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Detection method for Rumex madaio Makino traditional Chinese medicine decoction piece

A technology of traditional Chinese medicine decoction pieces and detection methods, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of uncontrollable internal quality and simple detection items, and achieve the effect of perfecting detection standards, ensuring drug quality, and strong operability.

Inactive Publication Date: 2020-02-25
北京紫云腾中药饮片有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] Rheum rhubarb is a commonly used Chinese herbal medicine in Beijing, but the current edition of the Pharmacopoeia of the People’s Republic of China has not included it. Although the 1998 edition of the “Beijing Standards of Chinese Medicinal Materials” and the 2008 edition of the “Beijing Standards for the Processing of Chinese Herbal Pieces” have included rhubarb rhubarb, but The testing items are simple, only qualitative identification, but no content determination items, resulting in uncontrollable internal quality

Method used

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  • Detection method for Rumex madaio Makino traditional Chinese medicine decoction piece
  • Detection method for Rumex madaio Makino traditional Chinese medicine decoction piece
  • Detection method for Rumex madaio Makino traditional Chinese medicine decoction piece

Examples

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Effect test

Embodiment 1

[0069] The detection method of rhubarb traditional Chinese medicine decoction pieces includes the following steps:

[0070] S1 preparation of reference substance solution:

[0071] Precisely weigh 5.33mg of aloe-emodin, 5.80mg of rhein, 4.98mg of emodin, 5.25mg of chrysophanol, and 5.33mg of emodin methyl ether with a one-hundred-thousandth balance, put them in 25ml measuring bottles respectively, add methanol to dilute to the mark , accurately measure 5ml of emodin methyl ether solution, accurately measure 10ml of each of the other reference substance solutions, put them in 25ml measuring bottles, add methanol to dilute to the mark, and accurately measure 4ml of each of the above five kinds of reference substance solutions, and put them in 20ml volume Mix well in the bottle.

[0072] The concentration of the obtained reference substance solution is: aloe-emodin 16.766ug / ml, rhein 18.560ug / ml, emodin 15.729ug / ml, chrysophanol 16.800ug / ml, emodin methyl ether 8.409ug / ml refere...

Embodiment 2

[0081] This embodiment is carried out according to the method in embodiment 1, the difference is that in step S2:

[0082] Accurately weigh 0.1513g of the test product powder, put it in a stoppered Erlenmeyer flask, accurately add 25ml of methanol, weigh it, heat to reflux for 60min, weigh again, make up the lost weight with methanol, and filter. Accurately measure 5ml of the subsequent filtrate, put it in a flask, evaporate the solvent, add 10ml of hydrochloric acid solution with a mass concentration of 8%, ultrasonicate for 2min, and then add 10ml of chloroform.

[0083] In the present embodiment, the chromatogram of total anthraquinone need testing solution is shown in Figure 4 ,same Figure 1-3 , Figure 4 The five detection peaks correspond to aloe-emodin, rhein, emodin, chrysophanol and emodin methyl ether respectively from left to right. In this example, the calculation results of the individual contents of the five components and the total anthraquinone contents in...

Embodiment 3

[0085] This embodiment is carried out according to the method in embodiment 1, the difference is that in step S2:

[0086] Accurately weigh 0.1598g of the test product powder, put it in a stoppered Erlenmeyer flask, accurately add 30ml of methanol, weigh it, heat to reflux for 40min, weigh again, make up the lost weight with methanol, and filter. Precisely measure 5ml of the subsequent filtrate, put it in a flask, evaporate the solvent, add 8ml of hydrochloric acid solution with a mass concentration of 8%, ultrasonicate for 2min, and then add 8ml of chloroform.

[0087] In the present embodiment, the chromatogram of total anthraquinone need testing solution is shown in Figure 5 ,same Figure 1-4 , Figure 5 The five detection peaks correspond to aloe-emodin, rhein, emodin, chrysophanol and emodin methyl ether respectively from left to right. In this example, the calculation results of the individual contents of the five components and the total anthraquinone contents in th...

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Abstract

The invention discloses a detection method for a Rumex madaio Makino traditional Chinese medicine decoction piece, and belongs to the technical field of traditional Chinese medicine analysis. The detection method includes the following steps: preparing a mixed solution of aloe-emodin, rhein, emodin, chrysophanol and physcion control solutions; adding methanol to Rumex madaio Makino traditional Chinese medicine decoction piece powder, heating to reflow, volatilizing a solvent in a filtrate, adding a hydrochloric acid solution to hydrolyze, then adding trichloromethane to extract, taking a trichloromethane solution, recycling the solvent by decompression to be dry, and dissolving a residue into the methanol to obtain a total anthraquinone test solution; adding the methanol to the Rumex madaio Makino traditional Chinese medicine decoction piece powder, heating to reflow and filtering to obtain a free anthraquinone test solution; respectively injecting the above three solutions into a highperformance liquid chromatograph for determination; and calculating contents of total anthraquinone and free anthraquinone in the Rumex madaio Makino traditional Chinese medicine decoction piece according to a chromatogram. The detection method provided by the invention overcomes a defect that an original standard can only make a qualitative identification, makes up a blank in quantitative analysis of the Rumex madaio Makino traditional Chinese medicine decoction piece, and is innovative.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a detection method for rhubarb traditional Chinese medicine decoction pieces. Background technique [0002] Rumex is derived from the dry root of Rumex patientia L. or Rumex crispus L., a plant of Polygonaceae. The earliest record of rhubarb in the medical books of the past dynasties was the "Tujing Materia Medica" compiled by Su Song in the Song Dynasty. Later, the "Compendium of Materia Medica" compiled by Li Shizhen in the Ming Dynasty described the rhubarb rhubarb and the current Rumex. In the Qing Dynasty, Wu Qijun (sound Tongjun) continued to use this record when he compiled "Plant Research on Plant Names and Facts". Since the 1960s, rhubarb of the genus Rumex has become the mainstream of this variety in the Beijing medicinal material market, from the 1986 edition of "Beijing Traditional Chinese Medicine Processing Standards" to the 1998 editi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06
CPCG01N30/06G01N2030/027
Inventor 孟燕刘玉玲杨蕊王欣欣刘静陈光宋晓斐石渊博林青张雪芹李国兴
Owner 北京紫云腾中药饮片有限公司
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