A preparation method of Saccharomyces cerevisiae and isomerized lactose powder
A technology of Saccharomyces cerevisiae and isomerization, which is applied in the field of preparation of isomerized lactose powder, can solve the problems of high loss rate of sugar liquid, difficult crystallization and precipitation, and low galactose removal rate, and achieves the effect of high yield
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Embodiment 1
[0027] Prepare a lactose solution with a concentration of 50%, add an isomerizing agent sodium carbonate with 5% lactose mass, react at 110°C for 2 hours, then cool to crystallize the lactose, and centrifuge to obtain a centrifuged mother liquor. The purity of lactose is 25.57%, and the purity of isomerized lactose is The purity of galactose is 58.45%, 14.18%, and the concentration is 28% after ion exchange.
[0028] Add Saccharomyces cerevisiae LJSM-01 with a quality of 1% of the volume of the centrifuged mother liquor (this strain was preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on November 29, 2019, with a preservation number of CGMCC No.19055, address: Beijing The Saccharomyces cerevisiae LJSM-01 was collected from an environment rich in galactose, and a strain obtained by mutagenesis screening could completely metabolize galactose into Carbon dioxide and water, but strains that cannot metabolize lactulose.
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Embodiment 2
[0031] Prepare a lactose solution with a concentration of 60%, add isomer sodium hydroxide with a lactose mass of 0.5%, react at 100°C for 0.5h, then cool to crystallize lactose, centrifuge to obtain a centrifuged mother liquor, which is composed of lactose with a purity of 29.86%, isomerized lactose Purity is 55.32%, galactose purity is 12.31%, it is through ion exchange, and concentration is 30%, to wherein adding quality is the saccharomyces cerevisiae LJSM-01 (same embodiment 1) of centrifugal mother liquor volume 0.5%, fermentation condition is: The initial pH value is 6.47, the temperature is 28°C, and the rotation speed is 600r / min. After 20 hours of fermentation, the galactose is completely metabolized. After sterilization, decolorization, ion exchange and evaporation to a concentration of 40%, then simulated moving bed chromatography purification, the purification temperature is 60 ° C, after purification, the concentration of the material rich in isomerized lactose is...
Embodiment 3
[0033]Prepare a lactose solution with a concentration of 40%, add an isomer sodium dihydrogen phosphate with 8% lactose mass, react at 120°C for 3 hours, then cool to crystallize lactose, and centrifuge to obtain a centrifuged mother liquor, which is composed of lactose with a purity of 21.35% and isomerized lactose Purity is 60.43%, galactose purity is 16.56%, it is through ion exchange, and concentration is 20%, to wherein adding quality is the saccharomyces cerevisiae LJSM-01 (same embodiment 1) of centrifugal mother liquor volume 2%, fermentation condition is: The initial pH value is 3.68, the temperature is 35°C, and the rotation speed is 100r / min. After 33 hours of fermentation, the galactose is completely metabolized. After sterilization, decolorization, ion exchange and evaporation to a concentration of 60%, then simulated moving bed chromatography purification, the purification temperature is 50 ° C, after purification, the concentration of the material rich in isomeri...
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