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Halomona with aerobic denitrification function, and application of halomona

A technology of aerobic denitrification and halomonas, which is applied in the direction of bacteria, chemical instruments and methods, and methods based on microorganisms, can solve problems such as limited effects, achieve strong salt tolerance, solve nitrogen pollution, and denitrify good effect

Inactive Publication Date: 2020-02-07
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such strains have limited effect in high-salinity environments

Method used

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  • Halomona with aerobic denitrification function, and application of halomona
  • Halomona with aerobic denitrification function, and application of halomona
  • Halomona with aerobic denitrification function, and application of halomona

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 is used to illustrate the screening and identification process of strains.

[0033] 1. Screening of Halomonas with high salt tolerance and aerobic denitrification function

[0034] 1.1 Source of strain

[0035] The test strains were isolated from the RAS biological aerated filter of the Key Laboratory of Fisheries of the Ministry of Education of Dalian Ocean University.

[0036] 1.2 Strain screening

[0037] After the biofilter from the biofilter was washed with sterile water, the biofilm was scraped off with a sterilized blade, and diluted with sterilized normal saline for 10 -1 -10 -7 , and then uniformly spread on LB solid medium, and cultivated at 30°C for 24h. Pick a single clone colony and inoculate it in denitrification medium (Denitrification Medium, DM) seawater culture medium for 48h, then streak the bacterial liquid on bromothymol blue (Bromothymol Blue, BTB) solid medium, culture at 30°C for 3d . Select the monoclonal strains that turn blue o...

Embodiment 2

[0055] Example 2 is used to illustrate the effect of dissolved oxygen (DO) on the aerobic denitrification performance of Halomonas CGMCC No.18499.

[0056] Draw the bacterial suspension in the logarithmic growth phase (OD 600 About 1.0) 1mL was inoculated into a 100mL vial containing 50mL sterilized seawater (salinity: 31) DM medium, measured by KNO 3 As the only nitrogen source, set the initial nitrate nitrogen concentration to 100mg L -1 , and then respectively at 90rpm (dissolved oxygen concentration is 5.6 ± 0.10mg / L), 120rpm (dissolved oxygen concentration is 6.9 ± 0.18mg / L) and 150rpm (dissolved oxygen concentration is 7.5 ± 0.39mg / L) conditions. The rest of the experimental conditions were set as follows: the carbon source was sodium acetate, the C / N was 10, 30°C, and the pH value was 7.0±0.5, cultured for 48 hours, and each treatment was set in 3 parallels, and samples were taken every 4 hours. The culture medium was taken for optical density (Optical Density, OD 60...

Embodiment 3

[0063] Example 3 is used to illustrate the effect of C / N on the aerobic denitrification performance of Halomonas CGMCC No.18499.

[0064] Draw 1mL of the bacterial suspension in the logarithmic growth phase (OD600 is about 1.0) according to the inoculum size of 2% and inoculate it into a 100mL vial containing 50mL of sterilized seawater (salinity is 31) DM medium, and use KNO 3 As the only nitrogen source, set the initial nitrate nitrogen concentration to 100mg L -1 , the carbon source is sodium acetate, and the control C / N is 3, 5, 10, 15 and 20, respectively. The rest of the experimental conditions were all set at 30°C, the pH value was 7.0±0.5, and the rotation speed was 150rpm (dissolved oxygen concentration was 7.5±0.39mg / L) and cultured for 48h. Three parallels were set up for each treatment, samples were taken every 4h, and the optical density (Optical Density, OD) of the culture solution was taken. 600 ), TN, NO 3 - -N, NO 2 - -N, NH 4 + -N and TOC index determ...

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Abstract

The invention discloses halomona with an aerobic denitrification function, and application of the halomona. The halomona is collected in the China General Microbiological Culture Collection Center on17th September, 2019 with a collection number of CGMCC No.18499. The halomona can carry out aerobic denitrification nitrogen removal under a high-salinity environment and can comprise the following steps of: 1) preparing a high-salinity nitrogen-containing liquid culture medium, wherein salinity is 31; and 2) taking a halomona strain, inoculating the halomona strain in the culture medium accordingto an inoculum size a, and culturing for 24-48h under conditions of 90-150 rpm, 20-40 DEG C and pH of 7.0+ / -0.5, wherein a is greater than or equal to 1% and less than or equal to 3%. When the halomona is used for processing the nitrogen-containing waste water of seawater, a removal rate for NO3<->-N at 48h is 92%, a removal rate for TOC (Total Organic Carbon) and a removal rate for TN (Total Nitrogen) are independently 69.4% and 65.6%, and the halomona performs efficient nitrogen removal performance in high-salinity nitrogen-containing wastewater processing.

Description

technical field [0001] The invention relates to the technical field of microbial strains, in particular to a halomonas with aerobic denitrification function and application thereof. Background technique [0002] In recent years, my country's aquaculture industry has developed rapidly, and aquaculture has provided human beings with abundant aquatic resources, but the serious environmental problems brought about by the rapid development of the industry cannot be ignored. Recirculating Aquaculture System (RAS) is generally considered to be an effective way to solve environmental pollution and increase the yield of aquatic products. The treatment and reuse of aquaculture wastewater is the key process of RAS, and one of the technical problems is how to reduce the concentration of nitrogen in the aquaculture water. Biological Aerated Filter (BAF) is the core unit commonly used in RAS water treatment, and it is considered to be able to effectively reduce excessive ammonia nitrogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38C02F103/08
CPCC02F3/34C02F2101/38C02F2103/08C12N1/205C12R2001/01
Inventor 韩蕊刘鹰宋婧吴英海任效忠任纪龙马洪婧
Owner DALIAN OCEAN UNIV
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