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A preparation method of Saccharomyces cerevisiae and isomerized lactose powder

A technology of Saccharomyces cerevisiae and isomerization, which is applied in the field of preparation of isomerized lactose powder, can solve the problems of high loss rate of sugar liquid, difficult crystallization and precipitation, and low galactose removal rate, and achieves the effect of high yield

Active Publication Date: 2022-03-08
山东绿健生命生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the isomerized lactose products on the market are mainly liquid. Whether it is the preparation of isomerized lactose by boric acid method or other chemical reagents, there will be by-product galactose and unconverted lactose. Structured lactose is easily soluble in water and is not easy to crystallize out. In order to obtain isomerized lactose powder, galactose and lactose need to be removed. The existing technology is mainly through simulated moving bed chromatography purification or nanofiltration membrane method. Lactose or the removal of lactose, such as CN201110046277.4, CN201710656155.4 and CN201611103063.5, due to the existence of two main miscellaneous sugars, and the separation degree of the adsorbent for isomerized lactose and the two miscellaneous sugars is small, and a The retention time of the heterosaccharide is before the isomerized lactose, and the retention time of another heterosaccharide is after the isomerized lactose, which makes it very difficult to purify the isomerized lactose by simulated moving bed chromatography, even for the advanced three groups It is also difficult to improve the isomerized lactose to the ideal purity by the sequential simulated moving bed chromatography purification method, and at the same time achieve a higher yield; the nanofiltration membrane method cannot remove the lactose, and the removal rate of the galactose is very low , and the sugar solution loss rate is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Prepare a lactose solution with a concentration of 50%, add an isomerizing agent sodium carbonate with 5% lactose mass, react at 110°C for 2 hours, then cool to crystallize the lactose, and centrifuge to obtain a centrifuged mother liquor. The purity of lactose is 25.57%, and the purity of isomerized lactose is The purity of galactose is 58.45%, 14.18%, and the concentration is 28% after ion exchange.

[0028] Add Saccharomyces cerevisiae LJSM-01 with a quality of 1% of the volume of the centrifuged mother liquor (this strain was preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on November 29, 2019, with a preservation number of CGMCC No.19055, address: Beijing The Saccharomyces cerevisiae LJSM-01 was collected from an environment rich in galactose, and a strain obtained by mutagenesis screening could completely metabolize galactose into Carbon dioxide and water, but strains that cannot metabolize lactulose.

...

Embodiment 2

[0031] Prepare a lactose solution with a concentration of 60%, add isomer sodium hydroxide with a lactose mass of 0.5%, react at 100°C for 0.5h, then cool to crystallize lactose, centrifuge to obtain a centrifuged mother liquor, which is composed of lactose with a purity of 29.86%, isomerized lactose Purity is 55.32%, galactose purity is 12.31%, it is through ion exchange, and concentration is 30%, to wherein adding quality is the saccharomyces cerevisiae LJSM-01 (same embodiment 1) of centrifugal mother liquor volume 0.5%, fermentation condition is: The initial pH value is 6.47, the temperature is 28°C, and the rotation speed is 600r / min. After 20 hours of fermentation, the galactose is completely metabolized. After sterilization, decolorization, ion exchange and evaporation to a concentration of 40%, then simulated moving bed chromatography purification, the purification temperature is 60 ° C, after purification, the concentration of the material rich in isomerized lactose is...

Embodiment 3

[0033]Prepare a lactose solution with a concentration of 40%, add an isomer sodium dihydrogen phosphate with 8% lactose mass, react at 120°C for 3 hours, then cool to crystallize lactose, and centrifuge to obtain a centrifuged mother liquor, which is composed of lactose with a purity of 21.35% and isomerized lactose Purity is 60.43%, galactose purity is 16.56%, it is through ion exchange, and concentration is 20%, to wherein adding quality is the saccharomyces cerevisiae LJSM-01 (same embodiment 1) of centrifugal mother liquor volume 2%, fermentation condition is: The initial pH value is 3.68, the temperature is 35°C, and the rotation speed is 100r / min. After 33 hours of fermentation, the galactose is completely metabolized. After sterilization, decolorization, ion exchange and evaporation to a concentration of 60%, then simulated moving bed chromatography purification, the purification temperature is 50 ° C, after purification, the concentration of the material rich in isomeri...

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Abstract

The invention discloses a preparation method of Saccharomyces cerevisiae and isomerized lactose powder, which comprises the following steps: adding Saccharomyces cerevisiae LJSM‑01 to a lactose isomerization solution, carrying out fermentation, and removing galactose in the solution; removing galactose The fermented lactose liquid is subjected to sterilizing, decolorizing, ion exchange and evaporative concentration, followed by simulated moving bed chromatographic purification, and the obtained isomerized lactose solution is concentrated and crystallized to obtain isomerized lactose powder. The Saccharomyces cerevisiae can ferment all the galactose in it without fermenting the isomerized lactose. It adopts the two-step purification method of fermentation method and simulated moving bed chromatography separation method, and the obtained isomerized lactose liquid has high purity, The advantage of high yield meets the requirements of industrial production.

Description

technical field [0001] The technical field of preparation of isomerized lactose powder of the present invention, specifically relates to a preparation method of Saccharomyces cerevisiae and isomerized lactose powder. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Isomerized lactose is a disaccharide linked by β-(1→4) glycosidic bonds between galactose and fructose, also known as lactulose, which is produced by the isomerization reaction of lactose after heat treatment under alkaline conditions. have to. Isomerized lactose is a highly safe and easy-to-use functional disaccharide prebiotic. Currently, it mainly exists in two physical states, namely isomerized lactose liqu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P19/12C07H3/04C07H1/06C12R1/865
CPCC12P19/12C07H3/04C07H1/06C12R2001/865C12N1/185
Inventor 信成夫任尚美景文利于丽冯猛刘建于艳丽
Owner 山东绿健生命生物科技有限公司
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