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Chocolate agar plate and preparation method thereof

An agar plate, chocolate technology, applied in the direction of bacteria, etc., can solve the problems of not very comprehensive and uniform nutrients, agglomeration, excessive heating, etc.

Inactive Publication Date: 2020-02-14
中秀科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing chocolate-colored agar medium is not very comprehensive and uniform in nutrient composition, and has poor repair ability for damaged bacteria, and it is difficult to meet more and more stringent tests
Moreover, the existing chocolate agar preparation method is more suitable for small-scale self-production and self-use, and there will be agglomeration, overheating, etc. during large-scale production, which will affect product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] The embodiment of the present invention also provides a kind of preparation method of above-mentioned chocolate agar plate, it comprises:

[0031] S1. Mix tryptone, sodium chloride, yeast extract powder, beef heart extract powder, agar, and water, heat and dissolve to obtain a culture solution;

[0032] S2. Sterilize the culture medium at 120-125°C for 20-40 min;

[0033] S3. Cool down the sterilized culture solution to 45-50°C and mix it with the defibrated sheep blood to obtain a mixed solution;

[0034] S4. Heat the mixed solution at 80-85°C for 5-10 min, then cool down to 60-70°C and continue heating for 0.5-1 h to obtain a chocolate-colored solution;

[0035] S5. Pour the chocolate-colored solution into a petri dish to cool down to obtain a chocolate-colored agar plate.

[0036] Furthermore, tryptone, yeast extract powder, and beef heart extract powder provide suitable carbon and nitrogen sources for microorganisms. Sodium chloride can maintain the balance of ba...

Embodiment 1

[0044]This embodiment provides a chocolate-colored agar plate, which is obtained by mixing culture fluid and defibrated sheep blood, heating, cooling and solidifying. Wherein, in parts by weight, the culture solution includes:

[0045] 14 parts of tryptone, 6 parts of sodium chloride, 0.5 parts of yeast extract powder, 8 parts of beef heart extract powder, 13 parts of agar, and 1000 parts of water;

[0046] The volume ratio of culture medium and defibrated sheep blood is 1:0.08.

[0047] The preparation method of this chocolate color agar plate is as follows:

[0048] S1. Mix tryptone, sodium chloride, yeast extract powder, beef heart extract powder, agar, and water, heat to 90°C to dissolve to obtain a culture medium, and adjust the pH of the culture medium to 7.2.

[0049] S2. Sterilize the culture medium at 120°C for 40 min.

[0050] S3. Cool down the sterilized culture medium to 45° C., and mix it evenly with the defibrated sheep blood preheated to 40° C. to obtain a mi...

Embodiment 2

[0056] This embodiment provides a chocolate-colored agar plate, which is obtained by mixing culture fluid and defibrated sheep blood, heating, cooling and solidifying. Wherein, in parts by weight, the culture solution includes:

[0057] 18 parts of tryptone, 4 parts of sodium chloride, 2 parts of yeast extract powder, 4 parts of beef heart extract powder, 18 parts of agar, and 900 parts of water;

[0058] The volume ratio of culture medium and defibrated sheep blood is 1:0.1.

[0059] The preparation method of this chocolate color agar plate is as follows:

[0060] S1. Mix tryptone, sodium chloride, yeast extract powder, beef heart extract powder, agar, and water, heat to 80°C to dissolve to obtain a culture medium, and adjust the pH of the culture medium to 7.6.

[0061] S2. Sterilize the culture medium at 125°C for 20 min.

[0062] S3. Cool down the sterilized culture medium to 50°C, and mix it evenly with the defibrated sheep blood preheated to 45°C to obtain a mixed sol...

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PUM

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Abstract

The invention relates to the technical field of culture media, in particular to a chocolate agar plate, which is more comprehensive in nutrition and capable of effectively promoting growth of corresponding bacteria and increasing the separation rate of the corresponding bacteria. According to a preparation method of the chocolate agar plate, after a culture solution is subjected to high-temperature sterilization, a mixed solution is subjected to heating hemolysis, so that a factor V and a factor X in red blood cells are released. During heating, the substance is heated for a short time at a relatively high temperature to promote rapid rupture of cell walls, and then is continuously heated at a slightly low temperature, so that other nutrient substances are prevented from being destroyed byhigh temperature while substances in red blood cells are fully released. The preparation method is simple to operate, has low requirements on equipment, can effectively improve the product quality, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of medium, in particular to a chocolate agar plate and a preparation method thereof. Background technique [0002] Pathogenic bacteria or opportunistic pathogenic bacteria exist in the infected site, leading to common clinical infections, such as respiratory tract infection, reproductive tract infection, intestinal infection, etc. In order to accurately diagnose the type of bacteria in the infected site, patients are often used clinically Samples are collected from the site for culture, and clinical examiners select suspicious strains for identification based on clinical symptoms, so as to determine the type of infected bacteria in the patient's site and provide a basis for clinical treatment. Diagnosis and culture of infected bacterial types in patient parts are currently the only feasible methods in clinical diagnosis. Other methods, such as immune antigen detection and nucleic acid detection, are not suit...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 孙月鹏张娟丽吕斌谭韦丽罗江卫
Owner 中秀科技股份有限公司
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