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Method for comparative analysis of sheep mycoplasma ovipneumoniae virulence

A technique for comparative analysis of Mycoplasma pneumoniae, applied in the direction of microbial-based methods, analytical materials, biological material analysis, etc., can solve the problem of long experimental period (generally 21-28 days or even longer, expensive experimental animals and sheep, and complicated experimental operations) and other problems, to overcome the effect of relatively expensive price, short test cycle and good repeatability

Active Publication Date: 2020-02-28
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, there are high requirements for experimental animals (healthy sheep or goats, both negative for Mycoplasma ovis pneumoniae pathogen and antibody), long experimental period (generally 21-28 days or even longer), relatively expensive experimental animal sheep, high feeding requirements, and experimental Disadvantages such as complicated operation

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  • Method for comparative analysis of sheep mycoplasma ovipneumoniae virulence

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Embodiment Construction

[0013] The following will be described in conjunction with the embodiments of the present invention.

[0014] For the method for comparing the virulence of ovine mycoplasma pneumoniae, the steps are as follows:

[0015] 1. Prepare 7-day-old healthy SPF chicken embryos (SPF eggs were purchased from Shandong Haotai Experimental Animal Breeding Co., Ltd.), incubate in an incubator at a temperature of 37.0-37.5°C and a humidity of 50%. Fertile eggs, dead and weak embryos.

[0016] 2. Preparation of Mycoplasma pneumoniae bacteria liquid: resuscitate Mycoplasma pneumoniae Y98 strain (purchased from China Veterinary Microbiological Culture Collection Center CVCC, No. CVCC384), MoGH3-3 strain (isolated and preserved by Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences), and inoculate sheep respectively. Mycoplasma pneumoniae medium (improved Thiaucourt's medium), cultured at 37°C for 1 to 2 days, then subcultured, and when the color of the medium turned ...

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Abstract

The invention discloses a method for comparative analysis of virulence of different sheep mycoplasma ovipneumoniae strains. The method includes: respectively inoculating resurgent sheep mycoplasma tobe analyzed comparatively onto different chicken embryos, putting the chicken embryos after being inoculated into an incubator for culture, regularly detecting state and death conditions of the chicken embryos, recording chicken embryo lethality rate and inoculation 7d death rate of each strain, and combining identifying results of dead chicken embryos allantoic fluid sheep mycoplasma ovipneumoniae for comparative judgment. The method has the advantages of low cost, easiness in getting experimental animal SPF chicken embryos, simplicity and convenience in operation, short experiment period andhigh repeatability and overcomes the defects of high price of experimental animals, high requirements on the experimental animals, troublesome animal feeding, long experiment period and low repeatability in the prior art.

Description

technical field [0001] The invention relates to a method for comparing certain properties of different microbial strains of the same species, in particular to a method for comparing and analyzing the virulence of different Mycoplasma ovis strains. Background technique [0002] Mycoplasma ovis pneumoniae is a pathogen that can infect sheep and goats. It mainly causes sheep respiratory diseases such as cough, wheezing, runny nose, etc. The disease is prevalent all over the world. Mycoplasma ovis pneumonia can exist in normal sheep nasal cavity. In recent years, the reports of Mycoplasma pneumoniae infection in sheep in my country have been increasing, seriously endangering the healthy development of the sheep industry. Vaccine immunization is an important means to prevent and control the disease, currently mainly inactivated vaccines. [0003] There are many genotypes of Mycoplasma pneumoniae in ovis, and there are great differences in virulence. There are also differences ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12R1/35
CPCG01N33/5014C12N2503/02G01N2500/10G01N2333/30
Inventor 郝华芳储岳峰陈胜利颜新敏袁婷刘永生
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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