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Preparation method of urea sensor with Janus structure artificial cell mode

A urea sensor and artificial cell technology, applied in the field of catalytic detection, can solve the problems of requiring special instruments and complex urea concentration measurement methods, and achieve the effects of convenient portability and transportation, high selectivity, and good data reproducibility

Active Publication Date: 2020-04-17
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing a urea sensor with a Janus structure artificial cell model in order to solve the problem that the existing urea concentration measurement method is relatively complicated or requires a special instrument

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  • Preparation method of urea sensor with Janus structure artificial cell mode
  • Preparation method of urea sensor with Janus structure artificial cell mode
  • Preparation method of urea sensor with Janus structure artificial cell mode

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specific Embodiment approach 1

[0026] Specific embodiment one: what this embodiment records is a kind of preparation method of the urea sensor with Janus structure artificial cell model, described method step is as follows:

[0027] Step 1, preparation of artificial cell model based on urease

[0028] (1) Preparation of aqueous phase solution: dissolve urease in deionized water to prepare 5-20 mg / mL urease aqueous solution;

[0029] (2) Preparation of oil phase solution: mix tributyrin and oleic acid in a volume ratio of 4:1;

[0030] (3) Add the aqueous phase solution of (1) to the oil phase solution of (2), shake it for 20-30s with a vortex oscillator to obtain an oil-in-water microemulsion, and let it stand for 30 minutes to prepare an artificial cell based on urease Model; the size of the model can be adjusted by controlling the oil-water ratio and vortex time;

[0031] Step 2, preparation of phospholipid-based artificial cell model

[0032] (1) Preparation of phospholipid (dimyristoylphosphatidylcho...

specific Embodiment approach 2

[0037] Specific embodiment two: the preparation method of a kind of urea sensor with Janus structure artificial cell model described in specific embodiment one, in step one (3), the volume ratio of described aqueous phase solution and oily phase solution is 10~20 :1.

specific Embodiment approach 3

[0038]Specific embodiment three: the preparation method of a kind of urea sensor with Janus structure artificial cell model described in specific embodiment one, in step two (2), the volume ratio of described aqueous phase solution and glycerol triacetate is 10: 1~2.

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Abstract

The invention discloses a preparation method of a urea sensor with a Janus structure artificial cell model, which belongs to the field of catalytic detection. The method comprises the following steps:adding a urease aqueous solution into a mixed solution of tributyrin and oleic acid, oscillating and performing standing to prepare a urease-based artificial cell model; preparing a phospholipid solution, adding the phospholipid solution into 20-40 [mu] L of glyceryl triacetate, oscillating and performing standing for 30 min to obtain an artificial cell model based on phospholipid; and oscillating the two settled artificial cell models to obtain two uniformly mixed micro-emulsions, and performing standing to remove a supernatant, thereby obtaining the Janus structure artificial cell model with the urea sensing function. According to the invention, the urea concentration is detected by using the urea sensor of the Janus structure artificial cell model; the method is high in selectivity, simple to operate, convenient to carry and transport, free of special instruments, short in detection time (less than 1 minute) and good in data reproducibility, 1000 times of measurement can be realized by one-time preparation (1mL of artificial cell model), and the linear detection range is 0.1 mg / mL-10mg / mL.

Description

technical field [0001] The invention belongs to the field of catalytic detection, and in particular relates to a preparation method of a urea sensor with a Janus structure artificial cell model. Background technique [0002] In the process of catabolism of the body's substances, the deamination of amino acids produces ammonia, which is synthesized into urea through the ornithine cycle in the liver, and the urea is transported to the kidneys through the blood and excreted through the urine. Urea is one of the final products of amino acid catabolism in the body, so measuring the urea content in the blood can understand the renal function of the body. The determination method of urea can be divided into two categories: one is the direct method, urea reacts directly with a certain reagent to determine its product, the most common is the diacetyl monoxime method, which utilizes diacetyl to condense with urea under strong acid conditions The red 4,5-dimethyl-2-oximidazole compoun...

Claims

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Application Information

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IPC IPC(8): C12Q1/58
CPCC12Q1/58
Inventor 王磊陈海旭刘小曼黄鑫
Owner HARBIN INST OF TECH
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