Hybridoma cell strain 1D4 secreting anti-CLas transport protein Ctp2 monoclonal antibody, monoclonal antibody and application

Abstract

The invention discloses a hybridoma cell strain 1D4 secreting an anti-CLas transport protein Ctp2 monoclonal antibody, the monoclonal antibody and application. The hybridoma cell strain 1D4 was preserved in China Center for Type Culture Collection (CCTCC) on November 26, 2019, the preservation number is CCTCC NO: C2019295, and the anti-CLas transport protein Ctp2 monoclonal antibody mAb 1D4 is secreted by the hybridoma cell strain 1D4. The anti-CLas transport protein Ctp2 monoclonal antibody mAb 1D4 secreted by the hybridoma cell strain 1D4 can recognize Candidatus Liberibacter spp. transportprotein Ctp2, and it is proved through an immunoblotting reaction that the monoclonal antibody mAb 1D4 can specifically recognize the transport protein Ctp2 of pathogenic bacteria CLas. The monoclonalantibody mAb 1D4 can be used for preparing preparations and drugs for inhibiting or controlling citrus Huanglongbing, and therefore, the citrus Huanglongbing is prevented and treated, and good popularization and application prospects are achieved.

Description

technical field [0001] The invention relates to the technical field of cellular immunity, in particular to a hybridoma cell line 1D4 secreting an anti-CLas transporter Ctp2 monoclonal antibody, the monoclonal antibody and its application. Background technique [0002] Citrus huanglongbing is a devastating disease of citrus, causing huge economic losses worldwide. After the citrus plants are infected with Huanglongbing, the leaves shrink and fall, the seed setting rate decreases, the fruit is small, and the color is yellow and green, which eventually leads to the death of the plants. Moreover, the citrus Huanglongbing disease is highly contagious and can spread to the entire orchard in a short period of time. The pathogenic bacteria of citrus huanglongbing is Gram-negative bacteria, which has three pathogenic varieties, namely, the Asian species C andidatus Liberibacter asiaticus (CLas), the African species Candidatus Liberibacter africa nus (CLaf), and the American species C...

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Problems solved by technology

However, there are many kinds of efflux proteins of citrus chrysanthemum. It has not been reported which efflux protein is used as an antigen to produce monoclonal antibodies to detect or block citrus chrysanthemum best, and there is no research against citrus chrysanthemum in Asia yet. Research Report on Monoclonal Antibody of Species (CLas) Transporter Ctp2 (WP_01582 4909.1)

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