Manufacturing method of plant tissue semi-thin section

Abstract

The invention discloses a manufacturing method of a plant tissue semi-thin section. The manufacturing method of the plant tissue semi-thin section comprises the following steps: selecting plant tissue, and sequentially carrying out fixation, dehydration, pre-permeation, permeation, embedding, section cutting, section baking, resin removal and dyeing; adopting a steel blade for sectioning; carryingout dyeing by sequentially adopting crocus sativus and solid green. The steel blade commonly used in the paraffin section is used as a section cutter, so that the application range of the resin section is expanded. According to the dyeing method, a crocus sativus-solid green re-dyeing method is used; compared with a method adopting toluidine blue dye liquor, the method has the advantages that theprice of the crocus sativus is lower than that of toluidine blue, the storage requirement is not high, the crocus sativus can be stored at room temperature, the preparation process is simple, convenient and easy to implement, precipitates are not prone to being generated, different tissue structures show different colors, different structures and components of tissues can be more obviously reflected, and the quality and the image effect of sections are improved.

Description

technical field [0001] The invention relates to a method for making semi-thin slices of plant tissue, which belongs to the field of biotechnology. Background technique [0002] Resin semi-thin sectioning is a widely used technique for morphological research, and it is often used for ultrathin section tissue positioning in TEM sample preparation. Compared with paraffin sections, the production procedure and steps of resin semi-thin sections are simplified, the cycle is shorter, and the thickness of the sections is thinner, generally only 2-7 μm in thickness. Therefore, the clarity and resolution of semi-thin section images It is better than paraffin section, and the field of view is larger than that of ultrathin section, which is conducive to obtaining high-quality light microscope images. There are multiple steps involved from sampling to dyeing, and any problems in any link will affect the quality of sample preparation and even lead to sample preparation failure. A good d...

AI Technical Summary

Problems solved by technology

Toluidine blue is a commonly used synthetic dye. The dyes commonly used in the preparation of plant semi-thin slices belong to the quinone imine dyes. The cations in them have a dyeing effect, and the acidic substances of tissue cells combine with the cations to be dyed. , so the staining is a single staining, lack of layers, no contrast, and the toluidine blue stain solution is easy to precipitate and denature, all of which will affect the quality and image effect of semi-thin sections

Therefore, in view of the disadvantages of toluidine blue staining and expensive glass knife in the prior art, it is necessary to provide a new method for making semi-thin slices of plant tissue

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