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High-low-temperature continuous stress microalgae culture method

A microalgae culture, high and low temperature technology, applied in the direction of using microorganisms, microorganisms-based methods, biochemical equipment and methods, etc., can solve the problems of damage repair active substances, etc., to increase production, shorten the culture period, and reduce the number of cells Mortality Effect

Pending Publication Date: 2020-05-22
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no method for cultivating microalgae under high and low temperature continuous stress, so as to make them produce and accumulate damage repairing active substances in large quantities.

Method used

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  • High-low-temperature continuous stress microalgae culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Using Chlamydomonas cells (sp.cw15, purchased from the Algae Bank of Duke University in the United States) as the algae species to carry out a high and low temperature continuous stress microalgae culture, the specific steps are as follows:

[0026] 1. Obtain the pre-culture medium of Chlamydomonas cells

[0027] Prepare 12L of TAP medium, and the specific formula is shown in Table 1 below. Insert Chlamydomonas cells into it after sterilization, place the inoculated culture solution at 25°C, and the intensity of sunlight light is 100 μmol / (m 2 s) in a shaker light incubator with a rotating speed of 20 rpm, and culture for 7 days to obtain a Chlamydomonas cell preculture solution.

[0028] Table 1 TAP medium formula

[0029] compound Final concentration NH 4 Cl

7.0mmol / l CaCl 2 2H 2 o

0.45mmol / l MgSO 4 ·7H 2 o

0.83mmol / l K 2 HPO 4 (anhydrous)

1.65mmol / l K H 2 PO 4 (anhydrous)

1.05mmol / l h 2...

Embodiment 2

[0042] Chlorella (sp.dh8248, preserved in the laboratory, collected in East Lake, Wuhan) cells were used as algae species to carry out a high and low temperature continuous stress microalgae culture, the specific steps are as follows:

[0043] 1. Obtain the pre-culture solution of Chlorella cells

[0044] Prepare 12L of BG11 medium, the specific formula is shown in Table 3 below. After sterilization, insert Chlorella cells into it, place the inoculated culture solution at 25°C, and the intensity of sunlight light is 100 μmol / (m 2 s) in a shaker light incubator with a rotating speed of 20 rpm, and culture for 7 days to obtain a Chlamydomonas cell preculture solution.

[0045] Table 3 BG11 medium formula

[0046] compound Final concentration NaNO 3

1.5g / L K 2 HPO 4

0.04g / L MgSO 4 ·7H 2 o

0.075g / L CaCl 2 2H 2 o

0.036g / L Na 2 CO 3

0.02g / L citric acid 0.006g / L ammonium ferric citrate 0.006g / L...

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Abstract

The invention discloses a high-low-temperature continuous stress microalgae culture method. According to the culture method, two-stage culture is carried out, wherein in a pre-culture stage, microalgae cells are cultured to a concentration range of 1.0*10<6> / L-1.2*10<6> / L under an optimal condition of 25 DEG C and 100 [mu]mol / (m<2>.s), and then a microalgae pre-culture solution is used for continuous high-low-temperature stress. The high-low-temperature continuous stress microalgae culture method can greatly shorten the culture period, reduce the cell death rate, and obviously improve the yield of damage repair active substances such as superoxide dismutase, catalase, polyunsaturated fatty acids (PUFAs) and the like.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation, and in particular relates to a method for cultivating microalgae under high and low temperature continuous stress. Background technique [0002] Microalgae is a general term for a group of small algae in algae, which can be divided into prokaryotic algae and eukaryotic algae according to different cell types. As a pioneer species on the earth, microalgae is an autotrophic organism that uses light energy, carbon dioxide and water to synthesize various organic substances in its cells, and has a strong ability to adapt to the environment. This extremely strong adaptability is closely related to the efficient metabolic pathways formed by microalgae cells during the long-term evolution process and the large amount of various damage repair active substances produced and accumulated. [0003] Temperature is an important environmental factor that affects the growth of microalgae. It mainl...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/12C12R1/89
CPCC12N1/36C12N1/12
Inventor 高翔李登峰周维成王尧尧戴文芳
Owner NINGBO UNIV
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