Method for preparation of ganoderma lucidum enzyme through continuous fermentation
A Ganoderma lucidum and enzyme technology, which is applied in the field of continuous fermentation to prepare Ganoderma lucidum enzymes, can solve the problems of spoilage bacteria, the inability to completely guarantee the absence of pathogenic bacteria, and the retention of active components of enzymes, so as to enhance the antioxidant effect, ensure antioxidant activity, and inhibit overgrowth. The effect of oxidation
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Embodiment 1
[0032] A method for preparing ganoderma enzymes by continuous fermentation, specifically comprising the steps of:
[0033] S1. Ganoderma pretreatment: place Ganoderma lucidum in a drying oven to dry at 40°C; after drying, crush Ganoderma lucidum and sieve it to obtain dry Ganoderma lucidum powder; add deionized water and a degreasing agent to the dried Ganoderma lucidum powder, After the degreasing treatment, the lipids are removed by filter membrane filtration, and finally the Ganoderma lucidum powder is dried again at 40° C.; the degreasing agent is a mixture of alkali metal hydroxide and alkali metal carbonate;
[0034] S2. Preparation of ganoderma lucidum extract: add dilute alkali solution to the dried ganoderma lucidum powder for initial extraction, the extraction temperature is controlled at 50°C, and ultrasonic treatment is performed for 30 minutes; after ultrasonic treatment, acetic acid is used to adjust the pH of the extraction solution to neutral, Add the modified ...
Embodiment 2
[0043] A method for preparing ganoderma enzymes by continuous fermentation, specifically comprising the steps of:
[0044] S1. Ganoderma pretreatment: place Ganoderma lucidum in a drying oven to dry at 50°C; after drying, crush Ganoderma lucidum and sieve it to obtain dry Ganoderma lucidum powder; add deionized water and a degreasing agent to the dried Ganoderma lucidum powder, After the degreasing treatment, the lipids are removed by filter membrane filtration, and finally the Ganoderma lucidum powder is dried again at 50° C.; the degreasing agent is alkali metal bicarbonate;
[0045] S2. Preparation of ganoderma lucidum extract: add dilute alkali solution to the dried ganoderma lucidum powder for initial extraction, the extraction temperature is controlled at 60°C, and ultrasonic treatment is performed for 35 minutes; after ultrasonic treatment, acetic acid is used to adjust the pH of the extraction solution to neutral, Add the modified extractant and the leaching solution a...
Embodiment 3
[0054] A method for preparing ganoderma enzymes by continuous fermentation, specifically comprising the steps of:
[0055] S1. Ganoderma pretreatment: place Ganoderma lucidum in a drying oven to dry at 45°C; after drying, crush Ganoderma lucidum and sieve it to obtain dry Ganoderma lucidum powder; add deionized water and a degreasing agent to the dried Ganoderma lucidum powder, After the degreasing treatment, filter to remove the lipids, and finally dry the Ganoderma lucidum powder again at 45° C.; the degreasing agent is alkali metal hydroxide;
[0056] S2. Preparation of ganoderma lucidum extract: add dilute alkali solution to the dried ganoderma lucidum powder for initial extraction, the extraction temperature is controlled at 55° C., and ultrasonic treatment is performed for 40 minutes; after ultrasonic treatment, acetic acid is used to adjust the pH of the extraction solution to neutral, Add the modified extractant and the leaching solution and mix thoroughly for secondar...
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