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Online detection method for glucoside content in stevioside extracting solution

A detection method and extraction liquid technology, which is applied in the direction of measuring devices, material analysis through optical means, instruments, etc., can solve the problems of time-consuming, etc., and achieve the effect of convenient detection and improved detection efficiency

Inactive Publication Date: 2020-04-28
山东金璋隆祥智能科技有限责任公司
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Problems solved by technology

[0003] At present, the detection methods of steviol glycosides mainly include thin-layer chromatography, spectrophotometry, capillary electrophoresis, high-performance liquid chromatography, etc. These detection methods have the disadvantages of requiring pretreatment and taking a long time. Therefore, a rapid The method for determining stevioside is an urgent problem to be solved

Method used

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  • Online detection method for glucoside content in stevioside extracting solution
  • Online detection method for glucoside content in stevioside extracting solution
  • Online detection method for glucoside content in stevioside extracting solution

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Embodiment

[0050] Such as Figure 1-7 As shown, the embodiment of the present invention provides an online detection method of glycoside content in stevioside extract, comprising the following steps:

[0051] S1. Establish a detrending correction preprocessing method, use the least squares method to fit the absorbance and wavelength of the original spectrum into a trend line, and then subtract the trend line from the original spectrum to eliminate the baseline drift in the original spectrum, including the following formula:

[0052]

[0053]

[0054] in is the spectrum after detrending correction, Y j is the original spectrum, is the trend line obtained by binomial linear fitting;

[0055] S2. Establish a multiple linear regression modeling method. The basic tasks of multiple linear regression analysis include:

[0056] 1) Establish the multiple linear regression equation of the dependent variable on multiple independent variables based on the actual observation values ​​of th...

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Abstract

The invention provides an online detection method for the glucoside content in a stevioside extracting solution, and relates to the technical field of glucoside content detection. The online detectionmethod for the glucoside content in a stevioside extracting solution comprises the following steps: S1, establishing a detrending correction pretreatment method; S2, establishing a multiple linear regression modeling method; S3, installing a fluid sample measuring device and a sampling valve of a GSA103 type near-infrared spectrometer at an outlet of a liquid flowing-out pipeline of a macro-porous adsorption tank, immersing the fluid sample measuring device of the instrument in liquid in the pipeline, and fixing the instrument; and S4, setting parameters of the spectrograph, setting the number of scanning times to be 30, setting the number of sampling times to be 20, setting the wavelength scanning range to be 1550-1950 nm, and setting the wavelength increment to be 2 nm. According to theinvention, real-time online detection can be realized, the purpose of continuous production is achieved, information of all samples can be detected, the detection is convenient, and the detection efficiency of the glucoside content is greatly improved; and the detection mode does not need any treatment on the sample, the sample is not damaged, and the detection process is green and environment-friendly.

Description

technical field [0001] The invention relates to the technical field of glycoside content detection, in particular to an online detection method for glycoside content in a stevioside extract. Background technique [0002] Stevioside is a kind of glycoside substance, which is derived from the extract of stevia rebaudiana leaves. Its purity and the ratio of its main components stevioside and rebaudioside A (RA) in stevioside directly affect the quality of stevioside. [0003] At present, the detection methods of steviol glycosides mainly include thin-layer chromatography, spectrophotometry, capillary electrophoresis, high-performance liquid chromatography, etc. These detection methods have the disadvantages of requiring pretreatment and taking a long time. Therefore, a rapid The method for determining stevioside is an urgent problem to be solved. Contents of the invention [0004] (1) Solved technical problems [0005] Aiming at the deficiencies of the prior art, the presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/3577
CPCG01N21/359G01N21/3577
Inventor 邹振民董海平孙茂耿龙飞朱传港
Owner 山东金璋隆祥智能科技有限责任公司
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