Preparation method and application of electrochemical adapter sensor for quick detecting thrombin
An aptamer sensor and thrombin technology, which is applied in the direction of material electrochemical variables, can solve the problems of less thrombin and accurate detection, and achieve the effects of strong catalytic ability, improved sensitivity, and enhanced response signal
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Embodiment 1
[0025] Example 1 Preparation method of an electrochemical aptasensor for rapid detection of thrombin
[0026] (1) Select thiol-modified thrombin capture probe Apt1 and biotin-modified thrombin reporter probe Apt2; (2) Synthesize gold nanoparticles-modified formyl imidazole-modified reduced graphene AuGS-FI; weigh the mass ratio as 1:1 formyl imidazole modified reduced graphene GS-FI and chloroauric acid, dispersed in water for 24 hours, centrifuged to remove the supernatant, re-dispersed in water, and adjusted the pH-1 NaBH 4 The solution is completely reduced to chloroauric acid, finally, centrifuged and washed three times with ultrapure water, and dried in a vacuum oven;
[0027] (3) Preparation of cobalt palladium nanoparticles CoPdNPs modified thrombin reporter probe CoPdNPs-Apt2
[0028] Add 3 mg of cobalt palladium nanoparticles CoPdNPs with a particle size of about 7 nm to 1.5 mL with a concentration of 0.05 mol L -1 cetyltrimethylammonium bromide CTMAB solution, ul...
Embodiment 2
[0037] Example 2 Preparation method of an electrochemical aptasensor for rapid detection of thrombin
[0038](1) Select thiol-modified thrombin capture probe Apt1 and biotin-modified thrombin reporter probe Apt2; (2) Synthesize gold nanoparticles-modified formyl imidazole-modified reduced graphene AuGS-FI; weigh the mass ratio as 1:1~1.1 formyl imidazole modified reduced graphene GS-FI and chloroauric acid, dispersed in water for 24 hours, centrifuged to remove the supernatant, re-dispersed in water, and adjusted the pH-1 NaBH 4 The solution is completely reduced to chloroauric acid, finally, centrifuged and washed three times with ultrapure water, and dried in a vacuum oven;
[0039] (3) Preparation of cobalt palladium nanoparticles CoPdNPs modified thrombin reporter probe CoPdNPs-Apt2
[0040] Add 4 mg of cobalt palladium nanoparticles CoPdNPs with a particle size of about 8 nm to 2.0 mL with a concentration of 0.05 mol L -1 cetyltrimethylammonium bromide CTMAB solution,...
Embodiment 3
[0049] Example 3 Preparation method of an electrochemical aptasensor for rapid detection of thrombin
[0050] (1) Select thiol-modified thrombin capture probe Apt1 and biotin-modified thrombin reporter probe Apt2; (2) Synthesize gold nanoparticles-modified formyl imidazole-modified reduced graphene AuGS-FI; weigh the mass ratio as 1:1.2 formyl imidazole modified reduced graphene GS-FI and chloroauric acid, dispersed in water for 24 hours, centrifuged to remove the supernatant, re-dispersed in water, and adjusted the pH-1 NaBH 4 The solution is completely reduced to chloroauric acid, finally, centrifuged and washed three times with ultrapure water, and dried in a vacuum oven;
[0051] (3) Preparation of cobalt palladium nanoparticles CoPdNPs modified thrombin reporter probe CoPdNPs-Apt2
[0052] Add 5 mg of cobalt palladium nanoparticles CoPdNPs with a particle size of about 9 nm to 2.5 mL with a concentration of 0.05 mol L -1 cetyltrimethylammonium bromide CTMAB solution, ...
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