Virus sample preservation solution, nucleic acid extraction reagent and viral nucleic acid extraction method

Abstract

The invention discloses a virus sample preservation solution with a lysis function and a method for extracting viral nucleic acid. Since sample preservation and lysis are completed in one step, no additional lysis solution is needed, further diluting of a virus sample is prevented, the adding amount of the sample during the extraction process of viral nucleic acid can be increased, and the sensitivity of subsequent detection is improved. Meanwhile, because the preservation and lysis of the virus sample are completed in one step, the step of nucleic acid detection is removed, the process is optimized, and the efficiency of sample detection is improved. Compared with a conventional virus sample preservation solution such as a PBS buffer solution, the virus sample preservation solution has good preservation effect, more detection samples can be obtained, the detection sensitivity is improved, and the processing flow is simplified.

Description

technical field [0001] The invention relates to molecular biology detection technology, in particular to a virus sample preservation solution, a virus nucleic acid extraction reagent and a virus nucleic acid extraction method. Background technique [0002] There should be normal oral flora in the isthmus of normal people without the growth of pathogenic bacteria. The bacteria in the pharynx all come from the outside world and do not cause disease under normal circumstances, but infections can occur when the body's systemic or local resistance decreases and other external factors cause diseases. Therefore, the isolation of pathogenic bacteria from pharyngeal swabs is helpful for the diagnosis of diphtheria, suppurative tonsillitis, acute pharyngitis, respiratory tract infection and other diseases. [0003] The virus sample preservation solution currently on the market can only protect the stability of the virus. However, when extracting viral nucleic acid, especially for hig...

AI Technical Summary

Problems solved by technology

There are many factors affecting the detection of viral nucleic acid, for example: (1) the content of viral nucleic acid in the sample is limited; (2) the virus sample preservation solution needs to be mixed with the lysate during the subsequent extraction of viral nucleic acid, which further reduces the concentration of viral nucleic acid; (3) The volume of the orifice plate matched with the automatic nucleic acid extraction instrument is limited, and it is impossible to increase the total amount of nucleic acid obtained by increasing the sample amount

The existence of these factors may lead to subsequent detection (such as real-time fluorescent quantitative PCR, NGS, etc.) detection failure caused by low nucleic acid input amount

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