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Method for preparation of ganoderma lucidum enzyme through continuous fermentation

A Ganoderma lucidum and enzyme technology, which is applied in the field of continuous fermentation to prepare Ganoderma lucidum enzymes, can solve the problems of spoilage bacteria, the inability to completely guarantee the absence of pathogenic bacteria, and the retention of active components of enzymes, so as to enhance the antioxidant effect, ensure antioxidant activity, and inhibit overgrowth. The effect of oxidation

Inactive Publication Date: 2020-04-10
武夷山元生泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the existing Ganoderma lucidum enzyme production process is relatively simple, and the production cycle is long, there are the following disadvantages: First, the fermentation process of the enzyme needs to change the fermenter or multiple transfers and inoculations, which is very difficult in the process. It is easy to cause the growth of spoilage bacteria or the pollution of external bacteria, because most of them are fermented by various microorganisms in the air. Even though the finished product is enzyme, it cannot completely guarantee that there is no pollution of pathogenic bacteria, and it is easy to cause food damage due to ingestion. poisoning; the second is the unified extraction and fermentation process control, which cannot fully retain the activity of different enzyme active ingredients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for preparing ganoderma enzymes by continuous fermentation, specifically comprising the steps of:

[0033] S1. Ganoderma pretreatment: place Ganoderma lucidum in a drying oven to dry at 40°C; after drying, crush Ganoderma lucidum and sieve it to obtain dry Ganoderma lucidum powder; add deionized water and a degreasing agent to the dried Ganoderma lucidum powder, After the degreasing treatment, the lipids are removed by filter membrane filtration, and finally the Ganoderma lucidum powder is dried again at 40° C.; the degreasing agent is a mixture of alkali metal hydroxide and alkali metal carbonate;

[0034] S2. Preparation of ganoderma lucidum extract: add dilute alkali solution to the dried ganoderma lucidum powder for initial extraction, the extraction temperature is controlled at 50°C, and ultrasonic treatment is performed for 30 minutes; after ultrasonic treatment, acetic acid is used to adjust the pH of the extraction solution to neutral, Add the modified ...

Embodiment 2

[0043] A method for preparing ganoderma enzymes by continuous fermentation, specifically comprising the steps of:

[0044] S1. Ganoderma pretreatment: place Ganoderma lucidum in a drying oven to dry at 50°C; after drying, crush Ganoderma lucidum and sieve it to obtain dry Ganoderma lucidum powder; add deionized water and a degreasing agent to the dried Ganoderma lucidum powder, After the degreasing treatment, the lipids are removed by filter membrane filtration, and finally the Ganoderma lucidum powder is dried again at 50° C.; the degreasing agent is alkali metal bicarbonate;

[0045] S2. Preparation of ganoderma lucidum extract: add dilute alkali solution to the dried ganoderma lucidum powder for initial extraction, the extraction temperature is controlled at 60°C, and ultrasonic treatment is performed for 35 minutes; after ultrasonic treatment, acetic acid is used to adjust the pH of the extraction solution to neutral, Add the modified extractant and the leaching solution a...

Embodiment 3

[0054] A method for preparing ganoderma enzymes by continuous fermentation, specifically comprising the steps of:

[0055] S1. Ganoderma pretreatment: place Ganoderma lucidum in a drying oven to dry at 45°C; after drying, crush Ganoderma lucidum and sieve it to obtain dry Ganoderma lucidum powder; add deionized water and a degreasing agent to the dried Ganoderma lucidum powder, After the degreasing treatment, filter to remove the lipids, and finally dry the Ganoderma lucidum powder again at 45° C.; the degreasing agent is alkali metal hydroxide;

[0056] S2. Preparation of ganoderma lucidum extract: add dilute alkali solution to the dried ganoderma lucidum powder for initial extraction, the extraction temperature is controlled at 55° C., and ultrasonic treatment is performed for 40 minutes; after ultrasonic treatment, acetic acid is used to adjust the pH of the extraction solution to neutral, Add the modified extractant and the leaching solution and mix thoroughly for secondar...

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Abstract

The invention relates to a method for preparation of ganoderma lucidum enzyme through continuous fermentation. The method specifically includes the steps of: S1, ganoderma lucidum pretreatment; S2, preparation of a ganoderma lucidum extracted solution; S3, primary fermentation; S4, secondary fermentation; and S5, continuous fermentation. A ganoderma lucidum extracted solution is prepared before aganoderma lucidum enzyme fermentation process, active ingredients contained in ganoderma lucidum are fully extracted, continuous fermentation is adopted in the fermentation process, the fermentation time is effectively shortened, the risk of sundry fungus contamination in the existing fermentation process is avoided, and finally the ganoderma lucidum enzyme with high content of ganoderma lucidum polysaccharide, ganoderic acid, ganoderma lucidum organic germanium and other active ingredients is prepared.

Description

technical field [0001] The invention belongs to the technical field of health product processing, and in particular relates to a method for preparing ganoderma lucidum enzyme by continuous fermentation. Background technique [0002] Ganoderma lucidum has been hailed as the "auspicious grass" by Chinese medical scientists in the past dynasties. There have been a lot of research on the pharmacological effects of Ganoderma lucidum, which shows that Ganoderma lucidum has a wide range of pharmacological effects, such as anti-tumor effect, immune regulation effect, anti-radiation and anti-chemotherapy Function, sedative and tranquilizing effect, strong heart and anti-myocardial ischemia effect, blood lipid regulation effect, hypoglycemic effect, anti-asthma effect, liver protection effect, anti-hypoxia and anti-aging effect, etc. drink in. [0003] The value of Ganoderma lucidum is that it is rich in polysaccharides, triterpenoids, adenosine and germanium elements. Wild Ganoderma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00
CPCA23L33/00
Inventor 毛景华张泰顺黄春花黄敏
Owner 武夷山元生泰生物科技有限公司
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