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Separating preparation method of phenolic acid compounds in Echinacea purpurea

An echinacea and compound technology, which is applied in the separation/purification of carboxylic acid compounds, the separation/purification of carboxylic acid esters, organic chemistry, etc., can solve the problems of separation efficiency, large injection volume, separation efficiency and product purity to be improved, etc. Achieve universality, easy large-scale production, and high purity

Active Publication Date: 2020-02-04
SHANDONG ANALYSIS & TEST CENT
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Problems solved by technology

This technology has high separation efficiency, good reproducibility, large sample volume, and no irreversible adsorption of solid stationary phase. It has been widely used in the separation and preparation of dissociable compounds such as organic acids and alkaloids. However, the existing pH The separation efficiency and product purity of various phenolic acids in Echinacea purpurea still need to be improved by zonal countercurrent chromatography

Method used

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  • Separating preparation method of phenolic acid compounds in Echinacea purpurea
  • Separating preparation method of phenolic acid compounds in Echinacea purpurea
  • Separating preparation method of phenolic acid compounds in Echinacea purpurea

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Embodiment 1

[0035] 1 Experimental part

[0036] 1.1 Instruments and reagents

[0037] The instruments used in the pH zone countercurrent chromatographic separation mainly include TBE-300C high-speed countercurrent chromatograph, TBP-5002 infusion pump, DC-0506 low temperature constant temperature bath (the above instruments were purchased from Shanghai Tongtian Biotechnology Co., Ltd.) , 8823B ultraviolet detector (Beijing Binda Yingchuang Technology Co., Ltd.), 3057-11 portable recorder (Chongqing Chuanyi Automation Co., Ltd.) and UB-7pH meter (Denver Instrument Co., Ltd.); the analysis and identification of samples are mainly based on Waterse2695 high-performance liquid chromatograph equipped with 2998PDA detector, quaternary pump, column thermostat and autosampler (Waters Technology Co., Ltd., USA), Bruker ADVANCE DPX 400 nuclear magnetic resonance spectrometer (Bruker, Switzerland).

[0038] The reagents used for the extraction and separation of Echinacea purpurea were all analytical...

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Abstract

The invention relates to a separating preparation method of phenolic acid compounds in Echinacea purpurea. The preparation method comprises the steps: performing segment enrichment of the phenolic acid compounds in Echinacea purpurea firstly by using the property that the elution rate can be accelerated by an eluent in pH zone countercurrent chromatography, and then using the pH zone countercurrent chromatography separation on Echinacea purpurea so as to obtain eight high-purity phenolic acid compounds including caftaric acid, fertaric acid, coutaric acid, iso-fertaric acid, methyl caffeoyltartrate, chicoric acid (2,3-O-dicaffeoyltartaric acid), ferulic acid and methyl caffeate. Through the method, not only can a high injection volume be ensured, but also lower-content compounds are enriched after segment enrichment is performed, so that more compounds are obtained during second separation, and the separation efficiency is improved greatly.

Description

technical field [0001] The invention belongs to the field of purification and separation of phenolic acid compounds in Echinacea purpurea, in particular to a method for separating and preparing phenolic acid compounds in Echinacea purpurea. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Echinacea purpurea (Echinacea purpurea) is a perennial herbaceous plant of the genus Echinacea in the Asteraceae family. It is native to North America and has been introduced in China. Echinacea has a long history of medicinal use in the West. It has been developed into tinctures, tablets, capsules and other dosage forms, and is mostly used for the treatment of infectious diseases, such a...

Claims

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Application Information

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IPC IPC(8): C07C67/48C07C69/732C07C69/734C07C59/64C07C51/42A61P15/00A61P31/00A61P17/02A61P31/04A61P7/00A61P11/00
CPCC07C67/48C07C51/42A61P15/00A61P31/00A61P17/02A61P31/04A61P7/00A61P11/00C07C69/732C07C69/734C07C59/64
Inventor 王晓马天宇王岱杰耿岩玲刘伟
Owner SHANDONG ANALYSIS & TEST CENT
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