Triple inactivated vaccine for pigs and preparation method and application thereof

Abstract

The invention discloses a triple inactivated vaccine for pigs and a preparation method and application of the triple inactivated vaccine. The triple inactivated vaccine for pigs comprises recombinantbaculovirus for expressing an O-type FMDV VP1 gene, swine senecavirus, H3N2 subtype swine influenza virus and an immunologic adjuvant. The invention also discloses a method for preparing the triple inactivated vaccine for pigs. The method comprises the following steps: (1) amplifying and inactivating the recombinant baculovirus for expressing the O-type FMDV VP1 gene; (2) mixing the inactivated recombinant baculovirus fluid for expressing the O-type FMDV VP1 gene, the inactivated swine senecavirus fluid and the H3N2 subtype swine influenza virus to obtain a water phase; (3) heating the immunologic adjuvant to obtain an oil phase; and (4) adding the oil phase into the water phase, mixing and emulsifying. Immune protective efficacy detection results prove that the prepared triple inactivatedvaccine for pigs can effectively prevent and treat O-type foot-and-mouth disease virus, swine senecavirus and H3N2 subtype swine influenza virus simultaneously.

Description

technical field [0001] The invention relates to a triple inactivated vaccine for pigs, in particular to a triple inactivated vaccine for porcine foot-and-mouth disease baculovirus vector, porcine Seneca virus and H3N2 subtype swine influenza virus and a preparation method thereof, belonging to the field of triple inactivated vaccines for pigs. Background technique [0002] Type O FMDV is a member of the genus FMDV and the family Picornaviridae. Among all seven serotypes of foot-and-mouth disease virus, type O is the most prevalent and widely distributed in the world, and has been reported in northern Africa, Eastern Europe, the entire South America and most parts of Asia, seriously threatening the development of global animal husbandry. In the preparation process of inactivated vaccines, due to the disadvantages of high equipment safety requirements, complicated process, high cost, incomplete inactivation of virus strains, and the risk of loose virus, baculovirus vector vacc...

AI Technical Summary

Problems solved by technology

The typical clinical symptoms of pigs infected with S IV are cough, sneeze, runny nose, high fever, loss of appetite, depression, exhaustion, dyspnea, delayed slaughter, reproductive disorders, etc., which are characterized by strong infectivity and high incidence. The mortality rate is low, but it will seriously affect the growth performance of pigs and delay the slaughter time; at the same time, porcine influenza is very easy to mix with other respiratory diseases or secondary infection, and develop into porcine respiratory disease syndrome, which will cause a sudden increase in mortality and give a Pork industry poses great harm

[0005] So far, there is a lack of a triple inactivated vaccine for pigs that can effectively prevent type O foot-and-mouth disease virus, porcine Seneca valley virus and influenza virus at the same time

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