Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Endophytic fungus Z1 for increasing biomass of Casuarina equisetifolia in high-salt environment

A technology of endophytic fungi and casuarina, which is applied in the field of microorganisms, can solve the problems of less research on endophytic fungi in woody plants, and achieve the effect of promoting plant biomass and increasing growth

Active Publication Date: 2020-04-14
FUJIAN AGRI & FORESTRY UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A series of previous studies have shown that the symbiont between plant endophytic fungi and the host is beneficial for the host to cope with the adverse environment and promote the growth and development of the plant. Less research on endophytic fungi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Endophytic fungus Z1 for increasing biomass of Casuarina equisetifolia in high-salt environment
  • Endophytic fungus Z1 for increasing biomass of Casuarina equisetifolia in high-salt environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Isolation of casuarina ephedra fungus

[0016] 1. Main equipment

[0017] AX224ZH electronic balance (OHAUS), LS-75HD high-pressure steam sterilizer (Jinan Laibao Medical Equipment Co., Ltd.), JB-CJ-1500FX ultra-clean workbench (Suzhou Jiabao Purification Engineering Equipment Co., Ltd.), DNP-9162 constant temperature culture box (Shanghai Jinghong), ZHWY2111B constant temperature shaking incubator (Shanghai Zhicheng), Beckman ultracentrifuge (USA), etc. Main instruments for strain identification: FQD-48APCR amplification instrument (BIOER), EPS-100 nucleic acid electrophoresis instrument (Shanghai Tianneng Technology), SeqStudio DNA sequencer (USA), mortar, ice pack, foam box, pipette (Thermo), 1.5ml and 2ml EP tubes (Axygen).

[0018] 2. Main Reagents and Media

[0019] Reagents: 70% alcohol, sodium hypochlorite, potato dextrose agar (PDA) medium, potato dextrose (PDB) medium, NaCl, Taq enzyme and PCR related reagents, primers (ITS1 / ITS4), OMEGA fungal ge...

Embodiment 2

[0037] Preparation of bacteria solution: inoculate the activated halotolerant Vitis vinifera endophyte Z1 strain in 60 mL potato dextrose liquid medium, and culture it on a constant temperature shaker for 72 hours (28 °C, 160 r min -1 ). Calculate the number of spores by hemocytometer, and dilute the cultivated bacterial solution to 5.5×10 by ten-fold dilution method with sterile water. 6 pcs L -1 .

[0038] This experiment adopts the soil cultivation pot experiment. The casuarinas selected for the experiment are annual seedlings with an average seedling height of 20cm and an average ground diameter of 3.0mm. They were provided by the Chihu State-owned Forest Farm in Hui'an, Fujian Province. On July 3, 2017, casuarinas with consistent growth were selected and planted in plastic pots with a diameter of 15 cm and a height of 10 cm. The soil required for the experiment was mixed and weighed, and an equal amount (4kg) of yellow core soil was placed in each pot. The nutrient conte...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an endophytic fungus Z1 for increasing the biomass of Casuarina equisetifolia in a high-salt environment, and belongs to the field of microorganisms. The classification name ofthe endophytic fungus Z1 is Botryosphaeria sp., and the endophytic fungus Z1 is preserved in China General Microbiological Culture Collection Center on November 20, 2019 with the preservation numberof CGMCC NO.18817. The bacterial liquid of the endophytic fungus Z1 is applied to planting of Casuarina equisetifolia seedlings in the high-salt environment in a rhizosphere soil watering mode or a seedling direct inoculation mode, and the biomoass of the Casuarina equisetifolia can be increased. A Casuarina equisetifolia-endophytic fungus symbiotic system is built to improve the growth of Casuarina equisetifolia under high-salt stress and provide a data basis and a reference basis for enriching strain information of endophytic fungi of woody plants and manufacturing biological bacterial fertilizers for Casuarina equisetifolia industrial forest operation.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an endophytic fungus Z1 which can increase the biomass of casuarina under high-salt environment. Background technique [0002] Salt stress seriously affects the growth and development of crops and forest plants, and is the main abiotic stress worldwide. Excess salt ions, such as Na + 、K + , Cl - After being ingested by plants, the ion concentration in plant cells will increase, and various biological enzymes in plant cells can only be active in a narrow range of ion concentrations, such as Na + ions and Cl - Ion requirements below 50mM for K + For ions, it is 0.1-0.2M. Therefore, changes in ion concentration affect the activity of biological enzymes, thereby affecting the growth of plants. Salt stress can also damage the plant cell membrane function by affecting a series of changes such as the composition, permeability, and transport of the plasma membra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G17/00A01P21/00C12R1/645
CPCA01G17/005C12N1/145C12R2001/645
Inventor 李键龙凤洪滔林勇明谢安强
Owner FUJIAN AGRI & FORESTRY UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com