Microfluidic chip and microfluidic cell sorting system

Abstract

The invention relates to a microfluidic chip and a microfluidic cell sorting system. A cell sample sequentially passes through a liquid flow gathering area, an optical detection area and a jet flow sorting area on the microfluidic chip, and then is sorted to obtain target cells; and the jet flow sorting area comprises at least one jet flow pool, at least one surface of the jet flow pool is of a membrane structure, the volume of the jet flow pool is changed by the change of the membrane structure, jet flow is generated, and the jet flow impacts the cells to enter different outlets. Meanwhile, the microfluidic cell sorting system provided by the invention comprises the microfluidic chip, a sample injection system, an optical detection system, a jet flow sorting system and a control system. The technology provided by the invention has small influence on cell activity, the microfluidic chip can be replaced, and cross contamination is not generated; and the microfluidic chip adopts a two-layer design, the processing is simple and cheap, the jet flow pool and the membrane structure are directly made of materials such as glass forming the microfluidic chip, and when the processing of themicrofluidic chip is completed, the jet flow sorting structure is completed, the membrane structure does not need to be additionally processed, and the process is simpler.

Description

technical field [0001] The invention relates to the technical field of cell sorting systems related to flow cytometry, in particular to a microfluidic chip and a microfluidic cell sorting system. Background technique [0002] Flow cytometry is a technique for multi-parameter analysis and sorting of the characteristics and components of single cells in fast linear flow or other various tiny particles and their loads using flow cytometry. It can not only measure cell size , the shape of internal particles, can detect cell surface and cytoplasmic antigens, intracellular DNA and RNA content, etc., and analyze population cells at the single-cell level. It is characterized by fast detection speed, many measurement parameters, and a large amount of collected information. Comprehensive analysis and flexible methods are one of the powerful research methods in the field of biomedicine. Existing microfluidic cell sorting schemes usually use microfluidic chips to separate cells by elec...

AI Technical Summary

Problems solved by technology

Using piezoelectric crystal high-frequency vibration to separate cells on the microfluidic chip will cause certain damage to the cells; the cell sorting process is carried out in the air, and it is easy to generate aerosol pollution containing cells, bacteria, viruses and other samples; On the one hand, in the design of microfluidic chips, additional components such as metal sheets need to be added. The process is relatively complicated, and it is easy to introduce pollution sources in the environment, which is not conducive to mass production, and the action lasts for a long time, which limits the sorting speed.

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