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Separation process of natural free radical scavenger in Saxifraga tangutica and application of natural free radical scavenger

A Tanggut tiger ear and free radical technology, which is used in the separation/purification, application, and medical preparations containing active ingredients of carboxylic acid compounds, etc. See literature reports and other issues to achieve the effect of being suitable for large-scale production, easy to prepare materials in batches, and easy to scale

Active Publication Date: 2020-05-22
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no literature reports on the separation, preparation and application of natural free radical scavengers in Saxifrage tangutica, and the existing research has not been systematic.

Method used

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  • Separation process of natural free radical scavenger in Saxifraga tangutica and application of natural free radical scavenger
  • Separation process of natural free radical scavenger in Saxifraga tangutica and application of natural free radical scavenger
  • Separation process of natural free radical scavenger in Saxifraga tangutica and application of natural free radical scavenger

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Effect test

Embodiment 1

[0053] The separation process of natural free radical scavenger in saxifrage tangutata, the method specifically comprises the following steps:

[0054] Step 1, extraction: Take 500g of the whole herb of Saxifrage tanguticae and dry it in the shade. After coarse crushing, extract with methanol at a material-to-liquid ratio of 1g: 5mL, extract at room temperature 4 times, each time for 2 hours, filter and combine the filtrates, namely filtrate A , the filtrate A is mixed according to the amount of polyamide: the amount of saxifrage medicinal material = 1:5 and then dried under reduced pressure. Ear grass extract mixed sample 160.2g;

[0055] Step 2, coarse separation of microporous resin column: sample mixed with saxifrage extract of Tangutia tanguticae, the sample is separated by a medium-pressure chromatographic column equipped with microporous resin, detected by an ultraviolet detector with a detection wavelength of 254 nm, collected and prepared The first major peak fractio...

Embodiment 2

[0062] The separation process of natural free radical scavenger in saxifrage tangutata, the method specifically comprises the following steps:

[0063] Step 1, extraction: take 1000g of the whole herb of Saxifraga saxifrage, dry it in the shade, coarsely crush it, extract it according to the ratio of solid to liquid: 1g: 100mL of methanol, extract twice at room temperature, each time for 4 hours, filter and combine the filtrates, namely filtrate A , the filtrate A is mixed according to the amount of polyamide: the amount of saxifrage medicinal material of Tangutia saxifrage = 1:5 and then dried under reduced pressure. Ear grass extract mixed sample 719.3g;

[0064]Step 2, coarse separation of microporous resin column: sample mixed with saxifrage extract of Tangutia tanguticae, the sample is separated by a medium-pressure chromatographic column equipped with microporous resin, detected by an ultraviolet detector with a detection wavelength of 254 nm, collected and prepared The...

Embodiment 3

[0071] The separation process of natural free radical scavenger in saxifrage tangutata, the method specifically comprises the following steps:

[0072] Step 1, extraction: take 1200g of the whole saxifrage saxifrage herb, dry it in the shade, coarsely crush it, extract it with methanol at a solid-to-liquid ratio of 1g: 100mL, extract twice at room temperature, 4 hours each time, filter and combine the filtrates, namely filtrate A , the filtrate A is mixed according to the amount of polyamide: the amount of saxifrage medicinal material = 1:5 and then dried under reduced pressure. Ear grass extract mixed sample 863.7g;

[0073] Step 2, coarse separation of microporous resin column: sample mixed with saxifrage extract of Tangutia tanguticae, the sample is separated by a medium-pressure chromatographic column equipped with microporous resin, detected by an ultraviolet detector with a detection wavelength of 254 nm, collected and prepared The first main chromatographic peak fracti...

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Abstract

The invention relates to the technical field of separation of natural free radical scavengers in Saxifraga tangutica, in particular to a separation process of a natural free radical scavenger in Saxifraga tangutica and application of the natural free radical scavenger. The separation process comprises the following six steps: extraction, coarse separation via a microporous resin column, online free radical scavenger component screening, preparation via a reversed-phase preparative column, reversed-phase preparative liquid chromatographic purification of Fr1-1and hydrophilic / reversed-phase two-dimensional liquid chromatographic purification of Fr1-3. The method is low in cost, and product purity is greater than 95%; a technical means adopted by the invention can realize large-scale production: requirements on raw materials are not high, cost is low, and batch preparation is easy; room-temperature cold leaching extraction with methanol is adopted, so operation is easy; the microporous resin column is adopted for coarse separation, and a microporous resin separation material can be loaded in a medium-pressure column chromatography system, so large-scale production can be easily realized; and reversed-phase preparative liquid chromatography or hydrophilic preparative liquid chromatography used in separation and purification is a rapid isocratic method.

Description

technical field [0001] The invention relates to the technical field of separation of natural free radical scavenger in saxifrage tangutica, in particular to a separation process and application of the natural free radical scavenger in saxifrage tangutica. Background technique [0002] Tangut saxifrage ( Saxifraga tangutica Engl. ), also known as Ganqing saxifrage, is a member of the saxifrage family ( Saxifragaceae ) Saxifraga ( Saxifraga ) annual evergreen herb, Tibetan medicine name: "Songjidi", traditional Chinese medicine called "Dieda", mainly distributed in Qinghai, Gansu, Tibet, Sichuan, and Bhutan and Kashmir at an altitude of 2900~4900 meters Under coniferous thickets. Tangut saxifrage is a commonly used Tibetan medicine, and the whole herb can be used as medicine. "Chinese Medicine Cihai" records: its nature and taste are slightly bitter, pungent, and cold. Main effects: clearing the liver and gallbladder, invigorating the spleen and strengthening the stomach...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/47C07C65/03C07C67/56C07C69/84C07H15/203C07H1/08A61K31/192A61K31/235A61K31/7034A61P39/06A23L33/105
CPCA23V2002/00A61K31/192A61K31/235A61K31/7034A23L33/105A61P39/06C07C51/47C07C67/56C07H1/08C07H15/203C07C2601/16C07C65/03C07C69/84A23V2200/30
Inventor 党军陶燕铎王启兰邵赟
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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