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Method for producing dephenolized cottonseed protein

A technology for dephenolized gossypol protein and cotton kernel, which is applied in the field of production of dephenolized gossypol protein, can solve the problems of large degree of protein denaturation and high relative energy consumption, and achieves the effects of less destructiveness, good effect, and reduction of thermal denaturation time.

Inactive Publication Date: 2020-04-17
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cottonseed protein contains harmful substances such as gossypol and aflatoxin. In the prior art, the removal of gossypol and aflatoxin is carried out separately. In the patent application number 201010234098.9, it is proposed to remove phenols from cottonseed meal Carry out extrusion and expansion treatment to achieve the purpose of removing free gossypol in cottonseed protein. This method consumes a lot of energy and has a high degree of protein denaturation in order to ensure that the residual amount of gossypol is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Put 200g cotton kernels into a cotton kernel sieve and sieve them into large kernels and fine powder;

[0038] 2. Soften and press the big kernels sieved in step 1, put the processed big kernels into a flat dryer to adjust the moisture, and the percentage of the adjusted moisture is 3%;

[0039] 3. The material prepared by the plate dryer in step 2 is transported to the degreasing extractor, the extraction agent is 6# solvent oil with a solid-liquid ratio of 1:0.5, and the countercurrent extraction is 6 times;

[0040] 4. Transport the material treated in step 3 to the dephenol leaching device, and use basic methanol for countercurrent extraction 6 times, wherein the concentration of basic methanol is 88 °, and the amount of alkali added to the basic methanol is 0.2% of the water in the solution ;

[0041] 5. Spray acidic methanol with the material after step 4 is processed by the dephenol leaching device, and the amount of acid added to the acidic methanol is 1% of...

Embodiment 2

[0046] 1. Put 200g cotton kernels into a cotton kernel sieve and sieve them into large kernels and fine powder;

[0047] 2. Soften and press the big kernels sieved in step 1, put the processed big kernels into a flat dryer to adjust the moisture, and the percentage of the adjusted moisture is 3%;

[0048] 3. The material prepared by the plate dryer in step 2 is transported to the degreasing extractor, the extraction agent is 6# solvent oil with a solid-liquid ratio of 1:1, and the countercurrent extraction is 6 times;

[0049] 4. Transport the material treated in step 3 to the dephenol leaching device, and use basic methanol for countercurrent extraction 6 times, wherein the concentration of basic methanol is 88°, and the amount of alkali added to the basic methanol is 2% of the water in the solution ;

[0050] 5. Spray acidic methanol with the material after step 4 is processed by the dephenol leaching device, and the amount of acid added to the acidic methanol is 1% of the ...

Embodiment 3

[0055] 1. Put 200g cotton kernels into a cotton kernel sieve and sieve them into large kernels and fine powder;

[0056] 2. Soften and press the big kernels sieved in step 1, put the processed big kernels into a flat dryer to adjust the moisture, and the percentage of the adjusted moisture is 3%;

[0057] 3. The material prepared by the plate dryer in step 2 is transported to the degreasing extractor, the extraction agent is 6# solvent oil with a solid-liquid ratio of 1:1, and the countercurrent extraction is 6 times;

[0058] 4. Transport the material treated in step 3 to the dephenol leaching device, and use basic methanol for countercurrent extraction 6 times, wherein the concentration of basic methanol is 88 °, and the amount of alkali added to the basic methanol is 1% of the water in the solution ;

[0059]5. Spray acidic methanol with the material after step 4 is processed by the dephenol leaching device, and the amount of acid added to the acidic methanol is 1% of the ...

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Abstract

The invention relates to a method for producing dephenolized cottonseed protein. The method comprises the following steps: 1, putting cottonseed kernels into a cottonseed kernel sieve, and sieving thecottonseed kernels to obtain large kernels and fine powder; 2, softening and pressing the large kernels screened in the step 1 into blanks, extruding and puffing the fine powder, and carrying out moisture regulation by using a flat-plate drying machine; 3, conveying the material modulated by the flat-plate drying machine in the step 2 to a degreasing extractor, and carrying out countercurrent extraction; 4, conveying the material treated in the step 3 into a dephenolization extractor, and carrying out countercurrent extraction by using alkaline methanol; 5, spraying acidic methanol to the material treated by the dephenolization extractor in the step 4; 6, detecting the pH value of the material treated in the step 5, and conveying the material to a horizontal spiral discharge centrifuge for centrifugal treatment when the material is neutral; and 7, putting a material separated by centrifugal treatment in the step 6 into an evapo-separated machine for low-temperature drying to obtain the dephenolized cottonseed protein. The method provided by the invention has the effects of low energy consumption, less protein denaturation and simultaneous removal of gossypol and aflatoxin.

Description

technical field [0001] The invention relates to a method for producing dephenolized cottonseed protein, which belongs to the technical field of cotton kernel processing. Background technique [0002] Dephenolized cottonseed protein is a high-protein product obtained by removing harmful substances from cotton kernels. As a high-quality protein feed, dephenolized cottonseed protein has a crude protein content ≥ 50%, crude fiber ≤ 8%, and moisture ≤ 6%. , the main component of crude protein is globulin, about 90%, followed by gluten. Three globulins, 2S, 7S, and 12S, can be obtained by ultracentrifugation, of which 2S exists outside the protein body, accounting for about 30%. Dephenolized cottonseed protein has the advantages of high dry matter content, high protein content, and high essential amino acid content. has broad application prospects. [0003] Cottonseed protein contains harmful substances such as gossypol and aflatoxin. In the prior art, the removal of gossypol an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 韩文杰朱丹丹田令善张亚辉
Owner CHENGUANG BIOTECH GRP CO LTD
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