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Method for high-throughput rapid evaluation and/or screening of saline-alkali tolerant plants

A plant and salt-tolerant technology, applied in the direction of measuring devices, fluorescence/phosphorescence, color/spectral characteristics measurement, etc., can solve the problems of limited space and site area, long salt-tolerant occupation, long growth cycle, etc., and achieve simple equipment Easy to obtain, high energy and short experimental period

Inactive Publication Date: 2020-04-17
CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Woody plants tend to be larger in size and have a longer growth cycle, so the evaluation of the salt tolerance of woody plants (including the cultivation of seedlings to the determination of indoor controlled environmental conditions or field natural environmental conditions) takes a long time and large space; on the other hand, the number of species and varieties screened at the same time is also limited by space and site area

Method used

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  • Method for high-throughput rapid evaluation and/or screening of saline-alkali tolerant plants
  • Method for high-throughput rapid evaluation and/or screening of saline-alkali tolerant plants
  • Method for high-throughput rapid evaluation and/or screening of saline-alkali tolerant plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Elaeagnus sativa with high salt tolerance was used as the experimental material, among which, Ale sativa (ALE) and Yinchuan (YC) were two provenances, and the salt tolerance of ALE provenance was slightly stronger than that of YC provenance.

[0028] NaCl solution configuration: 150mM NaCl solution is used for salt treatment, and a sufficient amount of salt solution needs to be prepared in advance according to the types of test tree species and the test repetition settings for future use.

[0029] Use branch scissors to cut out branches of the same part of Elaeagnus erae to be measured at the same age or growth time from the field, with a length of about 15-30 cm, soak in clean water until the experimental treatment (to prevent water loss), and use as much as possible , to ensure that the branches and leaves do not wilt while soaking in the salt solution.

[0030] The prepared plant branch to be tested is put into the 50ml centrifuge tube that fills 150mM NaCl solution ...

Embodiment 2

[0038] The test material is the bamboo willow whose salt tolerance is lower than that of Elaeagnus sativa, and the others are the same as in Example 1.

[0039] See Table 1 for the results of the total chlorophyll content and Fv / Fm of the bamboo willow blank control group and the salt treatment group at each treatment time point.

[0040] The △Fv / Fm of bamboo and willow blank control group and salt treatment group after treatment for 72 hours can be seen figure 2 .

[0041] Table 1

[0042]

[0043] The results of observing and measuring the phenotype after different treatments of 0, 24, 48 and 72 of the test materials and the changes of the light energy conversion efficiency Fv / Fm of the total chlorophyll content and the maximum PSII show that: 1) different tree species, different provenances of the same tree species The symptoms caused by the response to salt treatment were different: after 24 hours of salt treatment, a small amount of leaves on the branches of bamboo ...

Embodiment 3

[0045] The detached leaves of the salt-sensitive Naso Nijo barley variety and the salt-tolerant barley variety TX 9425 were treated with water (blank control) and 50mmol / L NaCl solution, respectively, and the two barley varieties were measured after 0h and 48h of the blank control and salt treatment. Varieties of chlorophyll SPAD (relative chlorophyll content) value and CCI (chlorophyll content index) value. The chlorophyll SPAD value is measured by a chlorophyll measuring instrument SPAD-502, calculated according to the amount of transmitted light from the leaves, and the SPAD value is proportional to the absolute content of chlorophyll in the leaves. The chlorophyll content index CCI is measured by a CCM-200 portable chlorophyll meter and calculated by measuring the different absorbance of leaves at two wavelengths of red light and near-infrared light.

[0046] See image 3 ; The results of the chlorophyll SPAD value in the barley leaves in vitro after the blank control (cl...

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Abstract

The invention relates to a method for high-throughput rapid evaluation and / or screening of saline-alkaline tolerant plants. The method comprises steps of 1) obtaining in vitro branches of a tested plant to obtain a tested material; 2) treating the tested material in salt and / or alkali solution to obtain a treated material, and placing the tested material in the clear water to be treated to obtaina blank control material; 3) determining the chlorophyll content and / or chlorophyll fluorescence parameters of the treatment material and the blank control material, and 4) comparing the chlorophyll content and / or chlorophyll fluorescence parameters of the treatment material with the chlorophyll content and / or chlorophyll fluorescence parameters of the blank control material.

Description

technical field [0001] The invention relates to a method for rapidly evaluating and / or screening saline-alkali-tolerant plants with high throughput. Background technique [0002] Salt damage is one of the most serious environmental problems in the world. 20% of the world's arable land and nearly half of the irrigated land are threatened by different degrees of salt damage. my country alone has a saline-alkali land area of ​​about 100 million hm 2 , accounting for about 1 / 10 of the country's land area, and the area of ​​secondary salinized land is still increasing, which has become an important factor restricting the development of agricultural and forestry production in many areas of our country. The current improvement measures for saline-alkali land mainly include physical measures, chemical measures and biological measures, among which biological measures have been recognized and accepted by more and more countries and regions because of their low investment, no pollutio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/3563G01N21/359G01N21/31
CPCG01N21/314G01N21/3563G01N21/359G01N21/6486
Inventor 杨秀艳谢尔盖·沙巴拉张会龙姜磊褚建民甘红豪武海雯朱建峰
Owner CHINESE ACAD OF FORESTRY
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