Rapid modulation fluorescence polarization microscopic imaging device and method based on liquid crystal

Abstract

The invention discloses a rapid modulation fluorescence polarization microscopic imaging device and method based on liquid crystal, and relates to the technical field of optical imaging. The device comprises a light source, an excitation filter, a liquid crystal polarization rotator, a dichroscope, an objective lens, an emission filter, a wave plate, an analyzer, an imaging lens, a camera, a liquid crystal controller and a computer. The method comprises the following steps: converting an emergent light beam of the light source into linearly polarized light through a liquid crystal polarizationrotator; after the linear polarization exciting light enters a sample, exciting the fluorescent light; enabling the fluorescent light to pass through the objective lens, the emission filter, the waveplate and the analyzer and then enter the camera target surface for imaging; in the process, enabling the computer to control the liquid crystal controller to drive the liquid crystal polarization rotator to modulate the polarization angle of the incident exciting light, controlling the camera to acquire a series of fluorescence polarization images, and then performing calculating according to the series of fluorescence polarization images to obtain each fluorescence parameter image. The device has the advantages of high imaging speed, high resolution, wide field of view, high modulation precision and the like, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of optical imaging, in particular to a liquid crystal-based rapid modulation fluorescence polarization microscopy imaging device and method. Background technique [0002] Fluorescence microscopy is one of the most commonly used optical measurement methods and is widely used in life sciences, nanotechnology, etc. . Fluorescence has multiple characteristic parameters, including spectrum, intensity, lifetime, polarization, etc. Most fluorescence imaging methods measure fluorescence intensity parameters, and obtain information such as the shape, size, and quantity of specific fluorescently labeled targets through fluorescence intensity. Fluorescence polarization is another important physical quantity. When linearly polarized light is used to excite fluorescent protein-labeled biological staining samples, the fluorescent molecules whose spatial dipole orientation is parallel to the polarization angle of the inci...

AI Technical Summary

Problems solved by technology

Among them, the resolution of ordinary wide-field fluorescence microscopy imaging technology is low; laser scanning confocal technology and two-photon fluorescence microscopy imaging technology use point scanning, and the imaging speed is relatively slow; the imaging system of structured illumination microscopy imaging technology is relatively complex and difficult. high cost

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