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Aesculin utilization test medium, preparation method of medium, kit and sample analyzer

A escin and culture medium technology, which is applied in the field of microbial detection, can solve the problems of inaccurate interpretation of experimental results and complicated pretreatment, and achieve the effects of obvious color changes, simple operation, and improved accuracy

Pending Publication Date: 2020-05-08
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, during the long-term research and development process, the inventors of the present application found that the pretreatment of most of the current determination methods is complicated, and the interpretation of the experimental results is not accurate

Method used

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  • Aesculin utilization test medium, preparation method of medium, kit and sample analyzer
  • Aesculin utilization test medium, preparation method of medium, kit and sample analyzer
  • Aesculin utilization test medium, preparation method of medium, kit and sample analyzer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The preparation method of escin utilization test medium 1 comprises:

[0058] Step (1): In parts by weight, weigh 5 parts of tryptone, 1 part of yeast extract, 3 parts of sodium chloride, 0.5 part of aescin, 0.5 part of ferrous sulfate, and 1000 parts of distilled water.

[0059] Step (2): The tryptone, yeast extract, sodium chloride, aescin, ferrous sulfate, and distilled water are respectively subjected to membrane filtration treatment to retain microorganisms.

[0060] Step (3): Mix the filtered tryptone, yeast extract, sodium chloride, and aescin, add distilled water, stir until completely dissolved, and obtain a mixed solution.

[0061] Step (4): Sterilize the mixed solution obtained in step (3), wherein the sterilization temperature is 100° C., and the sterilization time is 45 minutes.

[0062] Step (5): cooling the sterilized mixed solution, adding ferrous sulfate to the cooled mixed solution, and completely dissolving it to obtain a escin utilization test mediu...

Embodiment 2

[0064] The preparation method of escin utilization test medium 2 comprises:

[0065] Step (1): In parts by weight, weigh 7 parts of tryptone, 3 parts of yeast extract, 4 parts of sodium chloride, 0.8 part of aescin, 0.8 part of ferrous sulfate, and 1000 parts of distilled water.

[0066] Step (2): The tryptone, yeast extract, sodium chloride, aescin, ferrous sulfate, and distilled water are respectively subjected to membrane filtration treatment to retain microorganisms.

[0067] Step (3): Mix the filtered tryptone, yeast extract, sodium chloride, and aescin, add distilled water, stir until completely dissolved, and obtain a mixed solution.

[0068] Step (4): Sterilize the mixed solution obtained in step (3), wherein the sterilization temperature is 110° C., and the sterilization time is 40 minutes.

[0069] Step (5): cooling the sterilized mixed solution, adding ferrous sulfate to the cooled mixed solution, and completely dissolving it to obtain a escin utilization test medi...

Embodiment 3

[0071] The preparation method of escin utilization test medium 3 comprises:

[0072] Step (1): In parts by weight, weigh 10 parts of tryptone, 5 parts of yeast extract, 5 parts of sodium chloride, 1 part of aescin, 1 part of ferrous sulfate, and 1000 parts of distilled water.

[0073] Step (2): The tryptone, yeast extract, sodium chloride, aescin, ferrous sulfate, and distilled water are respectively subjected to membrane filtration treatment to retain microorganisms.

[0074] Step (3): Mix the filtered tryptone, yeast extract, sodium chloride, and aescin, add distilled water, stir until completely dissolved, and obtain a mixed solution.

[0075] Step (4): Sterilize the mixed solution obtained in step (3), wherein the sterilization temperature is 120° C., and the sterilization time is 35 minutes.

[0076] Step (5): cooling the sterilized mixed solution, adding ferrous sulfate to the cooled mixed solution, and completely dissolving it to obtain a escin utilization test medium 3,...

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Abstract

The invention discloses an aesculin utilization test medium, a preparation method of the medium, a kit and a sample analyzer. The aesculin utilization test medium at least comprises aesculin, ferroussulfate, a carbon source, a nitrogen source and inorganic salt. By the above mode, misinterpretation on a test result can be reduced, and the detection accuracy can be improved.

Description

technical field [0001] The present application relates to the technical field of microbial detection, in particular to a escin utilization test medium, a production method, a kit, and a sample analyzer. Background technique [0002] With the development of the economy and the continuous improvement of people's living standards, the awareness of human health is also constantly improving. At present, methods for detecting microorganisms include machine-based methods such as immunoassay, ELISA, gas chromatography mass spectrometry, and liquid chromatography mass spectrometry. [0003] However, during the long-term research and development process, the inventors of the present application found that the pretreatment of most of the current determination methods is complicated, and the interpretation of the experimental results is not accurate. Contents of the invention [0004] The technical problem mainly solved by this application is to provide a escin utilization test mediu...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12M1/34
CPCC12Q1/045
Inventor 何圣侠胡彦勇
Owner SHENZHEN DYMIND BIOTECH
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