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Y-chromosome microdeletion detection primer group and kit

A technology for chromosome microdeletion and detection kit, which is applied in the direction of microbial determination/inspection, recombinant DNA technology, biochemical equipment and methods, etc. It can solve problems such as male infertility and abnormal sperm, and achieve accurate results, flexible design, Detect fast effects

Pending Publication Date: 2020-02-11
苏州恩科金生物科技有限公司
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Problems solved by technology

Subsequently, a large number of studies have confirmed that there are genes controlling spermatogenesis on the Y chromosome Yq11.23, and AZF is divided into three regions, namely AZFa, AZFb and AZFc, and each region contains several AZF candidate genes, such as USP9Y and DBY in AZFa region, RBMY1 and HSFY in AZFb region, DAZ in AZFc, etc., the deletion or abnormality of these genes may lead to abnormal sperm production, resulting in male infertility

Method used

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  • Y-chromosome microdeletion detection primer group and kit
  • Y-chromosome microdeletion detection primer group and kit

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Effect test

Embodiment 10

[0064] Example 10 Gene detection of Y chromosome microdeletion in infertile male patients

[0065] This application collected blood samples from 10 male patients who complained of infertility from Suzhou Huizhen Medical Laboratory (all signed informed consent).

[0066] 1. DNA extraction

[0067]Use Wizard Genomic DNA purification Kit (Promega) or NucleoSpin Tissue (MN) or similar products to extract DNA from 10 blood samples (sample number 1-10). Quantification by spectrophotometer, quality inspection by agarose gel electrophoresis, and genomic DNA electrophoresis bands are usually not less than 20kb.

[0068] 2. Preparation of PCR reagents

[0069] The 19 STS loci were amplified in 5 groups (A, B, C, D, E). The grouping information is shown in Table 2, and the internal control primer ZFX / Y was added to each group.

[0070] Table 2. PCR amplification groups

[0071]

[0072] The PCR amplification reaction system is as follows:

[0073]

[0074] Add the samples into...

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Abstract

The invention discloses a Y-chromosome microdeletion detection primer group and kit. As for 19 Y-chromosome microdeletion mutation sites, an X / Y linked zinc finger protein gene (ZFX / Y) is selected asan internal control site. The Y-chromosome microdeletion detection primer group and kit can overcome defects of the prior art in the aspects of operability, sensitivity and time saving, are flexible in technical experimental design, cover more mutation sites, and are more suitable for Chinese people, accurate in results, high in detection speed and suitable for batch screening.

Description

technical field [0001] The invention belongs to the technical field of biological gene detection, and relates to a primer set and a kit for detecting Y chromosome microdeletion. Background technique [0002] About 15% of couples in the world are infertile, and the factor of male infertility accounts for about 50%. Y chromosome microdeletion is the second most common cause of male idiopathic infertility after Klinefelter syndrome. In 1976, scientists discovered azoospermia factor (AZF), which they believed to be a gene locus on the long arm of the Y chromosome that controls sperm production. Subsequently, a large number of studies have confirmed that there are genes controlling spermatogenesis on the Y chromosome Yq11.23, and AZF is divided into three regions, namely AZFa, AZFb and AZFc, and each region contains several AZF candidate genes, such as USP9Y and DBY in the AZFa region, RBMY1 and HSFY in the AZFb region, DAZ in the AZFc, etc., the deletion or abnormality of thes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 张惠丹
Owner 苏州恩科金生物科技有限公司
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