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Substrate for biochemical calibrator and biochemical calibrator

A calibrator, biochemical technique

Pending Publication Date: 2020-04-03
NINGBO RUI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a matrix for biochemical calibration products, which aims to solve the problem that the existing calibration products with serum as the matrix still have large differences in activity loss during the freeze-drying process, and the freeze-dried products are not stable during freeze-drying storage. Technical problems that are stable and affect the accurate results of subsequent testing

Method used

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  • Substrate for biochemical calibrator and biochemical calibrator
  • Substrate for biochemical calibrator and biochemical calibrator
  • Substrate for biochemical calibrator and biochemical calibrator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Configure biochemical calibrator matrix:

[0032] Add 10 mmol of sodium hydrogen phosphate and 10 mmol of sodium dihydrogen phosphate to 500 mL of distilled water to prepare a buffer;

[0033] Add 2 g of sucrose, 2 g of β-glucan, 2 g of human serum albumin, 0.5 g of betaine, 5 g of glycine, 0.02 mmol of sodium D-isoascorbate, and 0.05 mmol of benzyl to the buffer in sequence Sulfonyl fluoride, 0.05 mmol serine protease inhibitors, 5 g tert-butanol, 0.2 g sodium azide, 8.5 g sodium chloride and 0.1 mmol magnesium chloride and 0.1 mmol zinc chloride;

[0034] Dilute the buffer solution to 1 L to obtain the matrix for biochemical calibration products.

[0035] (2) Configuration of biochemical calibrator (lyophilized powder):

[0036] Add 100 U of alkaline phosphatase, 100 U of α-amylase, 100 U of alanine aminotransferase, 100 U of pancreatic amylase, 100 U of aspartate aminotransferase, 20 U of Aspartate aminotransferase mitochondrial isozyme, 200U creatine kinase, ...

Embodiment 2

[0040] (1) Configure biochemical standard matrix:

[0041] Add 100 mmol of sodium hydrogen phosphate and 100 mmol of sodium dihydrogen phosphate to 500 mL of distilled water to prepare a buffer;

[0042] Add 50 g sucrose, 100 g β-glucan, 50 g human serum albumin, 5 g betaine, 20 mmol glycine, 50 mmol D-sodium erythorbate, 2 mmol benzyl to the buffer in sequence Sulfonyl fluoride, 2 mmol serine protease inhibitors, 30 g tert-butanol, 0.5 g sodium azide, 9.5 g sodium chloride and 5 mmol magnesium chloride and 5 mmol zinc chloride;

[0043] Dilute the buffer solution to 1 L to obtain the matrix for biochemical calibration products.

[0044] (2) Configuration of biochemical calibrator (lyophilized powder):

[0045] Add 100 U of alkaline phosphatase, 100 U of α-amylase, 100 U of alanine aminotransferase, 100 U of pancreatic amylase, 100 U of aspartate aminotransferase, 20 U of Aspartate aminotransferase mitochondrial isozyme, 200U creatine kinase, 50U creatine kinase MB isozyme,...

Embodiment 3

[0048] (1) Configure biochemical calibrator matrix:

[0049] Add 30 mmol potassium hydrogen phosphate and 30 mmol potassium dihydrogen phosphate to 500 mL distilled water to prepare a buffer;

[0050] Add 10 g lactose, 10 g polyvinylpyrrolidone, 20 g bovine serum albumin, 2 g triton X-100, 10 mmol glycine, 20 mmol vitamin E, 0.1 mmol phenylmethylsulfonate in sequence to the buffer Acyl fluoride, 10 grams of tert-butanol, 0.2 grams of sodium azide, 8.5 grams of sodium chloride and 1 mmol of magnesium chloride;

[0051] Dilute the buffer solution to 1 L to obtain the matrix for biochemical calibration products.

[0052] (2) Configuration of biochemical calibrator (lyophilized powder):

[0053] Add 300U of alkaline phosphatase, 200U of α-amylase, 200U of alanine aminotransferase, 200U of pancreatic amylase, 200U of aspartate aminotransferase, 100U of Aspartate aminotransferase mitochondrial isozyme, 500U creatine kinase, 150U creatine kinase MB isozyme, 7000U cholinesterase, 1...

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Abstract

The invention is applicable to the technical field of biochemistry, in particular to a calibrator for a biochemical calibrator and the biochemical calibrator. Each liter of the matrix for the biochemical calibrator comprises the following components of 10 to 100 mmol of a pH regulator, 2-50 g of a saccharide protective agent, 2-100 g of a polymer protective agent, 5-50 g of a protein protective agent, 0.5 to 5 grams of surfactant, 5 to 20 millimoles of amino acid, 0.02 to 50 millimoles of antioxidant, 0.05 to 2 millimoles of protease inhibitor, 5 to 30 grams of alcohol organic solvent, 0.2 to0.5 gram of preservative, 8.5 to 9.5 grams of sodium chloride and 0.1 to 5 millimoles of magnesium salt and / or zinc salt. According to the calibrator for the biochemical calibrator, when the analysiscomponents are added into the matrix for the biochemical calibrator, the components in the matrix for the biochemical calibrator can play a better role in protecting the analysis components, particularly, the structural variation caused by water loss of the analysis components in the freeze-drying process can be avoided, and the effect of the analysis components is effectively reserved.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a matrix for a biochemical calibrator and a biochemical calibrator. Background technique [0002] In clinical diagnosis, in order to facilitate the diagnosis of samples, calibrator is usually required. Calibrator can contain one or even dozens of analytical components, and its quality is directly related to the accuracy and reliability of the test results. At present, commonly used calibrators are in liquid and freeze-dried powder forms. Liquid calibrators are easy to use, but have poor stability, especially biochemical calibrators containing enzymes, which are easily inactivated when stored in solution and have a short shelf life. Therefore, biochemical calibrators are usually stored in a freeze-dried manner. [0003] In the existing freeze-dried powder, the analytical components are usually dissolved in the serum matrix first, and then freeze-dried. Since so...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N33/48
CPCG01N1/28G01N33/48G01N2001/2893
Inventor 王超儿陈媛江虹张闻周海滨
Owner NINGBO RUI BIO TECH
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