Recombinant hansenula polymorpha cell disruption buffer solution for expressing hand-foot-and-mouth disease vaccine antigen as well as preparation method and application thereof

A hand-foot-and-mouth disease vaccine and cell fragmentation technology, which is applied in the field of recombinant Hansenula yeast cell fragmentation buffer and its preparation, can solve the problems of unsatisfactory cell fragmentation rate, target protein content and target antigen content, etc., so as to improve the fragmentation rate. Effect

Pending Publication Date: 2020-05-05
SHENZHEN KANGTAI BIOLOGICAL PROD
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] However, the inventor found in the process of implementing the present invention that under the same process conditions, high-pressure homogenization was used for crushing, and the tromethamine (Tris) buffer commonly used in the market was used for cell crushing, and the cell crushing product of recombinant Hansenula spp. Cell disruption rate, target protein content and target antigen content are not ideal

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  • Recombinant hansenula polymorpha cell disruption buffer solution for expressing hand-foot-and-mouth disease vaccine antigen as well as preparation method and application thereof
  • Recombinant hansenula polymorpha cell disruption buffer solution for expressing hand-foot-and-mouth disease vaccine antigen as well as preparation method and application thereof
  • Recombinant hansenula polymorpha cell disruption buffer solution for expressing hand-foot-and-mouth disease vaccine antigen as well as preparation method and application thereof

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Embodiment Construction

[0025] Describe in detail below the embodiment of the recombinant Hansenula cell disruption buffer that expresses the HFMD vaccine antigen provided by the present invention with reference to accompanying drawing;

[0026] Table 1. Cell Disruption Buffer Formula

[0027] Element formula Element formula Water for Injection About 1000.0g / L trometamol 1.00-100.00g / L glycerin 1.00-100.00g / L Medicinal Sodium Chloride 10.00-100.00g / L

[0028] In some specific embodiments, hydrochloric acid 1-10ml / L is also included.

[0029] In some specific embodiments, the tromethamine is 10.00-80.00 g / L.

[0030] In some specific embodiments, the medicinal sodium chloride is 10.00-80.00 g / L.

[0031] In some specific embodiments, the glycerol is 10.00-50.00 g / L.

[0032] In some specific embodiments, the concentration of the medicinal sodium chloride is 0.1-0.5ml / L.

[0033] In some specific embodiments, the solvent is water for injection.

[0034] Tr...

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Abstract

The embodiment of the invention discloses a recombinant hansenula polymorpha cell disruption buffer solution for expressing a hand-foot-and-mouth disease vaccine antigen as well as a preparation method and application of the recombinant hansenula polymorpha cell disruption buffer solution. The cell disruption buffer solution is prepared from the following components: 1.00 g/L to 100.00 g/L of tromethamine, 10.00 g/L to 100.00 g/L of medicinal sodium chloride and 1.00 g/L to 100.00 g/L of glycerinum. By implementing the method disclosed by the invention, the breakage rate, the target protein content and the target antigen content of the recombinant hansenula polymorpha cell breakage product can be obviously improved.

Description

technical field [0001] The invention relates to the technical field of preparation of hand, foot and mouth disease vaccine expressed by recombinant Hansenula, in particular to a recombinant Hansenula cell breaking buffer expressing hand, foot and mouth disease vaccine antigen and its preparation method and application. Background technique [0002] During the preparation of the HFMD vaccine expressed by the recombinant Hansenula yeast, solid-liquid separation of the fermentation product and cell disruption are required, and the final goal is to collect the target protein and target antigen. The commonly used cell disruption method in the prior art is high-pressure homogenization, and a large amount of cell disruption buffer must be used in the process. The quality of the cell disruption buffer directly affects the cells in the preparation process of the hand, foot and mouth disease vaccine expressed by recombinant Hansenula. The crushing effect, target protein content, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06A61K39/125A61P31/14C12R1/78
CPCC12N1/063A61K39/12A61P31/14C12N2770/32334
Inventor 李勇刚钟礼军彭逸云李进曾银怀刘洁刘海涛
Owner SHENZHEN KANGTAI BIOLOGICAL PROD
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