Anti-emulsification precipitant for assisting extraction of a weak-polarity-non-polar target and application thereof

Abstract

The invention discloses an anti-emulsification precipitant for assisting extraction of a weak-polarity-non-polar target and application thereof. The anti-emulsification precipitant is characterized bymainly comprising the following raw materials in proportion: 500mL of 1mol / L ammonium acetate buffer solution, 2g of polyethylene glycol, 200mL of lower alcohol, 500mL of acetonitrile and 20mL of n-octanol, wherein the pH is adjusted to 4.8 by formic acid. A use method comprises the following steps of adding ethanol with the same volume as a to-be-tested sample, the anti-emulsification precipitant with the volume being 9 times that of the to-be-tested sample and a non-polar solvent with the volume being 5 times that of the to-be-tested sample, performing high-speed homogenization, performingcentrifugation 10 to obtain two layers of clear liquid, collecting supernatant liquid, then repeatedly extracting the supernatant liquid for 1-2 times by the non-polar solvent with the volume being 5times that of the to-be-tested sample, and combining all the supernatant liquid to obtain all weak-polarity-non-polar target components. The anti-emulsification precipitant has the advantages that theemulsification phenomenon during extraction of the non-polar solvent can be overcome, and the anti-emulsification precipitant has a strong precipitation effect and a purification effect of impurity removal and also has a demulsification effect on matrixes such as dairy products.

Description

technical field [0001] The invention relates to a precipitation purifying agent for food and biological substrates, in particular to an anti-emulsification precipitation agent for assisting the extraction of weakly polar-nonpolar target objects and its application. Background technique [0002] Non-polar solvents such as n-hexane and petroleum ether are commonly used to extract fat-soluble targets in the non-polar-weak polar range. Not only does the extraction have high extraction efficiency, but it is also incompatible with water and separates into layers, which is very convenient for separation. However, for complex matrix foods such as infant formula milk powder, feed, starchy foods, animal offal, etc., when the system contains water, it is easy to form an emulsification phenomenon when the system contains water and vortex extraction with a non-polar solvent, and the organic phase and The separation of the aqueous phase is not obvious, or even completely mixed together, a...

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Problems solved by technology

However, for complex matrix foods such as infant formula milk powder, feed, starchy foods, animal offal, etc., when the system contains water, it is easy to form an emulsification phenomenon when the system contains water and vortex extraction with a non-polar solvent, and the organic phase and The separation of the aqueous phase is not obvious, or even completely mixed together, and the separation is very difficult. Not only is it time-consuming, but the recovery and repeatability of the extraction are of low quality.

Especially in milk powder samples, non-polar solvents cannot even break the emulsion, and non-polar components such as fats and oils are completely encapsulated, making it extremely difficult to dissociate and cannot be extracted

Commonly used protein precipitation reagents such as trichloroacetic acid and acetic acid solution (5-10%) have poor precipitation effects; although perchloric acid solution (5%) has an excellent precipitation effect, it is not suitable for targets that are sensitive to acidic or strong oxidation Very destructive, poor recovery rate

Potassium ferrocyanide and zinc acetate are used as protein precipitation clarifiers. The reaction precipitation effect of zinc acetate and potassium ferrocyanide is obvious, and the ability to remove protein is very strong. However, it cannot break the emulsion, and some target objects are easily wrapped in the precipitate. low recovery rate

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