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Phosphate radical colorimetric detection method based on bimetal MOF nano oxidase

A phosphate radical and oxidase technology, applied in the field of analytical chemistry, can solve the problems of complex operation, insufficient anti-interference ability, harsh environmental requirements, etc., and achieve the effects of accurate measurement results, low detection cost, and sensitive response.

Active Publication Date: 2020-04-10
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN106093168A "Phosphate Electrochemical Sensor Based on MEMS Technology and Its Application in Dynamic Detection of Phosphate" discloses a method for electrochemical dynamic detection of phosphate, which can overcome the resistance of existing phosphate ion selective electrode sensors. The disadvantage of insufficient interference ability, minimize the interference of other ions on the target ion, high accuracy, and can be used for rapid detection of phosphate ions such as soil in the field
[0004] However, among the above-mentioned technical solutions, some detection methods are harsh on the environment, relatively high in cost, relatively complicated in operation, and require high reagent storage conditions.

Method used

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  • Phosphate radical colorimetric detection method based on bimetal MOF nano oxidase
  • Phosphate radical colorimetric detection method based on bimetal MOF nano oxidase
  • Phosphate radical colorimetric detection method based on bimetal MOF nano oxidase

Examples

Experimental program
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Effect test

Embodiment 1

[0043] A method for preparing a bimetallic metal-organic framework as an oxidase-like mimic, comprising the following steps:

[0044] 1) 9.1mmol Zr(NO 3 ) 4 , 0.9mmol Ce(NH 4 ) 2 (NO 3 ) 6 , 10mmol terephthalic acid and 2mL acetic acid are thoroughly mixed and dissolved in 15mL DMF;

[0045] 2) The mixed solution was placed in a hydrothermal reactor for 2 h at 80 °C, the product was taken out, rinsed with acetone and ethanol three times, and dried by centrifugation to obtain a bimetallic metal organic framework UiO-66-(Ce / Zr);

[0046] 3) Prepare NaOH (2.5M) and H at a volume ratio of 10:1 2 o 2 (30wt%) preparatory solution, mix evenly for subsequent use;

[0047] 4) Weigh 20 mg of UiO-66-(Ce / Zr) obtained in the previous step into 4.0 mL of deionized water, disperse it evenly by ultrasonication for 20 min, add 50 μL of freshly prepared preparative solution in step 3) to the mixture, and oxidize for 5 min;

[0048] 5) Wash the above complex repeatedly with deionized ...

Embodiment 2

[0053] A method for preparing a bimetallic metal-organic framework as an oxidase-like mimic, comprising the following steps:

[0054] 1) 1.67mmol Zr(NO 3 ) 4 , 8.33mmol Ce(NH 4 ) 2 (NO 3 ) 6 , 10mmol terephthalic acid and 5mL acetic acid are fully mixed and dissolved in 20mL DMF;

[0055] 2) Put the mixed solution in a hydrothermal reactor to react at 120°C for 24 hours, take out the product, wash it with acetone and ethanol three times, and dry it by centrifugation to prepare the bimetallic metal organic framework UiO-66-(Ce / Zr);

[0056] 3) Prepare NaOH (2.5M) and H at a volume ratio of 15:1 2 o 2 (30wt%) preparatory solution, mix evenly for subsequent use;

[0057] 4) Weigh 20 mg of UiO-66-(Ce / Zr) obtained in the previous step into 4.0 mL of deionized water, and disperse it evenly by ultrasonication for 20 min, add 50 μL of freshly prepared preparative solution in step 3) to the mixed solution, and oxidize for 20 min;

[0058] 5) Wash the above complex repeatedly...

Embodiment 3

[0063] A method for preparing a bimetallic metal-organic framework as an oxidase-like mimic, comprising the following steps:

[0064] 1) Add 0.9mmol Zr(NO 3 ) 4 , 9.1mmol Ce(NH 4 ) 2 (NO 3 ) 6 , 10mmol terephthalic acid and 8mL acetic acid are fully mixed and dissolved in 20mL DMF;

[0065] 2) The mixed solution was placed in a hydrothermal reactor for 48 hours at 150°C, the product was taken out, rinsed with acetone and ethanol three times, and dried by centrifugation to obtain a bimetallic metal organic framework UiO-66-(Ce / Zr);

[0066] 3) Prepare NaOH (2.5M) and H at a volume ratio of 20:1 2 o 2 (30wt%) preparatory solution, mix evenly for subsequent use;

[0067] 4) Weigh 20 mg of UiO-66-(Ce / Zr) obtained in the previous step in 4.0 mL of deionized water, ultrasonically disperse it for 20 min to make it evenly dispersed, add 50 μL of freshly prepared preparative solution in step 3) to the mixed solution, and oxidize for 60 min;

[0068] 5) Wash the above complex...

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Abstract

The invention belongs to the technical field of analytical chemistry, and relates to a phosphate radical detection method, in particular to a phosphate radical colorimetric detection method based on bimetal MOF nano oxidase. Firstly, an ultraviolet absorption spectrum standard working curve is drawn, and then the phosphate radical content of a to-be-detected object is determined through the working curve. The invention further discloses a preparation method of the bimetallic (Ce / Zr) organic framework structure (MOFs) nano oxidase, namely Treated-UiO-66-(Ce / Zr). The method has the advantages ofmild conditions in the detection process, no need of other reagents, realization of convenient and rapid detection of phosphate radicals, low detection cost and simple operation; phosphate radicals are detected through a dual-mode calibration TMB / ABS + Treated-UiO-66-(Ce / Zr) system, the detection limit is as low as 14 nM, and the detectable range is as wide as 0.667-667 [mu] M; phosphate radicalsare detected by using a TMB / ABTS + Treated-UiO-66-(Ce / Zr) system, the measurement result of an actual sample is accurate, the response to the phosphate radicals is sensitive, and the detection of thephosphate radicals in the environmental drinking water can be realized.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and relates to a method for detecting phosphate radicals, in particular to a colorimetric detection method for phosphate radicals based on bimetallic MOF nano-oxidases. Background technique [0002] Phosphate is one of the nutrients needed by the human body. The normal content of phosphate in the human body is generally 2.5-4.5mg / dL. Excessive intake of phosphate will endanger the safety of blood vessels, induce various diseases, and affect health. Phosphate in the environment widely exists in drinking water, mainly from the sewage and wastewater discharge in the food industry, detergent manufacturing industry and pesticide production. In addition, too much phosphate in the environment will also lead to eutrophication of the water body, which is not conducive to the growth of aquatic organisms. Therefore, accurate and rapid detection of phosphate content in environmental water has be...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/78
CPCG01N21/33G01N21/78
Inventor 李欣牛湘衡刘朋
Owner JIANGSU UNIV
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