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Preparation method of electrospun fiber scaffold material

An electrospinning and fiber scaffold technology, applied in the field of medical materials, can solve the problems of small pore size and poor degradation performance of materials, and achieve the effects of increasing the scaffold space, enhancing the potential, and broadening the scope of the space.

Active Publication Date: 2021-10-19
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: The present invention provides an electrospun fiber scaffold material and its preparation method, which solves the problems of small pore size and poor degradation performance of existing materials

Method used

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  • Preparation method of electrospun fiber scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Step 1. Preparation of basement membrane and stirring and crushing:

[0032] S1, preparing a polylactic acid-glycolic acid copolymer electrospinning chloroform solution with a mass percentage of 2%, and making a nanofiber film with a three-dimensional structure (also called electrospinning) by electrospinning the electrospinning solution fiber membrane), the electrospinning conditions are: voltage 5kV, receiving distance 6cm, sample injection speed 0.5mL / h;

[0033] S2. Preliminary experiment: Scissors cut 4g poly(lactic acid-glycolic acid copolymer) electrospun fiber membrane, add it to the centrifuge tube according to the design, and add Tris-HCl solution;

[0034] S3. Stir with a high-speed stirrer in the centrifuge tube (control the stirring speed to 1200r / h, stir for 2 minutes), stir and smash until completely slurryed.

[0035] Step 2, preparation of cross-linking solution:

[0036] S1. Prepare a PEI-dopamine solution with a weight ratio of 1.8% (the weight rati...

Embodiment 2

[0043] About PEI Concentration

[0044] Cross-linking electrospun fiber membranes with different concentrations of PEI-dopamine solutions

[0045] Step 1. Preparation of basement membrane and stirring and crushing:

[0046] S1, preparing a polylactic acid-glycolic acid copolymer electrospinning dimethylformamide solution with a mass percentage of 3%, and making the electrospinning solution into a nanofiber film with a three-dimensional structure (also known as Electrospun fiber membrane), the electrospinning conditions are: voltage 13kV, receiving distance 12cm, sample injection speed 2mL / h;

[0047] S2. Preliminary experiment: cut 3-5g electrospun fiber membrane with scissors, put it into a centrifuge tube according to the design, and add Tris-HCl solution.

[0048] S3. Stir with a high-speed mixer in a centrifuge tube (control the stirring speed to 1000r / h, and stir for 4 minutes), stir and crush until completely slurryed.

[0049] Step 2, preparation of cross-linking solut...

Embodiment 3

[0058] About PEI Crosslinking Sequence

[0059] Step 1, preparation of 5% polylactic acid-glycolic acid copolymer (PLGA) and polycaprolactone (PCL) electrospinning trifluoroethanol solution, and the electrospinning solution is made by electrospinning Nanofiber film with three-dimensional structure (also known as electrospun fiber film), the electrospinning conditions are: voltage 19kV, receiving distance 20cm, sample injection speed 3.5mL / h;

[0060] Break 3-5g of poly(lactic-co-glycolic acid) and polycaprolactone (PCL) film in Tris-Hcl (pH=8.5%) solution (control the stirring speed to be 1200r / h), stir for 2 minutes, until broken into pulp.

[0061] Step 2, preparing 0.5% PEI-dopamine (DA) cross-linking solution:

[0062] S1. Measure 10ml Tris-Hcl into 2 watch glasses, respectively add 2 parts of 0.02g dopamine into the watch glasses, and dissolve completely.

[0063] S2. Weigh 0.05g of PEI into the watch glass and dissolve it completely.

[0064] Step 3: Add the cross-li...

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Abstract

The invention discloses a method for preparing an electrospinning fiber support material. The electrospun membrane of a degradable polymer material is used as a base material, and the prepared electrospun fibers are crushed by a high-speed disperser, and then crosslinked by a biological crosslinking agent. and freeze-drying to prepare a scaffold material in the body with a suitable porosity. The invention provides a method for extending the application of the electrospun nanofiber material, which can realize the enlargement of the three-dimensional pore size, and can regulate cell adhesion and promote expression.

Description

technical field [0001] The invention relates to the technical field of medical materials, and mainly relates to a preparation method of an electrospinning fiber support material. Background technique [0002] An ideal bone tissue engineering scaffold material can have good biocompatibility, can promote tissue regeneration, provide enough three-dimensional space for the survival of cells, and can effectively deliver nutrients; it can be degraded, can be effectively absorbed by the human body, and will not cause inflammation reaction. In addition, it should be able to facilitate the attachment and proliferation of cells and induce bone regeneration. Commonly used scaffold materials are high-scoring materials, which are divided into natural and synthetic. Naturals are natural biological materials that can be found in nature, such as collagen. Collagen has been widely used as scaffold material and drug delivery carrier because of its good biocompatibility. However, while nat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/58A61L27/56
CPCA61L27/26A61L27/56A61L27/58A61L2430/02C08L67/04C08L79/02C08L89/00
Inventor 周雪锋成希顾宁
Owner SOUTHEAST UNIV
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