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Preparation method of receptaculum nelumbinis total flavonoids

A technology of total flavonoids and lotus pods, applied in the field of preparation of lotus pods total flavonoids, can solve the problems of single inspection index, failure to fully reflect the true content of flavonoids, and inability to fully represent flavonoids

Inactive Publication Date: 2020-05-08
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inspection index of this method is single and cannot fully represent the content of flavonoids, and acid hydrolysis is affected by many factors, which cannot fully reflect the true content of flavonoids in lotus pods, nor can it reflect the main flavonoids in lotus pods Myricetin-3-O-β-D-glucuronide, quercetin-3-O-β-D-glucuronide, hyperoside, isoquercitrin, kaempferol-3-O- β-D-galactoside, kaempferol-3-O-β-D-glucuronide, isorhamnetin-3-O-β-D-galactoside, clovetin-3-O-β- The content of D-glucoside has certain limitations

Method used

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  • Preparation method of receptaculum nelumbinis total flavonoids

Examples

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Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of total flavonoids of lotus pod: 2 kg of lotus pod medicinal material, reflux extracting 16 times the volume of the medicinal material in 50% ethanol aqueous solution for 3 times, filtering the extract, concentrating under reduced pressure to no ethanol, adding 10 times the volume of water to dissolve , filtered, HPD-722 macroporous resin on the filtrate, eluted with 5 BV of water successively, and then eluted with 50% ethanol aqueous solution of 5 BV, collected 50% ethanol aqueous solution eluent; Concentrated under reduced pressure to dryness, obtained crude extract After adding 40 times the volume of the crude extract to dissolve in water, go to polyamide column chromatography, elute with 5BV of water, then elute with 5BV of 30% ethanol in water, collect the eluate of 30% ethanol in water, and decompress Concentrate to dryness to obtain an extract; add 40 times the volume of the extract to dissolve in water to obtain an aqueous solution of the ex...

Embodiment 2

[0024] A preparation method of total flavonoids of lotus pod: 2 kg of lotus pod medicinal material, 14 times the volume of medicinal material, reflux extraction of 70% ethanol aqueous solution for 3 times, filtering the extract, combining the filtrate, concentrating under reduced pressure to no ethanol, and then adding Dissolve 10 times the volume of water, filter, apply HPD-400 macroporous resin to the filtrate, and elute with 5 BV of water in turn, then elute with 5 BV of 50% ethanol aqueous solution, collect the eluate of 50% ethanol aqueous solution, and concentrate under reduced pressure to dryness to obtain a crude extract; add 40 times the volume of the crude extract to dissolve in water, apply polyamide column chromatography, elute with 5BV of water, then elute with 5BV of 50% ethanol aqueous solution, and collect 50% ethanol aqueous solution. Deliquified, concentrated to dryness under reduced pressure to obtain the extract; add 40 times the volume of the extract to dis...

Embodiment 3

[0027] A preparation method of total flavonoids of lotus pod: 2 kg of lotus pod medicinal material, the volume concentration of 20 times the volume of the medicinal material is 95% ethanol aqueous solution and reflux extraction for 3 times, the extract is filtered, the filtrate is combined, and concentrated under reduced pressure to no ethanol, Add 10 times the volume of water to dissolve, filter, apply D-101 macroporous resin to the filtrate, and elute with 5 BV of water in turn, then elute with 5 BV of 50% ethanol aqueous solution, collect the eluate of 50% ethanol aqueous solution, and depressurize Concentrate to dryness to obtain a crude extract; add 40 times the volume of the crude extract to dissolve in water, apply polyamide column chromatography, elute with 5BV of water, and then elute with 5BV of 30% ethanol aqueous solution, collect 30% ethanol aqueous solution The eluate was concentrated to dryness under reduced pressure to obtain an extract; adding 40 times the volu...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine, and concretely discloses a preparation method of receptaculum nelumbinis total flavonoids. According to the method, receptaculum nelumbinis is used as a raw material, is subjected to reflux extraction by an ethanol aqueous solution and is concentrated; a concentrate is dissolved in water and is then subjected to macroporous adsorption resin chromatography purification to obtain a crude extract; the crude extract is dissolved in water and is then subjected to polyamide column chromatography purification to obtain an extract; a water solution of the extract is firstly extracted through ethyl acetate, and is then extracted through n-butanol; and an n-butanol extraction solution is subjected to reduced pressure concentration to a dry state to obtain an extract of the receptaculum nelumbinis total flavonoids. The extract of the receptaculum nelumbinis total flavonoids prepared by the method contains 8 kinds of flavonoid compounds, and the sum of the contents of the flavonoid compounds exceeds 60 percent.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a preparation method of total flavonoids of lotus pod. Background technique [0002] The lotus pod is a plant lotus of the water lily family ( Nelumbo nucifera Gaertn.)’s dry receptacle has the effects of removing blood stasis and stopping bleeding, and is used for metrorrhagia, hematuria, bleeding from hemorrhoids, postpartum stasis, and endless lochia. Chemical and pharmacological studies have shown that lotus pod mainly contains flavonoids and proanthocyanidins, which have anti-oxidation, anti-tumor, anti-aging, liver protection, memory improvement, anti-radiation and blood fat-lowering effects. The lotus pod is a by-product in the processing of lotus seeds, which is discarded as waste and not utilized. The lotus pod contains a large amount of nutrients and rich flavonoids. Exploring its potential medicinal use is also meaningful for resour...

Claims

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Application Information

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IPC IPC(8): A61K36/62
CPCA61K36/62A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 吴岩斌吴锦忠吴建国陈舒婉
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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