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Llinked autosomal (STR typing system based on high-throughput sequencing technology and kit

A technology of autosomes and kits, applied in recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems such as inability to distinguish complex kinship

Active Publication Date: 2020-01-31
SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most linked STR studies are X-STR and Y-STR genetic markers, which require special kinship to be applied; independently inherited autosomal STRs cannot distinguish complex kinship

Method used

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  • Llinked autosomal (STR typing system based on high-throughput sequencing technology and kit
  • Llinked autosomal (STR typing system based on high-throughput sequencing technology and kit
  • Llinked autosomal (STR typing system based on high-throughput sequencing technology and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1: A STR typing system for complex kinship identification and its kit

[0081] 1.1 Composition and characteristics of autosomal-linked STR loci:

[0082] (1) The STR typing system in this example includes the following 60 STR loci:

[0083] D1S2131、D1S3721、D1S2130、D1S1600、D1S1653、D1S1660、D1S3732、D2S1364、D2S2734、D2S1396、D2S428、D2S435、D2S1387、D2S1792、D2S1399、D2S2959、D3S2431、D3S4547、D4S1643、D4S2408、D4S3326、D4S2368、D5S2845、D5S1473、D5S813、 D5S1716、D5S1459、D5S1487、D5S1466、D5S2496、D5S2501、D6S1019、D6S2417、D6S2412、D6S1284、D7S1820、D7S3050、D7S821、D8S594、D8S1468、D8S2322、D8S569、D8S1475、D9S746、D9S319、D9S2149、D11S2010、D11S1392、D12S376、D12S1052、 D13S317, D13S790, D14S1432, D14S1428, D15S644, D15S1507, D16S752, D16S485, D20S481 and D20S1151;

[0084] It has the following measurable properties:

[0085] 1) The repeating unit of the core region is a tetranucleotide;

[0086] 2) The heterozygosity of each STR is greater than 0.6;

[0087] 3) The number of STRs contained in eac...

Embodiment 2

[0107] Example 2: A STR typing system for complex kinship identification and its kit

[0108] 2.1 Composition and characteristics of autosomal-linked STR loci:

[0109] (1) The STR typing system in this example includes the following 58 STR loci:

[0110]D1S3736、D1S1665、D1S2127、D1S2138、D1S1642、D1S518、D1S1604、D1S3729、D1S3727、D2S1336、D2S1779、D2S1771、D2S437、D2S2970、D2S2944、D2S1327、D3S2402、D3S1766、D4S2411、D4S3351、D4S243、D4S2426、D4S2373、D5S1490、D5S2856、 D5S2495、D5S2855、D5S1722、D5S1725、D6S1048、D6S1275、D7S796、D7S1799、D8S2324、D8S2330、D8S1144、D9S745、D9S301、D9S1124、D10S1246、D10S2485、D11S1983、D11S2363、D11S1368、D12S393、D12S1063、D13S1807、D13S1492、D14S738、D14S301、 D14S583, D15S816, D15S1514, D18S872, D18S972, D18S548, D20S1145, D20S477;

[0111] It has the following measurable properties:

[0112] 1) The repeating unit of the core region is a tetranucleotide;

[0113] 2) The heterozygosity of each STR is greater than 0.6;

[0114] 3) The number of STRs contained in each group is at lea...

Embodiment 3

[0127] Example 3: A STR typing system for complex kinship identification and its kit

[0128] 3.1 Composition and characteristics of autosomal-linked STR loci:

[0129] (1) The STR typing system in this example includes the following 61 STR loci:

[0130] D1S532、D1S1611、D1S3733、D1S533、D1S1614、D2S2977、D2S1374、D2S1394、D2S2966、D2S2969、D2S1371、D2S434、D2S1338、D3S4016、D3S2388、D4S1626、D4S1653、D5S2858、D5S2796、D5S1463、D5S815、D5S2499、D5S2498、D6S1043、D6S1274、 D6S1056、D6S1013、D6S1054、D7S820、D7S2205、D7S3071、D7S2845、D8S2326、D8S1464、D8S2320、D8S1470、D8S588、D8S1471、D9S2026、D9S747、D9S2128、D10S2469、D10S1238、D11S4464、D11S4958、D13S1818、D13S767、D14S615、D14S608、D14S597、 D14S302, D14S749, D16S767, D16S3393, D18S537, D18S875, D18S1367, D20S1152, D20S206, D20S607, D20S1146;

[0131] It has the following measurable properties:

[0132] 1) The repeating unit of the core region is a tetranucleotide;

[0133] 2) The heterozygosity of each STR is greater than 0.6;

[0134] 3) The number of STRs contain...

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Abstract

The invention claims a linked autosomal short tandem repeat (STR) typing system based on a high-throughput sequencing technology and a kit, and relates to the technical field of nucleic acid in-vitrodetection. The STR typing system includes PCR primers for amplification of 61, 121 or 179 linked autosomal STR loci, and the kit includes a PCR primer combination, an Index linker sequence, an IGT-EM707 polymerase mixture, an amplification buffer enhancing agent NB, a YF buffer liquid B and a library building reagent. The STR typing system and kit provided by the invention can realize single-tubeamplification of the 61, 121 or 179 linked autosomal STR loci, have good balance, high sensitivity, good specificity, and accurate typing results, and can be used to identify complex genetic relationships of different genetic relationships at a same level.

Description

technical field [0001] The invention relates to the technical field of nucleic acid in vitro detection, in particular to a linked autosomal STR typing system based on high-throughput sequencing technology and a kit thereof. Background technique [0002] Short tandem repeat (STR) is a special sequence composed of 2-6bp repeating units in series. STR loci are large in number and widely distributed, accounting for about 3% of the entire genome, and are highly polymorphic. The main reason is that the number of core sequence repeats varies among individuals, and this difference follows the Mendelian law of inheritance in the genetic process. Therefore, STR amplification detection technology is widely used in forensic individual identification, kinship identification and population genetics research. The most commonly used STR typing techniques are fluorescent labeling multiplex amplification combined with capillary electrophoresis (CE) typing techniques and next-generation seque...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2531/113C12Q2535/122C12Q2525/191
Inventor 严江伟范庆炜张更谦张君程凤王萌春李万婷石林玉张晓梦张家榕
Owner SHANXI MEDICAL UNIV
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