope of a zinc translocator and application thereof. The amino acid sequence of the CTL epitope is shown as the SEQ ID No.1, the SEQ ID No.2 or the SEQ ID No.3, the CTL epitope can be effectively combined with HLA-A*0201 molecules and has high affinity with the HLA-A*0201 molecules, the formed compound is stable and can effectively excite CTL response with specificity, secrete high-level IFN (Interferon)-gamma, has the capacity of efficiently killing T2 cells in the relevant epitopes of a load, can be used for preparing peptide vaccines for treating I type diabetes mellitus, and has potential and favorable development and application prospect in the field of clinical immunization therapy on the I type diabetes mellitus.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a cytotoxic T cell (CTL) epitope of an antigen protein, in particular to HLA-A of the main autoantigen zinc transporter 8 (ZnT8) of type 1 diabetes (T1DM) * The 0201 restricted CTL epitope also relates to the application of the CTL epitope. Background technique [0002] T1DM is an autoimmune disease mediated by lymphocytes, characterized by the absolute deficiency of insulin production due to the specific damage of pancreatic β cells. It mostly occurs in children and adolescents. Severe complications of important tissues and organs such as liver, kidney, nerves, and eyes are one of the main causes of disability and premature death in children and adolescents. In recent years, the number of children and adolescents in my country suffering from T1DM has been increasing year by year, and T1DM is becoming a major disease that endangers the health of children and adolescents in my country. C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K38/08A61P3/10
Inventor 杨曌吴玉章
Owner ARMY MEDICAL UNIV
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