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Determination method of α-solanine and α-chaconine in a kind of potato and its products

A determination method and potato technology, which are applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to achieve the best water absorption capacity, unsatisfactory extraction effect, and difficulty in popularization, etc. Destruction, high extraction effect

Active Publication Date: 2022-06-28
酒泉市食品检验检测中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of scientific researchers at home and abroad have studied the extraction and detection of solanin. At present, the common extraction methods include leaching, Soxhlet extraction, reflux extraction, ultrasonic extraction, microwave extraction, and organic reagent extraction. Common detection methods include liquid phase method and liquid-mass method, but in the actual application process, there are problems such as difficult extraction, poor peak shape, and large detection error; in July 2018, the State Administration for Market Regulation issued, Supplementary test method for the determination of α-solanine and α-chaconine in potatoes and their products, the extraction and purification process of α-solanine and α-chaconine use acidified acetonitrile, anhydrous magnesium sulfate and anhydrous sodium acetate, After the combination of the three reagents, the water absorption capacity of the sample cannot reach the best state, that is, the water content in the sample has not yet reached the minimum, resulting in unsatisfactory extraction effects of α-solanine and α-chaconine. At the same time, the detection method uses liquid phase Chromatography-tandem mass spectrometer is relatively expensive, and its operation and maintenance costs in the later stage are 3 to 5 times that of liquid chromatography. It is difficult to popularize this supplementary method in grassroots inspection institutions. Therefore, it is urgent to establish a A method for the detection of α-solanine and α-chaconine in potato meat with complete extraction, simple processing, and easy generalization

Method used

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  • Determination method of α-solanine and α-chaconine in a kind of potato and its products
  • Determination method of α-solanine and α-chaconine in a kind of potato and its products
  • Determination method of α-solanine and α-chaconine in a kind of potato and its products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: the assay method of α-solanine and α-chaconine in potato bud, green skin

[0044] (1) Preparation of α-solanine and α-chaconine test solution

[0045] Accurately weigh 100g of potato sprouts and green skins, and use a homogenizer (IKA T10basic) to thoroughly break and mix them to obtain a sample homogenate, then put the sample homogenate into a sub-packaging container, and place it at -20°C Store under refrigeration; accurately weigh 1.000g sample homogenate, put it in a 50ml stoppered quantitative centrifuge tube, add 3.00ml distilled water to the stoppered quantitative centrifuge tube, use a vortex mixer (IKA MS3) to vortex mix for 2min , add 25.0ml acidified methanol (1% formic acid+99% methanol), then use a vortex mixer (IKA MS3) to vortex mix for 1min; add 1.0g anhydrous sodium sulfate, 1.0g of anhydrous magnesium acetate, capped and manually shaken vigorously for 1min, then vortexed and mixed with a vortex mixer (IKA MS3) for 1min, put the stoppered...

Embodiment 2

[0066] Embodiment 2: the assay method of α-solanine and α-chaconine in potato meat and its products

[0067] (1) Preparation of α-solanine and α-chaconine test solution

[0068] Accurately weigh 100g of potato meat, use a homogenizer (IKA T10basic) to fully break and mix it to obtain a sample homogenate, then put the sample homogenate into a packaging container, and store it in a freezer at -20°C ; Accurately weigh 2.000g sample homogenate, place in a 50ml stopper quantitative centrifuge tube, add 6.00ml distilled water to the stopper quantitative centrifuge tube, use a vortex mixer (IKA MS3) to vortex mix for 2min, add 25.0 ml acidified methanol (1% formic acid+99% methanol), then use a vortex mixer (IKA MS3) to vortex mix for 1 min; add 2.0 g of anhydrous sodium sulfate, 1.0 g of anhydrous Magnesium acetate in water, capped and manually shaken vigorously for 1min, then vortexed and mixed with a vortex mixer (IKA MS3) for 1min, put the stoppered quantitative centrifuge tube ...

Embodiment 3

[0089] Example 3: Using different solvents and water-absorbing agents to extract and compare the content of α-solanine and α-chaconine in potato sprouts

[0090] After the potato sprouts were homogenized, six samples of the same amount were weighed and numbered ①②③④⑤⑥⑥ respectively. According to the method described in Example 1, 6 different α-solanine and α-chaconine solvents and water-absorbing agents were used for each sample. α-solanine and α-chaconine are extracted in the sample, and each sample carries out α-solanine and α-chaconine according to method step (2), (3) liquid chromatography described in embodiment 1 The detection of the alkali content obtained the comparison results of the extraction effects of different solvents and water-absorbing agents on α-solanine and α-chaconine, as shown in Table 3:

[0091] Table 3 Comparison of extraction effects of different solvents and water-absorbing agents on α-solanine and α-chaconine in potato sprout samples

[0092]

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Abstract

The invention discloses a method for determining α-solanine and α-chaconine in potatoes and their products. The steps mainly include: preparing the test solution of α-solanine and α-chaconine, drawing a standard curve, obtaining As a result of sample testing, the present invention measures the content of α-solanine and α-chaconine in potatoes and their products by adopting a liquid chromatograph that is easy to promote and popularize in the grass-roots inspection institutions, and the detection method is connected in series with liquid chromatography Compared with the detection results of the mass spectrometer method, the relative deviation is less than 10%. The detection method is easy to operate and has high accuracy. It can be widely used in grassroots inspection institutions, which greatly facilitates grassroots inspection and testing institutions.

Description

technical field [0001] The invention relates to the field of detection of α-solanine and α-chaconine, in particular to a method for determining α-solanine and α-chaconine in potatoes and products thereof. Background technique [0002] Potatoes, also known as potatoes and yams, are the fourth largest food crops after rice, wheat, and corn. Potatoes, like other Solanaceae plants, generally contain a class of glycoside derivatives of steroidal alkaloids in plants and tubers, commonly known as dragons. The substance of solanine, its main components are α-solanine and α-chaconine, solanine is a kind of toxic alkaloid, usually, the content is low, it will not affect its edible quality, but when When potatoes germinate and turn green due to improper storage, the content of solanine will increase significantly. If humans and animals consume 0.2g, poisoning symptoms will appear, and severe cases may cause death. Potato consumption is huge, but there are many problems in its detectio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8637
Inventor 任兴权刘盼苏菊王蓉曾文锦周丽史蓉李婷婷段婷
Owner 酒泉市食品检验检测中心
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