Method for detecting endogenous hormones in pinus massoniana branches
An endogenous hormone, masson pine technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as cumbersome detection procedures, and achieve the effects of solving cumbersome detection procedures, simple procedure steps, and saving space.
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[0038] A method for detecting endogenous hormones in pine branches, comprising the following steps:
[0039] A, get 3g masson pine branches and drop into liquid nitrogen and grind into powder, then add the massfraction of 45g and be 80% methyl alcohol, obtain mixed solution;
[0040] B. Add 0.48g of vitamin C to the mixed solution in step A and place it at 3-5°C for 2 hours, then centrifuge to obtain 3.2g of precipitate;
[0041] C. Take the precipitate in step B and add 32g of methanol, then centrifuge and take the supernatant, and pass the supernatant through polyvinylpolypyrrolidone PVPP column and diethylaminoethyl cross-linked dextran gel successively After the column is filtered, take the filtrate;
[0042] D. Dry the filtrate in step C, add analytical pure methanol to dilute to 1mL and use a disposable syringe to collect the dilute solution, then filter it with a 0.22μm microporous membrane and use high performance liquid chromatography-mass spectrometry The content o...
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