Method for identifying self-compatibility of diploid potato

Abstract

The invention provides a method for identifying whether a diploid potato is self-compatible, and relates to the technical field of genetic breeding. The method is to identify whether StSCI gene in thediploid potato is transcribed and expressed. The invention also discloses a method for adopting molecular markers to identify whether the StSCI gene is expressed and for screening molecular markers.The method for screening molecular markers comprises: obtaining genomic sequence information of a parent material, screening differential sites of the parent material, screening molecular markers, andidentifying whether the screened molecular markers are available. Identification of self-compatibility of diploid potatoes by adopting the screened molecular markers has the advantages that the identification workload is small, a lot of time is saved, and the identification results are not affected by the environment, are accurate and reliable.

Description

technical field [0001] The invention relates to the technical field of genetic breeding, in particular to a method for identifying whether diploid potatoes are self-compatible, a method for screening molecular markers for identifying whether diploid potatoes are self-compatible, reagents, kits and identification method. Background technique [0002] During diploid potato breeding, diploid potatoes are often self-incompatible, and when pollen from itself or the same S haplotype as the plant falls on the stigma, the pollen tube cannot extend all the way to ovule completion The fertilization process, therefore pollen identical to its own S haplotype is incapable of producing seeds in diploid potato plants. In order to achieve seed breeding in diploid potato breeding, some studies have focused on modifying the self-incompatibility defect of diploid potato through gene editing. [0003] But the identification of self-compatible diploid potato materials has been difficult. At p...

AI Technical Summary

Problems solved by technology

However, the fruit setting of the plant is affected by various internal and external factors, such as low pollen activity due to immature pollen development, high or low temperature during pollination will affect the fruit setting of the plant, and potato, as a flowering plant, may be caused by wind blowing or tweezers not cleaned during pollination. The result of cross-fertilization between adjacent plants caused by cross-fertilization. Therefore, no fruit set or a small number of fruit set in a single year cannot accurately represent the self-compatibility phenotype of the plant. Repeated identification in multiple locations and years is required. Therefore, traditional self-cultivation The identification method of cross-compatibility phenotype is time-consuming, labor-intensive and inaccurate

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