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Skin repairing liquid and preparation method thereof

A technology for skin repair and culture fluid, applied in skin diseases, pharmaceutical formulations, animal cells, etc., to achieve the effects of easy use, easy production and manufacturing, easy packaging and transportation

Inactive Publication Date: 2020-05-19
YANAN HOSPITAL OF KUNMING CITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that there are great limitations in the direct application of stem cells to the treatment of skin wounds

Method used

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  • Skin repairing liquid and preparation method thereof
  • Skin repairing liquid and preparation method thereof
  • Skin repairing liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of skin repair liquid, specifically comprising the following steps:

[0025] (1) Cultivate human umbilical cord mesenchymal stem cells in a T-175 culture flask to make the density reach 93%, wherein the medium is α-MEM medium, the culture conditions are: temperature 37°C, carbon dioxide concentration 5%, saturated humidity.

[0026] (2) After washing and adding new α-MEM medium (the amount of α-MEM medium added is 45ml), continue to cultivate in an electric constant temperature carbon dioxide incubator, and obtain the supernatant in the culture bottle after the cultivation is completed.

[0027] (3) The supernatant collected for 72 hours was poured into ultrafiltration centrifuge tubes for centrifugation, and the umbilical cord mesenchymal stem cell-derived conditional culture medium was obtained after filtration through a filter membrane, and stored in a -80°C refrigerator for future use.

[0028] scratch model

[0029] This example uses human im...

Embodiment 2

[0040] A preparation method of skin repair liquid, specifically comprising the following steps:

[0041] (1) Human umbilical cord mesenchymal stem cells are cultivated in a culture bottle in a carbon dioxide incubator to make the density reach 90-95%.

[0042] (2) After washing the cells with α-MEM medium, add new α-MEM medium, continue to culture in the carbon dioxide incubator, and obtain five supernatants after culturing for 12h, 24h, 48h, 72h, and 96h respectively.

[0043] (3) Pour the collected supernatants into ultrafiltration centrifuge tubes for centrifugation, and obtain umbilical cord mesenchymal stem cell-derived conditional culture medium after filtration through a filter membrane, and store them in a refrigerator for future use.

[0044] The conditioned culture medium derived from umbilical cord mesenchymal stem cells of different culture periods was collected, and the scratch model experiment was carried out by the same method as in Example 1, and the results we...

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Abstract

The invention discloses a skin repairing liquid and a preparation method thereof and belongs to the technical field of skin repairing. The skin repairing liquid is a conditional culture liquid derivedfrom umbilical cord mesenchymal stem cells. Compared with a method that mesenchymal stem cells are directly applied to treatment on skin wounds, the skin repairing liquid disclosed by the invention avoids security problems related to living cell transplanting and cell proliferation, such as immune compatibility, tumorigenicity and propagation of infectious diseases; in addition, security, dosagesand effects of components in a conditional culture medium can be evaluated by using a method similar to a method for evaluating conventional medicines; the skin repairing liquid disclosed by the invention is easy to produce and prepare, can be subjected to freeze drying preservation, is easy to package and transport, does not take the time for anabiosis, stem cell proliferation and the like before treatment, and is convenient to use.

Description

technical field [0001] The invention relates to a skin repair liquid and a preparation method thereof, belonging to the technical field of skin wound repair. Background technique [0002] Skin is the main protective barrier of the body, and skin with complete form and function can effectively reduce or even avoid damage to the body caused by harmful physical and chemical factors. Skin trauma is a growing problem and has become a great health and economic burden. Skin trauma due to factors such as burns, trauma, surgery, radiation damage, diabetes, etc. is a major challenge for modern medicine. If the skin is wounded, it is prone to infection, and if it forms a chronic non-healing wound, it will seriously affect the quality of life of the patient. Therefore, skin wounds must be treated quickly and effectively. There are many methods and means for treating skin wounds at present, but the curative effect obtained is not satisfactory. [0003] The cell proliferation and migra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/51A61P17/00A61P17/02C12N5/0775
CPCA61K9/0014A61K35/51A61P17/00A61P17/02C12N5/0665
Inventor 蒲磊李亚雄杨应南刘博宇张静孟平张雅永韩燊
Owner YANAN HOSPITAL OF KUNMING CITY
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