Kit and method for quantitatively detecting five fat-soluble vitamins in blood plasma

Abstract

The invention discloses a kit and method for quantitatively detecting five fat-soluble vitamins in blood plasma. The kit comprises a fat-soluble vitamin calibrator, an isotope mixed standard substance, a fat-soluble vitamin quality control substance, an instrument quality control substance and a mobile phase additive. According to the invention, a solid-phase support liquid-liquid extraction method is adopted and is matched with an automatic pipetting station, so a sample pretreatment method is greatly simplified, and the aim of simultaneously and accurately detecting the five fat-soluble vitamins at high flux and high speed is fulfilled by combining a liquid chromatography-tandem mass spectrometry method. According to the invention, during the detection of a peripheral blood plasma sample, five fat-soluble vitamins can be quantified at a time only by about 250 microliters of plasma sample; moreover, due to a fact that the pretreatment method is simple and quick, the operation time issaved, the content of the fat-soluble vitamins in a human body can be effectively diagnosed and detected according to the indexes, and diseases caused by lack or excess of the fat-soluble vitamins canbe prevented and diagnosed in time. Therefore, the kit and method have extremely high application values in clinical, health examination and scientific research.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit and method for quantitatively detecting five fat-soluble vitamins in blood plasma. Background technique [0002] Fat-soluble vitamins are naturally occurring micronutrients in food, which provide and maintain essential and extensive physiological and biochemical functions for the human body. To meet human health and metabolic needs, fat-soluble vitamins must be obtained from the daily diet. Fat-soluble vitamins are vitamins that are soluble in organic solvents but not in water, including vitamin A, vitamin D, vitamin E, and vitamin K 1 . [0003] Vitamin D, also known as calciferol, anti-rickets vitamin, is a steroid derivative, mainly in the human body as 25 hydroxyvitamin D 2 and 25 hydroxyvitamin D 3 Vitamin A, also known as retinol, can promote cell proliferation and growth, and protect the integrity and integrity of the epithelial tissue structure of various organs. H...

AI Technical Summary

Problems solved by technology

In the process of determining analytes by immunoassay, the antibodies used can also recognize derivatives of other vitamins, and usually the antigen-antibody response cannot reach 100%.

Therefore, the results of this method have large differences, poor specificity, and poor reliability when applied to clinical testing, especially for vitamins with low detection content.

Due to the limitation of its detection limit, liquid chromatography is only aimed at the determination of vitamins with higher content or the determination of a single vitamin

[0006] The existing domestic kits are mainly aimed at a single fat-soluble vitamin, and there is no kit for the common detection of multiple fat-soluble vitamins, and the detection method is poor in selectivity

For the existing detection methods of fat-soluble vitamins, the protein precipitation method and liquid phase extraction method are mainly used for sample pretreatment, which takes a long time, cumbersome operation, high technical requirements for personnel, and cannot be used with automation

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