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Cu-amino acid composite up-conversion nano material and preparation method thereof

A composite nanomaterial and nanomaterial technology, applied in the field of upconversion fluorescence imaging Cu-amino acid composite upconversion nanomaterial and its preparation, can solve the problems of CDT's wide application limitation and other issues

Active Publication Date: 2020-02-21
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These have caused great limitations to the wide application of CDT

Method used

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  • Cu-amino acid composite up-conversion nano material and preparation method thereof
  • Cu-amino acid composite up-conversion nano material and preparation method thereof
  • Cu-amino acid composite up-conversion nano material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Preparation of up-conversion nanoparticles and Cu-amino acid complex composite nanomaterials.

[0049] Step 1. Preparation of up-conversion nanoparticles β-NaYF 4 :Yb 3+ ,Er 3+ Up-conversion nanoparticles:

[0050] Put 1.0mmol ErCl in a 100mL three-necked flask 3 (RE=0.78Y+0.2Yb+0.02Er) mixed with 15mL ODE and 10mL OA. Heat to 140℃ to obtain a homogeneous solution. After cooling to room temperature, add 10mL containing 0.15g NH 4 After the methanol solution of F and 0.1g NaOH, the temperature was raised to 70°C and stirred at this temperature for 10 min. After that, the stirred solution was heated to 110°C and degassed for 30 min. Then in N 2 Heat to 300℃ under protection, react for 1h. After the reaction system was cooled to room temperature, absolute ethanol was added to precipitate the nanoparticles, centrifuged, washed with absolute ethanol several times, and finally dissolved in 10 mL cyclohexane for later use. The TEM picture of the upconversion nanoparti...

Embodiment 2

[0060] Example 2: Preparation of Cu-amino acid composite upconversion nanomaterial with glucose oxidase immobilized on the surface.

[0061] Refer to Case 1 for steps 1 to 5.

[0062] Step 6: The amino group on the surface of the composite nanomaterial and the carboxyl group on the glucose oxidase form an amide bond to connect the two together to finally obtain the material UCNPs@Cu-Cys-GOX.

[0063] Add 0.1mg EDC, 0.3mg NHS and 0.2mg GOX to 3mL PBS, stir for 40min, then add 20mg UCNPs@Cu-Cys, continue stirring for 1h, centrifuge to collect the solid product (8000rpm, 3min), and wash twice with PBS. The composite nanomaterial UCNPs@Cu-Cys-GOX loaded with glucose oxidase was obtained, and finally dispersed in PBS (pH=6.0, 50mM) and stored at 4°C.

[0064] After getting the material UCNPs@Cu-Cys-GOX, first react it with glutathione to test the change in the valence state of copper ions in Cu-Cys and the ability to recover upconversion fluorescence. UCNPs@Cu-Cys-GOX was mixed with diffe...

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Abstract

The invention belongs to the field of nano-biomedicine, and relates to a Cu-amino acid composite up-conversion nano material capable of realizing chemical kinetic therapy and near-infrared light-activated up-conversion fluorescence imaging simultaneously and a preparation method of the Cu-amino acid composite up-conversion nano material. The Cu-amino acid composite up-conversion nano material cansimultaneously realize the chemical kinetic therapy and the near-infrared light-activated up-conversion fluorescence imaging, and has a uniform sphere shape and a clear core-shell structure; the innercore is rare earth fluoride nanoparticles, and the outer shell is a Cu-L-cysteine complex; the Cu-amino acid composite up-conversion nano material can be enriched in tumor sites through an EPR effect, and is specifically activated by the tumor microenvironment without causing damage to normal tissues; the Cu-amino acid composite up-conversion nano material can consume reduced glutathione over-expressed in cells and release cuprous ions with Fenton-like reactivity, and at the same time, an efficient Fenton reaction is performed to produce .OH having strong cytotoxicity; and under the irradiation of near-infrared light, the Cu-amino acid composite up-conversion nano material can emit ultraviolet and visible light, and realize the imaging function that an ordinary chemical kinetic reagent does not have.

Description

Technical field [0001] The invention belongs to the field of nano-biomedicine, and relates to an up-conversion fluorescent imaging Cu-amino acid composite up-conversion nano material capable of simultaneously realizing chemical kinetic treatment and near-infrared light activation and a preparation method thereof. Background technique [0002] Malignant tumors are one of the important diseases threatening human health. The traditional treatment methods mainly include surgery, radiotherapy and chemotherapy. Chemotherapy is a systemic treatment with the purpose of killing tumor cells, but because it is not targeted, it can cause damage to normal tissues and organs and cause serious side effects. At the same time, there are problems such as fast metabolism and multi-drug resistance. The high permeability and high retention (EPR effect) of the tumor site, as well as the acidity, higher concentration of glutathione (GSH) and hydrogen peroxide (H 2 O 2 ) The microenvironment has arouse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/85A61K33/34A61K38/44A61K49/00A61P35/00B82Y40/00B82Y30/00
CPCC09K11/02C09K11/7773A61K33/34A61K38/443C12Y101/03004A61K49/0019A61P35/00B82Y40/00B82Y30/00A61K2300/00
Inventor 李春霞陈晴吕光磊王曼沈扬王冬梅
Owner ZHEJIANG NORMAL UNIVERSITY
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