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Diacetoxyscirpenol competitive air pressure immunosensor and detection method

A Fusarenol-competitive Fusarenol-based technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of increasing agricultural product quality and safety risks, complicated sample processing, and inconvenient on-site testing. Strong catalytic ability, strong signal amplification ability, easy to operate

Active Publication Date: 2020-04-14
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of agricultural products involves many links, and mycotoxin pollution is inevitable. my country has set limits on the main mycotoxins in food, but there is no limit on snake toxins
Thereby increasing the hidden dangers of the quality and safety of agricultural products
[0003] The existing detection method of Fusarium diacetate is mainly based on the laboratory confirmatory detection technology of large-scale instruments such as high-performance liquid chromatography, the sample processing is complicated, and it is not convenient for on-site detection.

Method used

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  • Diacetoxyscirpenol competitive air pressure immunosensor and detection method
  • Diacetoxyscirpenol competitive air pressure immunosensor and detection method
  • Diacetoxyscirpenol competitive air pressure immunosensor and detection method

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Embodiment 1

[0056] Example 1 Preparation process of gold core platinum shell particle and gold core platinum shell particle modified goat anti-mouse antibody

[0057] Gold-core platinum-shell particles were synthesized as follows: 10 mL of AuNPs solution was added to 200 μL of 3.86 mM H2PtCL4. After heating the mixture to 80°C for 5 minutes with magnetic stirring, 400 μL, 10 mM ascorbic acid was added dropwise to the mixture and maintained at 80°C for 30 minutes. The obtained gold-core platinum-shell particle aqueous solution is naturally cooled and stored for future use.

[0058] Preparation of gold-core-platinum-shell particle-modified antibody: Add 4ug of anti-mouse secondary antibody (goat anti-mouse antibody) to the gold-core platinum-shell particle and mix well. After incubation for 1-3 hours, add blocking agent containing Tween-20 and inert protein Blocked in the solution for 1-3 hours to obtain a solution of gold core platinum shell particle modified goat anti-mouse antibody. Ce...

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Abstract

The invention relates to a diacetoxyscirpenol competitive air pressure immunosensor and a detection method. The diacetoxyscirpenol competitive air pressure immunosensor comprises an anti-diacetoxyscirpenol monoclonal antibody which is independently stored, an elisa strip, a diacetoxyscirpenol-semi-glutamic acid-whey protein conjugate for coating the elisa strip, a hydrogen peroxide solution, platinum nanoparticles or gold core platinum housing nanoparticles of which the surfaces are modified with goat anti-mouse antibodies, and an air pressure detection sensor for detecting air pressure, wherein the anti-diacetoxyscirpenol monoclonal antibody is produced by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO: C201881. The method has the advantages of high sensitivity, portability, low cost, easiness in operation and the like.

Description

technical field [0001] The invention relates to an air pressure immune sensor of a diacetate diacetate competition type air pressure immunosensor and a method for detecting the diacetate diacetate content using the same. Background technique [0002] Diacetoxyscirpenol (Diacetoxyscirpenol, DAS), also known as snake-like toxin, belongs to a kind of trichothecene mycotoxins, mainly produced by Fusarium pirisporium, Fusarium equiseti and Fusarium spp. Born by the metabolism of spores. It mainly pollutes grain and feed, and its toxicity is high, which can damage human and animal bone marrow and other hematopoietic organs, brain and central nervous cell degeneration, lymph nodes, testis and thymus, etc. Therefore, snake toxin pollution is a major risk and hidden danger that threatens the quality and safety of agricultural products and the development of agricultural industries. The production of agricultural products involves many links, and mycotoxin pollution is inevitable. m...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/577
CPCG01N33/54346G01N33/577G01N2333/37
Inventor 张兆威唐晓倩王督张奇李培武
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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