Urine 5-hydroxyindoleacetic acid detection reagent and preparation method thereof
A technology of oxindole acetic acid and detection reagent, which is applied to measurement devices, material analysis, instruments and other directions by observing the influence on chemical indicators, can solve the problems of complex urine composition, false positives, misdiagnosis, etc., and achieves sensitivity and High accuracy, preventing false positives, and strong specificity
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Embodiment 1
[0030] The present embodiment provides a urine 5-oxindoleacetic acid detection reagent, which is an aqueous solution comprising nitric acid, phosphoric acid, mercuric nitrate, mercurous nitrate, zinc nitrate, phosphomolybdic acid and silicotungstic acid, wherein the concentration of mercuric nitrate is 0.12mol / L, the concentration of mercurous nitrate is 0.75mol / L, the concentration of zinc nitrate is 0.36mmol / L, the concentration of phosphomolybdic acid is 0.75mmol / L, and the concentration of silicotungstic acid is 4.17mmol / L.
[0031] The preparation method of the above-mentioned detection reagent comprises the following steps:
[0032] S1. Preparation of mixed acid solution: mix concentrated nitric acid and concentrated phosphoric acid in equal volumes, add water to dilute to 3 times the volume of the mixture, and cool to room temperature for later use;
[0033] S2, preparation of A solution: take mercuric nitrate and dissolve it in the mixed acid solution described in step...
Embodiment 2
[0040] The present embodiment provides a urine 5-oxindoleacetic acid detection reagent, which is an aqueous solution comprising nitric acid, phosphoric acid, mercuric nitrate, mercurous nitrate, zinc nitrate, phosphomolybdic acid and silicotungstic acid, wherein the concentration of mercuric nitrate is 0.31mol / L, the concentration of mercurous nitrate is 0.56mol / L, the concentration of zinc nitrate is 1.27mmol / L, the concentration of phosphomolybdic acid is 2.81mmol / L, and the concentration of silicotungstic acid is 7.82mmol / L.
[0041] The preparation method of the above-mentioned detection reagent comprises the following steps:
[0042] S1. Preparation of mixed acid solution: mix concentrated nitric acid and concentrated phosphoric acid in equal volumes, add water to dilute to 4 times the volume of the mixture, and cool to room temperature for later use;
[0043] S2, the preparation of A solution: take by weighing mercuric nitrate and dissolve in the mixed acid solution desc...
Embodiment 3
[0050] The present embodiment provides a urine 5-oxindoleacetic acid detection reagent, which is an aqueous solution comprising nitric acid, phosphoric acid, mercuric nitrate, mercurous nitrate, zinc nitrate, phosphomolybdic acid and silicotungstic acid, wherein the concentration of mercuric nitrate is 0.20mol / L, the concentration of mercurous nitrate is 1.07mol / L, the concentration of zinc nitrate is 0.25mmol / L, the concentration of phosphomolybdic acid is 1.46mmol / L, and the concentration of silicotungstic acid is 5.70mmol / L.
[0051] The preparation method of the above-mentioned detection reagent comprises the following steps:
[0052] S1. Preparation of mixed acid solution: mix equal volumes of concentrated nitric acid and concentrated phosphoric acid, add water to dilute to 5 times the volume of the mixture, and cool to room temperature for later use;
[0053] S2, preparation of A solution: take mercuric nitrate and dissolve in the mixed acid solution described in step S1...
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